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The map label for this gene is xthA [H]

Identifier: 186476236

GI number: 186476236

Start: 1668146

End: 1668922

Strand: Direct

Name: xthA [H]

Synonym: Bphy_1478

Alternate gene names: 186476236

Gene position: 1668146-1668922 (Clockwise)

Preceding gene: 186476234

Following gene: 186476240

Centisome position: 47.95

GC content: 61.65

Gene sequence:

>777_bases
ATGAAATTAGCCACGTGGAACGTGAACTCCCTGAAGGTTCGCCAGCAGCATGTGATCGACTGGCTCGAGCTCAGCCGGAC
CGACGTGCTTTGCCTGCAGGAACTGAAACTGCCCGACGAAAAGTTCCCGCGCGCAGAGCTGGCAGAAAAGGGATATCGCA
GCTGGTTCGCGGGACAGAAGACGTACAACGGCGTCGGCATTCTCGTGCGCGACGGGGTGAGCGTCGACGAAGGCAGCATC
GTGCGCAACATCCCTGGCTTTGAAGACCCGCAGCAGCGCGTGATCGCGGCCACCGTCGAAGGCGTGCGCGTGGTCTGCGC
GTACTTTCCGAACGGCCAGGCGCCCGGCACCGACAAGTTCGCCTACAAGCTGCGCTGGCTGGACGCGATGTATGAATGGA
TCTCGAGCGAACTGACGCAGCATCCGAAGCTCGCGCTGCTCGGCGATTACAACATCGCGCCCGAAGACCGCGACGTGCAT
GATCCGAAGGCATGGGAGGGACAGAATCTCGTGTCGCCCGAAGAGCGCGCGCAGTTTAACCGTCTGATTGAACTGGGGCT
CGTCGATTCGTTCCGGCTCTTCGATCAGCCCGAGAAGACCTTTACGTGGTGGGATTACCGGATGCTCGCCTTCCGCCGCA
ACGCGGGCTTACGCATCGACCATATCCTGCTGTCGAAGCCGCTCGCTGCGAGCTGCACCCATTGCGATGTCGACAAGACG
CCGCGCAAATGGGACCAGCCTTCGGACCACGCGCCCGTCGTCGCGCTCACCGAATAA

Upstream 100 bases:

>100_bases
CCTTTTGGCTTATGCATGTTGCCGTACTGAGCCGCCCTGCCCGATCGCGGTAAGATTCCATCCTGCAACGACGCAACGAA
AACGAGCAACGAGAAGAGCG

Downstream 100 bases:

>100_bases
GCGAAGGCAGCAAAGACAAAGCGGCTCGATGGCGCATCACGCCGTCGAGCCGCTTTCGTTGTGCCACACGTGAGCGTAAG
TGCGTCACGCCCGCTCGCAT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKLATWNVNSLKVRQQHVIDWLELSRTDVLCLQELKLPDEKFPRAELAEKGYRSWFAGQKTYNGVGILVRDGVSVDEGSI
VRNIPGFEDPQQRVIAATVEGVRVVCAYFPNGQAPGTDKFAYKLRWLDAMYEWISSELTQHPKLALLGDYNIAPEDRDVH
DPKAWEGQNLVSPEERAQFNRLIELGLVDSFRLFDQPEKTFTWWDYRMLAFRRNAGLRIDHILLSKPLAASCTHCDVDKT
PRKWDQPSDHAPVVALTE

Sequences:

>Translated_258_residues
MKLATWNVNSLKVRQQHVIDWLELSRTDVLCLQELKLPDEKFPRAELAEKGYRSWFAGQKTYNGVGILVRDGVSVDEGSI
VRNIPGFEDPQQRVIAATVEGVRVVCAYFPNGQAPGTDKFAYKLRWLDAMYEWISSELTQHPKLALLGDYNIAPEDRDVH
DPKAWEGQNLVSPEERAQFNRLIELGLVDSFRLFDQPEKTFTWWDYRMLAFRRNAGLRIDHILLSKPLAASCTHCDVDKT
PRKWDQPSDHAPVVALTE
>Mature_258_residues
MKLATWNVNSLKVRQQHVIDWLELSRTDVLCLQELKLPDEKFPRAELAEKGYRSWFAGQKTYNGVGILVRDGVSVDEGSI
VRNIPGFEDPQQRVIAATVEGVRVVCAYFPNGQAPGTDKFAYKLRWLDAMYEWISSELTQHPKLALLGDYNIAPEDRDVH
DPKAWEGQNLVSPEERAQFNRLIELGLVDSFRLFDQPEKTFTWWDYRMLAFRRNAGLRIDHILLSKPLAASCTHCDVDKT
PRKWDQPSDHAPVVALTE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=35.8208955223881, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI221330655, Length=264, Percent_Identity=25.7575757575758, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136678, Length=264, Percent_Identity=25.7575757575758, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29608; Mature: 29608

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATWNVNSLKVRQQHVIDWLELSRTDVLCLQELKLPDEKFPRAELAEKGYRSWFAGQK
CEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
TYNGVGILVRDGVSVDEGSIVRNIPGFEDPQQRVIAATVEGVRVVCAYFPNGQAPGTDKF
CCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCHH
AYKLRWLDAMYEWISSELTQHPKLALLGDYNIAPEDRDVHDPKAWEGQNLVSPEERAQFN
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLIELGLVDSFRLFDQPEKTFTWWDYRMLAFRRNAGLRIDHILLSKPLAASCTHCDVDKT
HHHHHCCCHHHHHHCCCCCCEEEHHHEEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCC
PRKWDQPSDHAPVVALTE
CCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKLATWNVNSLKVRQQHVIDWLELSRTDVLCLQELKLPDEKFPRAELAEKGYRSWFAGQK
CEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
TYNGVGILVRDGVSVDEGSIVRNIPGFEDPQQRVIAATVEGVRVVCAYFPNGQAPGTDKF
CCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCHH
AYKLRWLDAMYEWISSELTQHPKLALLGDYNIAPEDRDVHDPKAWEGQNLVSPEERAQFN
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLIELGLVDSFRLFDQPEKTFTWWDYRMLAFRRNAGLRIDHILLSKPLAASCTHCDVDKT
HHHHHCCCHHHHHHCCCCCCEEEHHHEEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCC
PRKWDQPSDHAPVVALTE
CCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]