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The map label for this gene is lpxD

Identifier: 186476086

GI number: 186476086

Start: 1486560

End: 1487636

Strand: Reverse

Name: lpxD

Synonym: Bphy_1328

Alternate gene names: 186476086

Gene position: 1487636-1486560 (Counterclockwise)

Preceding gene: 186476087

Following gene: 186476085

Centisome position: 42.76

GC content: 64.81

Gene sequence:

>1077_bases
ATGGCATTCACGCTCGAGGAGATCGTCCAGCGGTTCGGCGGAGAGATCGTCGGCGATCGCGCGCACCGCGTCGGCAATCT
GGCGCCGCTCGATCAGGCTGGCCCCGATCAACTGGCGTTTCTCGCCAATCCGAAGTACCTGGCGCAAGTCGAAACGACGC
GCGCGGGCGCCGTGCTGATCAATGCCGCGGATCTCGCGAAGCTAGCGTCGCCCGACGGTCGCAATTTCATCGTCACGCCC
AATCCGTACGCGTACTTCGCGCGCCTCGCGCAGGCCTTCATCGACATGGCCACGCCGAAGGCCAAGCGGGGCGTGCATCC
GAGCGCAACCGTCGACTCGTCCGCTCAGATCGCGGCAACCGCTGTGATCGGCCCGAACGTGACGGTCGAAGCGGGTGTGG
CGATCGGCGAGAACGTGCGCCTCGACGCGAATGTGTTCGTCGGACGCGGCACGACCATCGGTGCCGGTTCGCACTTGTAT
CCGAACGTCGCCGTGTATCACGGCTGCAGGCTCGGCGAGCGCGCAATCGTCCATGCAGGCGCGGTGATCGGCTCGGACGG
CTTCGGTTTCGCGCCGGACTTCGTCGGCGACGGTGACGCGCGCACGGGCAGCTGGGTGAAAATTCCGCAGGTCGGCGGCG
TATCGATCGGGCCGGATGTCGAAATCGGCGCAAATACCACGATCGACCGCGGCGCGATGGCCGATACTGTCATCGAAGAA
TGCGTGAAGATCGATAACCTCGTGCAAATCGGCCATAACTGCAAGATCGGCGCCTACACGGTCATCGCCGGTTGTGCCGG
CATTGCGGGCAGCACGACGATCGGCCGTCACTGCATGATCGGCGGCGCCGTCGGCATTGCCGGCCACGTCACTTTGGGCG
ACTACGTGATCGTCACGGCGAAGTCGGGTGTGTCGAAATCGCTTCCGAAAGCCGGGATCTACACGAGCGCATTCCCGGCC
GTGGACCATGCCGACTGGAACAGGAGCGCCGCGTTGATGCGCAACCTCGACAAGTTGCGCGATCGCATCAAGGCGCTCGA
AGCCGCGGTCGCCAGTGCCTCCGGAGACAAGGCGTAA

Upstream 100 bases:

>100_bases
AGCCGGGCCGGACGGCGCCTGCATCGGCCGTCTGGTGCAACACCTCCGGCGCGCGACATCCGCGGCGCTTGGGTAACTGA
ACAGCAGGAGCAGTAGACGC

Downstream 100 bases:

>100_bases
GCACCTCGGCACCATCAGCACGACGGGAAAAAAAGCGCTGGCGCGTGTGGCCGACAGGCCGCACGCAGCCAGCACAGGAA
CGGGCGGCAGGGCGCGTCGT

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MAFTLEEIVQRFGGEIVGDRAHRVGNLAPLDQAGPDQLAFLANPKYLAQVETTRAGAVLINAADLAKLASPDGRNFIVTP
NPYAYFARLAQAFIDMATPKAKRGVHPSATVDSSAQIAATAVIGPNVTVEAGVAIGENVRLDANVFVGRGTTIGAGSHLY
PNVAVYHGCRLGERAIVHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVSIGPDVEIGANTTIDRGAMADTVIEE
CVKIDNLVQIGHNCKIGAYTVIAGCAGIAGSTTIGRHCMIGGAVGIAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFPA
VDHADWNRSAALMRNLDKLRDRIKALEAAVASASGDKA

Sequences:

>Translated_358_residues
MAFTLEEIVQRFGGEIVGDRAHRVGNLAPLDQAGPDQLAFLANPKYLAQVETTRAGAVLINAADLAKLASPDGRNFIVTP
NPYAYFARLAQAFIDMATPKAKRGVHPSATVDSSAQIAATAVIGPNVTVEAGVAIGENVRLDANVFVGRGTTIGAGSHLY
PNVAVYHGCRLGERAIVHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVSIGPDVEIGANTTIDRGAMADTVIEE
CVKIDNLVQIGHNCKIGAYTVIAGCAGIAGSTTIGRHCMIGGAVGIAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFPA
VDHADWNRSAALMRNLDKLRDRIKALEAAVASASGDKA
>Mature_357_residues
AFTLEEIVQRFGGEIVGDRAHRVGNLAPLDQAGPDQLAFLANPKYLAQVETTRAGAVLINAADLAKLASPDGRNFIVTPN
PYAYFARLAQAFIDMATPKAKRGVHPSATVDSSAQIAATAVIGPNVTVEAGVAIGENVRLDANVFVGRGTTIGAGSHLYP
NVAVYHGCRLGERAIVHAGAVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVSIGPDVEIGANTTIDRGAMADTVIEEC
VKIDNLVQIGHNCKIGAYTVIAGCAGIAGSTTIGRHCMIGGAVGIAGHVTLGDYVIVTAKSGVSKSLPKAGIYTSAFPAV
DHADWNRSAALMRNLDKLRDRIKALEAAVASASGDKA

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=351, Percent_Identity=40.7407407407407, Blast_Score=257, Evalue=8e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_BURP8 (B2JIB6)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857556.1
- ProteinModelPortal:   B2JIB6
- GeneID:   6242819
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1328
- HOGENOM:   HBG469615
- OMA:   CFVGKNT
- ProtClustDB:   PRK00892
- HAMAP:   MF_00523
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36867; Mature: 36736

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTLEEIVQRFGGEIVGDRAHRVGNLAPLDQAGPDQLAFLANPKYLAQVETTRAGAVLI
CCCCHHHHHHHHCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEE
NAADLAKLASPDGRNFIVTPNPYAYFARLAQAFIDMATPKAKRGVHPSATVDSSAQIAAT
EHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEE
AVIGPNVTVEAGVAIGENVRLDANVFVGRGTTIGAGSHLYPNVAVYHGCRLGERAIVHAG
EEECCCCEEEECEEECCCEEEECEEEEECCCEECCCCCCCCCCCEEECCCCCCHHEEEEC
AVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVSIGPDVEIGANTTIDRGAMADTVIEE
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHH
CVKIDNLVQIGHNCKIGAYTVIAGCAGIAGSTTIGRHCMIGGAVGIAGHVTLGDYVIVTA
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECCEEEEEE
KSGVSKSLPKAGIYTSAFPAVDHADWNRSAALMRNLDKLRDRIKALEAAVASASGDKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AFTLEEIVQRFGGEIVGDRAHRVGNLAPLDQAGPDQLAFLANPKYLAQVETTRAGAVLI
CCCHHHHHHHHCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCEEEE
NAADLAKLASPDGRNFIVTPNPYAYFARLAQAFIDMATPKAKRGVHPSATVDSSAQIAAT
EHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEE
AVIGPNVTVEAGVAIGENVRLDANVFVGRGTTIGAGSHLYPNVAVYHGCRLGERAIVHAG
EEECCCCEEEECEEECCCEEEECEEEEECCCEECCCCCCCCCCCEEECCCCCCHHEEEEC
AVIGSDGFGFAPDFVGDGDARTGSWVKIPQVGGVSIGPDVEIGANTTIDRGAMADTVIEE
EEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHH
CVKIDNLVQIGHNCKIGAYTVIAGCAGIAGSTTIGRHCMIGGAVGIAGHVTLGDYVIVTA
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECCEEEEEE
KSGVSKSLPKAGIYTSAFPAVDHADWNRSAALMRNLDKLRDRIKALEAAVASASGDKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA