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The map label for this gene is pucI [H]

Identifier: 186476291

GI number: 186476291

Start: 1730688

End: 1732199

Strand: Reverse

Name: pucI [H]

Synonym: Bphy_1533

Alternate gene names: 186476291

Gene position: 1732199-1730688 (Counterclockwise)

Preceding gene: 186476292

Following gene: 186476290

Centisome position: 49.79

GC content: 61.9

Gene sequence:

>1512_bases
ATGAAGCAGACAGCGCAATCCGCCGACGCGTCTTACGCGGCCGGCGCGCACGGCAGCAGTCTCTATAACGACGACCTTGC
GCCGACTGGCCCGGCGCAGCGCACGTGGAAGTGGTATCACTTCGCCGCGCTTTGGGTCGGCATGGTGATGAACATCGCGT
CGTACATGCTCGCGGCAGGGCTGACGGAAGAGGGCATGTCGCCGTGGCAGGCGGTGTTGACCGTGCTGCTCGGGAACATG
ATCGTGCTGGTTCCGATGCTGCTGATCGGTCATGCGGGCGCGAAGCACGGCATTCCGTACGCGGTGCTCGTGCGTTCGTC
ATTCGGCACGCAGGGCGCGAAACTGCCCGCGATGTTGCGCGCGATCGTCGCGTGCGGCTGGTACGGCATCCAGACGTGGC
TCGGTGGCAGCGCGATCTATACGCTGCTCAACATCCTGACGGGCAATGCGCTGCACGGCGCGGCGCTGCCGTTTCTCGAC
ATCTCGATTGGGCAGTTCGCGTGCTTTCTCGCGTTCTGGGCGTTGCAGATTTATTTCATCGTGCATGGCACCGATTCGAT
TCGCTGGCTCGAAAGCTGGTCCGCGCCGATCAAGGTGGTGATGTGCATCGCGCTCGTGTGGTGGGCGACGTCCAGGGCGG
GCGGCGTCGGGTCGATGCTCTCGGCGCCTTCGCAGTTCGTGCCGGGCGGCAAGAAGGAGGGCCTGTTTTGGCTCACGTTC
TGGCCCGGGCTTACGGCAATGGTTGGCTTCTGGGCGACGCTTGCGTTGAATATCCCTGACTTCACGCGTTTCGCCAGGAC
GCAGCGGGATCAGATCGTCGGGCAGTCGATCGGCTTGCCGGTGCCGATGGCTTTGCTGTCGGTGATCTCCGTGGTCGTCA
CGTCGGCGACGGTTGTGATCTACGGCAAGGCGATCTGGGACCCGATCGATCTGACGAGCCGCATGACGGGTGTCGGCGTC
GGCGTTGCACTCATCATCCTCACGCTCGATACGATGTGCTGCAATCTCGCGGCGAATCTTGTGGGTCCCGCGTATGATTT
TTCGAGCCTCTGGCCTAAGGGAATATCGTACCGCACGGGCGGGCTGATGACGGCGGTCATTGCGATCGTGATGATGCCGT
GGAAGATTCTCGCGACCACCCAAGGCTATATCTTTACGTGGCTCGTGGGTTACTCGGCGTTGCTTGGACCTGTCGCAGGC
ATCCTGATGGTCGATTACTTCCTCATTCGCGGCACGCGGCTCGACTCGCACGAACTTTTCGACGAACACGGCGAATACAG
CTATACGGGCGGCTGGAACCTGGCAGCCGTCGCCGCGCTGGTGATCGGCGTGGTTCCGAATCTGCCCGGCTTTTTGCATA
CGGCGTTTCCCGCATCGTTTCCGAACGTGCCCGCATTCTTCAATACGCTCTATACCTATGCATGGTTCGTCGGACTGGCG
TTGGCATCCATTGTGTATGGCACGTGGATGAAGCTGCGCCGCAATCCCGGCGCGAGCATGGCGAGTGCGTGA

Upstream 100 bases:

>100_bases
GCCTGAAACGGCAGACGCACGGCACCCCGCCAAGGCAGCCTGAAAAAAGCACGCTGATAAGGGCACCCCAGTCAACGAGC
GGTCAAGTGGAGAACCATCG

Downstream 100 bases:

>100_bases
TGTGATTCGATGTTTGACCGAGGAGGCAAGCGATGACAACTCTTATTCGCGGCGGCACGGTGATCGATGCCGACCGCACG
CATAGAGCCGACGTATTGAT

Product: NCS1 nucleoside transporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKQTAQSADASYAAGAHGSSLYNDDLAPTGPAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVLTVLLGNM
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISIGQFACFLAFWALQIYFIVHGTDSIRWLESWSAPIKVVMCIALVWWATSRAGGVGSMLSAPSQFVPGGKKEGLFWLTF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIVGQSIGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGVGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRTGGLMTAVIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDSHELFDEHGEYSYTGGWNLAAVAALVIGVVPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLA
LASIVYGTWMKLRRNPGASMASA

Sequences:

>Translated_503_residues
MKQTAQSADASYAAGAHGSSLYNDDLAPTGPAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVLTVLLGNM
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISIGQFACFLAFWALQIYFIVHGTDSIRWLESWSAPIKVVMCIALVWWATSRAGGVGSMLSAPSQFVPGGKKEGLFWLTF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIVGQSIGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGVGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRTGGLMTAVIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDSHELFDEHGEYSYTGGWNLAAVAALVIGVVPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLA
LASIVYGTWMKLRRNPGASMASA
>Mature_503_residues
MKQTAQSADASYAAGAHGSSLYNDDLAPTGPAQRTWKWYHFAALWVGMVMNIASYMLAAGLTEEGMSPWQAVLTVLLGNM
IVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLRAIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLD
ISIGQFACFLAFWALQIYFIVHGTDSIRWLESWSAPIKVVMCIALVWWATSRAGGVGSMLSAPSQFVPGGKKEGLFWLTF
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIVGQSIGLPVPMALLSVISVVVTSATVVIYGKAIWDPIDLTSRMTGVGV
GVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRTGGLMTAVIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAG
ILMVDYFLIRGTRLDSHELFDEHGEYSYTGGWNLAAVAALVIGVVPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLA
LASIVYGTWMKLRRNPGASMASA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=478, Percent_Identity=28.8702928870293, Blast_Score=195, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6322219, Length=449, Percent_Identity=28.2850779510022, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319429, Length=485, Percent_Identity=28.2474226804124, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319495, Length=450, Percent_Identity=27.3333333333333, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324645, Length=447, Percent_Identity=24.1610738255034, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323266, Length=444, Percent_Identity=22.7477477477477, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324766, Length=452, Percent_Identity=22.1238938053097, Blast_Score=120, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 54179; Mature: 54179

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTAQSADASYAAGAHGSSLYNDDLAPTGPAQRTWKWYHFAALWVGMVMNIASYMLAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTEEGMSPWQAVLTVLLGNMIVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
AIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLDISIGQFACFLAFWALQIYFI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHE
VHGTDSIRWLESWSAPIKVVMCIALVWWATSRAGGVGSMLSAPSQFVPGGKKEGLFWLTF
EECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCCCCCEEEEEE
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIVGQSIGLPVPMALLSVISVVVTSATVVI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
YGKAIWDPIDLTSRMTGVGVGVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRTG
EEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC
GLMTAVIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGILMVDYFLIRGTRLDSHELF
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEHGEYSYTGGWNLAAVAALVIGVVPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLA
HHCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LASIVYGTWMKLRRNPGASMASA
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKQTAQSADASYAAGAHGSSLYNDDLAPTGPAQRTWKWYHFAALWVGMVMNIASYMLAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTEEGMSPWQAVLTVLLGNMIVLVPMLLIGHAGAKHGIPYAVLVRSSFGTQGAKLPAMLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHH
AIVACGWYGIQTWLGGSAIYTLLNILTGNALHGAALPFLDISIGQFACFLAFWALQIYFI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHE
VHGTDSIRWLESWSAPIKVVMCIALVWWATSRAGGVGSMLSAPSQFVPGGKKEGLFWLTF
EECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHCCCCCCCCEEEEEE
WPGLTAMVGFWATLALNIPDFTRFARTQRDQIVGQSIGLPVPMALLSVISVVVTSATVVI
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEE
YGKAIWDPIDLTSRMTGVGVGVALIILTLDTMCCNLAANLVGPAYDFSSLWPKGISYRTG
EEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC
GLMTAVIAIVMMPWKILATTQGYIFTWLVGYSALLGPVAGILMVDYFLIRGTRLDSHELF
HHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DEHGEYSYTGGWNLAAVAALVIGVVPNLPGFLHTAFPASFPNVPAFFNTLYTYAWFVGLA
HHCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LASIVYGTWMKLRRNPGASMASA
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]