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The map label for this gene is hisZ

Identifier: 186476168

GI number: 186476168

Start: 1589227

End: 1590378

Strand: Reverse

Name: hisZ

Synonym: Bphy_1410

Alternate gene names: 186476168

Gene position: 1590378-1589227 (Counterclockwise)

Preceding gene: 186476169

Following gene: 186476167

Centisome position: 45.71

GC content: 66.58

Gene sequence:

>1152_bases
ATGTCGACCTGGTTACTTCCCGAGAATATTGCCGACGTGTTGCCGTCGGAGGCACGCAAGATCGAAGAATTGCGCCGCCG
GCTGCTGGACCGGTTTCGCGCCTATGGCTACGAAATGGTCATGCCGCCGCTGCTCGAATACCTCGAGTCGCTACTGACGG
GCGGCGGCCGCGACCTGAACCTGCGCACCTTCAAGCTCGTCGACCAGCTGTCGGGCCGCACGCTCGGGCTGCGCGCCGAC
ATCACGCCGCAGGTTGCGCGCATCGACGCGCACCTGCTGAACCGTCAGGGCGTGACGCGTCTGTGCTACGCGGGCAACGT
GCTGCACACGCGTCCGCGCGGCCTACATGCGACGCGCGAGCAGATCCAGATCGGTGCCGAAATCTACGGTCATGCGGGTC
TCGAAGCCGACCTGGAAATTCAGCAACTGATGCTCGACGCGCTGCATCTCGCGGGCCTCGGCCGTGTGCGTCTGGACCTG
TGCCATGCCGCCGTGCTGGGTGCGCTGGTCGACAGCGAGCCCGCTGCTGCCGAACTCGGCGAGTCGCTGTATGAAACGCT
CGCGGGCAAGGATGTGCCGCTCTTGACCGAACTGACGGCAAACCTCCCGCCCGTCACGCGCGATGCTCTGCGTGCGCTGC
CCACGCTGTACGGCGACGCGACGGTGCTCGAAGAGGCGCGCGCGCGCTTGCCGAACGCGCCGGAAATCTCACGCGCGCTC
GACGATCTCGCGTTTCTCGCGCAGCAGGTCGGCGGCGCGGAAGTGATGATCGATCTGGCCGATCTGCGCGGCTACGCGTA
TCACAGCGGTGTGATGTTCTCGGCATATGTCGACGGCGTGCCGAATGCAGTCGCGCGCGGCGGTCGTTACGACAAGGTCG
GCCAGGCGTACGGGCGCGCTCGTGCTGCGACGGGCTTTTCGCTGGATCTGCGCGAAGTCGCGCGCATTTCGCCCATCGAG
GCGCGCAGCAGTGCGATCCTCGCACCGTGGCAAGCGGGCGAAGCGTTGCGTACGAGCGTTGCAGCGTTGCGCGATGCCGG
CGAAGTCGTGATCCAGGCGCTGCCGGGTCACGATCACGCGCTCGACGAATTCGCGTGCGACCGCGTGCTGGTCGAGCGCG
ACGGCCAGTGGGTGGTCGAGTTGAAGTCCTAG

Upstream 100 bases:

>100_bases
CGCGCGCCAGCGCCTTTCGTGCGCTCACGCGTATGCGTCGCTCCCATCATTCCCAACGGCGTTGTGATGACGCCGGATTC
ACCGTCGTAGGACTGTATCG

Downstream 100 bases:

>100_bases
TGTGGTGAGTGCGCGCGGCGTCATGCGAAACGTGACGCCGCAACGATCTGAATGCCCGCCCGCCACCGGCCGCAGCGACG
GCCTTTGGACGATGCGGTTC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRAYGYEMVMPPLLEYLESLLTGGGRDLNLRTFKLVDQLSGRTLGLRAD
ITPQVARIDAHLLNRQGVTRLCYAGNVLHTRPRGLHATREQIQIGAEIYGHAGLEADLEIQQLMLDALHLAGLGRVRLDL
CHAAVLGALVDSEPAAAELGESLYETLAGKDVPLLTELTANLPPVTRDALRALPTLYGDATVLEEARARLPNAPEISRAL
DDLAFLAQQVGGAEVMIDLADLRGYAYHSGVMFSAYVDGVPNAVARGGRYDKVGQAYGRARAATGFSLDLREVARISPIE
ARSSAILAPWQAGEALRTSVAALRDAGEVVIQALPGHDHALDEFACDRVLVERDGQWVVELKS

Sequences:

>Translated_383_residues
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRAYGYEMVMPPLLEYLESLLTGGGRDLNLRTFKLVDQLSGRTLGLRAD
ITPQVARIDAHLLNRQGVTRLCYAGNVLHTRPRGLHATREQIQIGAEIYGHAGLEADLEIQQLMLDALHLAGLGRVRLDL
CHAAVLGALVDSEPAAAELGESLYETLAGKDVPLLTELTANLPPVTRDALRALPTLYGDATVLEEARARLPNAPEISRAL
DDLAFLAQQVGGAEVMIDLADLRGYAYHSGVMFSAYVDGVPNAVARGGRYDKVGQAYGRARAATGFSLDLREVARISPIE
ARSSAILAPWQAGEALRTSVAALRDAGEVVIQALPGHDHALDEFACDRVLVERDGQWVVELKS
>Mature_382_residues
STWLLPENIADVLPSEARKIEELRRRLLDRFRAYGYEMVMPPLLEYLESLLTGGGRDLNLRTFKLVDQLSGRTLGLRADI
TPQVARIDAHLLNRQGVTRLCYAGNVLHTRPRGLHATREQIQIGAEIYGHAGLEADLEIQQLMLDALHLAGLGRVRLDLC
HAAVLGALVDSEPAAAELGESLYETLAGKDVPLLTELTANLPPVTRDALRALPTLYGDATVLEEARARLPNAPEISRALD
DLAFLAQQVGGAEVMIDLADLRGYAYHSGVMFSAYVDGVPNAVARGGRYDKVGQAYGRARAATGFSLDLREVARISPIEA
RSSAILAPWQAGEALRTSVAALRDAGEVVIQALPGHDHALDEFACDRVLVERDGQWVVELKS

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_BURP8 (B2JIU1)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857638.1
- GeneID:   6242901
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1410
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 41718; Mature: 41587

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWLLPENIADVLPSEARKIEELRRRLLDRFRAYGYEMVMPPLLEYLESLLTGGGRDLN
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
LRTFKLVDQLSGRTLGLRADITPQVARIDAHLLNRQGVTRLCYAGNVLHTRPRGLHATRE
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCHHHHH
QIQIGAEIYGHAGLEADLEIQQLMLDALHLAGLGRVRLDLCHAAVLGALVDSEPAAAELG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ESLYETLAGKDVPLLTELTANLPPVTRDALRALPTLYGDATVLEEARARLPNAPEISRAL
HHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
DDLAFLAQQVGGAEVMIDLADLRGYAYHSGVMFSAYVDGVPNAVARGGRYDKVGQAYGRA
HHHHHHHHHCCCCEEEEEHHHHCCHHHHCCEEEEHHHCCCCHHHHCCCCCHHHHHHHHHH
RAATGFSLDLREVARISPIEARSSAILAPWQAGEALRTSVAALRDAGEVVIQALPGHDHA
HHCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHH
LDEFACDRVLVERDGQWVVELKS
HHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
STWLLPENIADVLPSEARKIEELRRRLLDRFRAYGYEMVMPPLLEYLESLLTGGGRDLN
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
LRTFKLVDQLSGRTLGLRADITPQVARIDAHLLNRQGVTRLCYAGNVLHTRPRGLHATRE
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCHHHHH
QIQIGAEIYGHAGLEADLEIQQLMLDALHLAGLGRVRLDLCHAAVLGALVDSEPAAAELG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
ESLYETLAGKDVPLLTELTANLPPVTRDALRALPTLYGDATVLEEARARLPNAPEISRAL
HHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHH
DDLAFLAQQVGGAEVMIDLADLRGYAYHSGVMFSAYVDGVPNAVARGGRYDKVGQAYGRA
HHHHHHHHHCCCCEEEEEHHHHCCHHHHCCEEEEHHHCCCCHHHHCCCCCHHHHHHHHHH
RAATGFSLDLREVARISPIEARSSAILAPWQAGEALRTSVAALRDAGEVVIQALPGHDHA
HHCCCCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHH
LDEFACDRVLVERDGQWVVELKS
HHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA