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The map label for this gene is purA

Identifier: 186476167

GI number: 186476167

Start: 1587671

End: 1589017

Strand: Reverse

Name: purA

Synonym: Bphy_1409

Alternate gene names: 186476167

Gene position: 1589017-1587671 (Counterclockwise)

Preceding gene: 186476168

Following gene: 186476166

Centisome position: 45.67

GC content: 62.29

Gene sequence:

>1347_bases
ATGTCTGCCAGCGCAGTGAATGTGAACCCTGGGCGCAATGTCGTCGTCGTGGGTACCCAGTGGGGTGATGAGGGCAAAGG
CAAGATCGTTGACTGGCTCACGGACCACGCGCAAGGCGTCGTTCGTTTCCAGGGCGGTCATAACGCCGGTCATACGCTCA
TCATCGGCGGCAAGAAAACCATCTTGCGTCTGATTCCGTCGGGCATCATGCGCCCGGGCGTCGCCTGCTACATCGGCAAT
GGCGTCGTGTTGTCTCCGGAAGCGCTATTCAAGGAAATCGAAGAGCTGGAATCGGCCGGCATCGACGTGCAAAAGCGTCT
CTTCATTTCCGAAGCCACCACGCTGATTCTCCCGTACCACGTTGCCATCGACCAGGCGCGCGAAGCACGCAGCGGCGCCG
GCAAGATCGGCACGACGGGACGCGGCATCGGCCCGGCCTACGAGGACAAGGTCGCGCGCCGCGGTTTGCGCGTGCAGGAC
CTGTTCGAGCCGAAGGCTTTCGCCGAACGTCTGCGTGCGAACCTCGATTTCCACAACTTCGTGCTCACGCAATATCTGGG
TGCGCCGGCTGTCGACTATCAGCAGACGCTCGACATGATGCTGAGCTATGCCGACCGTCTGAAGCCGATGGTGACGGATG
TGTCACGCCGTCTCTATGACGAGAACCACGTGGGCAACAACCTGCTGTTCGAAGGCGCGCAAGGCACGCTGCTCGATATC
GATCACGGCACGTATCCGTTCGTGACGTCGAGCAATTGCGTCGCGGGTGCGGCGACGGCGGGCGCGGGCATCGGCCCGCA
AAAGCTGGACTACATCCTCGGTATCACAAAGGCGTACTGCACGCGTGTCGGTTCGGGTCCGTTCCCGAGCGAACTGTATG
ACGCGGACAACGCGAACCGGCAGGAAGAGGTCGGCCTGACGCTCGCGAAGGTCGGCAAGGAATTCGGCTCGGTCACGGGT
CGTCCACGCCGCACCGGCTGGCTCGACGCTGCCGCGCTGCGCCGTGCGATCCAGATCAACGGCGTATCGGGTCTTTGCAT
CACGAAGCTCGACGTGCTCGACGGCCTCGACGAAGTGAAGCTGTGCGTCGGGTATACGATCGATGGCAAGGACGCCGACA
TCCTGCCGCGCGGCGCGTATGAAGTGTCGCGCTGCGAGCCGGTGTACGAAACCTTTGGTGGCTGGAAGGAAAGCACCGTC
GGCATCAAGGAGTGGAACAAGCTGCCCGCCAACGCTCAGGCGTATCTGACGCGCGTGCAGGAAGTGGCGGGCGTGCCCAT
CGACATGGTTTCGACGGGTCCGGACCGCGACGAGACCATTCTTCTGCGTCACCCGTTCAAGGTTTAA

Upstream 100 bases:

>100_bases
TTTGAGCGGAAACGGAAATTCTGGACAACAGTCGGCGAAAAAATCCGGAAGCTTCCTCGAACATAGGTAAAATACGTTTT
TAACCAGCTTAAGAAACAAC

Downstream 100 bases:

>100_bases
CGCATGATCCAGGGTGTACCGATGATTGAAATGAAGGATCCGCGCAACGACGAGCGCAATCTGTGGGTCGGCTGGGACGA
ATATCACAGGCTGATCGAGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRPGVACYIGN
GVVLSPEALFKEIEELESAGIDVQKRLFISEATTLILPYHVAIDQAREARSGAGKIGTTGRGIGPAYEDKVARRGLRVQD
LFEPKAFAERLRANLDFHNFVLTQYLGAPAVDYQQTLDMMLSYADRLKPMVTDVSRRLYDENHVGNNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAATAGAGIGPQKLDYILGITKAYCTRVGSGPFPSELYDADNANRQEEVGLTLAKVGKEFGSVTG
RPRRTGWLDAAALRRAIQINGVSGLCITKLDVLDGLDEVKLCVGYTIDGKDADILPRGAYEVSRCEPVYETFGGWKESTV
GIKEWNKLPANAQAYLTRVQEVAGVPIDMVSTGPDRDETILLRHPFKV

Sequences:

>Translated_448_residues
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRPGVACYIGN
GVVLSPEALFKEIEELESAGIDVQKRLFISEATTLILPYHVAIDQAREARSGAGKIGTTGRGIGPAYEDKVARRGLRVQD
LFEPKAFAERLRANLDFHNFVLTQYLGAPAVDYQQTLDMMLSYADRLKPMVTDVSRRLYDENHVGNNLLFEGAQGTLLDI
DHGTYPFVTSSNCVAGAATAGAGIGPQKLDYILGITKAYCTRVGSGPFPSELYDADNANRQEEVGLTLAKVGKEFGSVTG
RPRRTGWLDAAALRRAIQINGVSGLCITKLDVLDGLDEVKLCVGYTIDGKDADILPRGAYEVSRCEPVYETFGGWKESTV
GIKEWNKLPANAQAYLTRVQEVAGVPIDMVSTGPDRDETILLRHPFKV
>Mature_447_residues
SASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRPGVACYIGNG
VVLSPEALFKEIEELESAGIDVQKRLFISEATTLILPYHVAIDQAREARSGAGKIGTTGRGIGPAYEDKVARRGLRVQDL
FEPKAFAERLRANLDFHNFVLTQYLGAPAVDYQQTLDMMLSYADRLKPMVTDVSRRLYDENHVGNNLLFEGAQGTLLDID
HGTYPFVTSSNCVAGAATAGAGIGPQKLDYILGITKAYCTRVGSGPFPSELYDADNANRQEEVGLTLAKVGKEFGSVTGR
PRRTGWLDAAALRRAIQINGVSGLCITKLDVLDGLDEVKLCVGYTIDGKDADILPRGAYEVSRCEPVYETFGGWKESTVG
IKEWNKLPANAQAYLTRVQEVAGVPIDMVSTGPDRDETILLRHPFKV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=437, Percent_Identity=43.0205949656751, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI34577063, Length=443, Percent_Identity=40.4063205417607, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=41.7283950617284, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=61.0983981693364, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=38.7243735763098, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=38.7614678899083, Blast_Score=304, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=40.7322654462243, Blast_Score=319, Evalue=5e-88,
Organism=Drosophila melanogaster, GI21356033, Length=456, Percent_Identity=38.1578947368421, Blast_Score=304, Evalue=1e-82,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BURP8 (B2JIU0)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857637.1
- ProteinModelPortal:   B2JIU0
- SMR:   B2JIU0
- GeneID:   6242900
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1409
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48517; Mature: 48386

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 23-23 ACT_SITE 51-51 BINDING 139-139 BINDING 153-153 BINDING 234-234 BINDING 249-249 BINDING 321-321 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMRPGVACYIGNGVVLSPEALFKEIEELESAGIDVQKRLFISEATTLILPYH
HHHHHHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEHH
VAIDQAREARSGAGKIGTTGRGIGPAYEDKVARRGLRVQDLFEPKAFAERLRANLDFHNF
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHH
VLTQYLGAPAVDYQQTLDMMLSYADRLKPMVTDVSRRLYDENHVGNNLLFEGAQGTLLDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAATAGAGIGPQKLDYILGITKAYCTRVGSGPFPSELYDADNANR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
QEEVGLTLAKVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCITKLDVLDGLDEVK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEHHHHHCCCCCEE
LCVGYTIDGKDADILPRGAYEVSRCEPVYETFGGWKESTVGIKEWNKLPANAQAYLTRVQ
EEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHH
EVAGVPIDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SASAVNVNPGRNVVVVGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKT
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHH
ILRLIPSGIMRPGVACYIGNGVVLSPEALFKEIEELESAGIDVQKRLFISEATTLILPYH
HHHHHHHHHCCCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEHH
VAIDQAREARSGAGKIGTTGRGIGPAYEDKVARRGLRVQDLFEPKAFAERLRANLDFHNF
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHH
VLTQYLGAPAVDYQQTLDMMLSYADRLKPMVTDVSRRLYDENHVGNNLLFEGAQGTLLDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEC
DHGTYPFVTSSNCVAGAATAGAGIGPQKLDYILGITKAYCTRVGSGPFPSELYDADNANR
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
QEEVGLTLAKVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCITKLDVLDGLDEVK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEECCCCCCCEEHHHHHCCCCCEE
LCVGYTIDGKDADILPRGAYEVSRCEPVYETFGGWKESTVGIKEWNKLPANAQAYLTRVQ
EEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHH
EVAGVPIDMVSTGPDRDETILLRHPFKV
HHHCCCEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA