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The map label for this gene is 186475870

Identifier: 186475870

GI number: 186475870

Start: 1249721

End: 1251085

Strand: Direct

Name: 186475870

Synonym: Bphy_1105

Alternate gene names: NA

Gene position: 1249721-1251085 (Clockwise)

Preceding gene: 186475867

Following gene: 186475874

Centisome position: 35.92

GC content: 62.05

Gene sequence:

>1365_bases
ATGAAACTGACCTTTCTTGGTGCGAGCGAGACCGTCACCGGTTCGAAATATCTGCTCGAGGAATCCGGCATCCGCATCCT
GATCGATTGCGGGCTGTTTCAGGGTGTAAAGAACTTGCGGATGCGCAACTGGATGCCGCTGCCTGTGGCCGCCGAGTCAA
TCGATACGGTGATACTCACGCATGCGCACATCGATCACAGCGGCTACTTGCCACTACTCGTGCGCAATGGTTTCAACGGC
CGCGTGCACTGCACGCCAGGCACCGCCGCGCTCTGCGAGATCATGCTGCGCGACAGCGCACGCCTGCAGGAAGAGGAAGC
GGATTTTGCGAACCGGCACGGTTTCTCGAAGCATCATCCCGCGCTGCCCCTCTACACGATGCAGGATGCAGAGCGTGCGC
TGCGACTGATCGTGCCATGCACTTTCGACCATCCCGTCGACCTCGGTCACGGACTGAGCTTTCGCTTTTTGCCCGCCGGT
CACATCCTCGGCGCGGCGAGCGTCGTGATCTGCCGTCATCACAAGGTGCTCGCGTTCTCGGGCGATGTGGGGCGGCCGAA
TGACCCCATCATGCGCCCACCCGCACCGCTGGCGCACGCGGACTACCTGATAGTCGAGTCGACTTACGGCGACCGCTTGC
ACCCCGAGTCGAATCCGGAGGACGAGCTTGCGCAGATATTCACCAAGACGTTCGCGAAAGGCGGCGTTGTCGTGATGCCT
TGCTTCGCGGTCGGGCGCGCACAGGAGATTCTCTACTACATCGCGAAACTCAAGGAGACCAGACGCATGGCGAACGTTCC
CGTCTACCTGGACAGCCCGATGGCGACGAACGTCACGGAGGTCTACCGCAAACACCTCGACGAACATCGATTGACCATCT
CCGAGGCACAGGCAATCGACAAGGTGGCGATCATGACCCGGAGCGTCGATCAATCGAAGGCGATCGCGTCGCATCACGGT
CCCATGCTGATCATCGCGGGCAGTGGCATGGCGACGGGCGGCCGTGTGCTGCATCATCTGCGTCTTTACGCGCCGGATCC
TCGCAACACGATCGCGCTGGTGGGCTTTCAGGCGGCGGGCACGCGCGGCGCCGCGCTCGCCGCTCACGAGAGTTCGATCA
AGTTGCACGGCGAGTATGTACGCGTGCGGGCAAACGTCGAGTCGATCGCGTCGCTCTCGGCTCACGCCGACTACCGCGAA
CTGCTGCAATGGCTGTCGCATCTGACCGACCCGCCCGTGCGGACTTTCGTCACACATGGCGAGCCGGCGGCGGCAGACGC
AATGCGGCGTCGAATCGCCGAATCACTCGGGTGGTCGTGCGAGGTTCCCGTCTACCAACAGACGGTTGAACTCGAATCGC
TTTGA

Upstream 100 bases:

>100_bases
GTCTCGCCGCCACGACGACCGACAGGCGAATGATCTAGGTCAATAAGCTCGGACTCGAATGAGATGCAATTGACAACACG
TCCGCATGCATGGAGACCTC

Downstream 100 bases:

>100_bases
CGTGCGCCGCGTTTGAGTTGACGTCGCGCCCGGTGCAGCCGCGCGGCTACCGCTCGAACACGTAGGTGCCCGGCGCGTCT
TCCAGTCCGTCGACGATCGC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKLTFLGASETVTGSKYLLEESGIRILIDCGLFQGVKNLRMRNWMPLPVAAESIDTVILTHAHIDHSGYLPLLVRNGFNG
RVHCTPGTAALCEIMLRDSARLQEEEADFANRHGFSKHHPALPLYTMQDAERALRLIVPCTFDHPVDLGHGLSFRFLPAG
HILGAASVVICRHHKVLAFSGDVGRPNDPIMRPPAPLAHADYLIVESTYGDRLHPESNPEDELAQIFTKTFAKGGVVVMP
CFAVGRAQEILYYIAKLKETRRMANVPVYLDSPMATNVTEVYRKHLDEHRLTISEAQAIDKVAIMTRSVDQSKAIASHHG
PMLIIAGSGMATGGRVLHHLRLYAPDPRNTIALVGFQAAGTRGAALAAHESSIKLHGEYVRVRANVESIASLSAHADYRE
LLQWLSHLTDPPVRTFVTHGEPAAADAMRRRIAESLGWSCEVPVYQQTVELESL

Sequences:

>Translated_454_residues
MKLTFLGASETVTGSKYLLEESGIRILIDCGLFQGVKNLRMRNWMPLPVAAESIDTVILTHAHIDHSGYLPLLVRNGFNG
RVHCTPGTAALCEIMLRDSARLQEEEADFANRHGFSKHHPALPLYTMQDAERALRLIVPCTFDHPVDLGHGLSFRFLPAG
HILGAASVVICRHHKVLAFSGDVGRPNDPIMRPPAPLAHADYLIVESTYGDRLHPESNPEDELAQIFTKTFAKGGVVVMP
CFAVGRAQEILYYIAKLKETRRMANVPVYLDSPMATNVTEVYRKHLDEHRLTISEAQAIDKVAIMTRSVDQSKAIASHHG
PMLIIAGSGMATGGRVLHHLRLYAPDPRNTIALVGFQAAGTRGAALAAHESSIKLHGEYVRVRANVESIASLSAHADYRE
LLQWLSHLTDPPVRTFVTHGEPAAADAMRRRIAESLGWSCEVPVYQQTVELESL
>Mature_454_residues
MKLTFLGASETVTGSKYLLEESGIRILIDCGLFQGVKNLRMRNWMPLPVAAESIDTVILTHAHIDHSGYLPLLVRNGFNG
RVHCTPGTAALCEIMLRDSARLQEEEADFANRHGFSKHHPALPLYTMQDAERALRLIVPCTFDHPVDLGHGLSFRFLPAG
HILGAASVVICRHHKVLAFSGDVGRPNDPIMRPPAPLAHADYLIVESTYGDRLHPESNPEDELAQIFTKTFAKGGVVVMP
CFAVGRAQEILYYIAKLKETRRMANVPVYLDSPMATNVTEVYRKHLDEHRLTISEAQAIDKVAIMTRSVDQSKAIASHHG
PMLIIAGSGMATGGRVLHHLRLYAPDPRNTIALVGFQAAGTRGAALAAHESSIKLHGEYVRVRANVESIASLSAHADYRE
LLQWLSHLTDPPVRTFVTHGEPAAADAMRRRIAESLGWSCEVPVYQQTVELESL

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI7706427, Length=426, Percent_Identity=28.6384976525822, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI33300633, Length=459, Percent_Identity=25.2723311546841, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI223555970, Length=414, Percent_Identity=20.5314009661836, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI32564696, Length=465, Percent_Identity=26.6666666666667, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI32566029, Length=429, Percent_Identity=27.972027972028, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6323307, Length=441, Percent_Identity=27.2108843537415, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24648013, Length=423, Percent_Identity=29.0780141843972, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI21358523, Length=462, Percent_Identity=25.7575757575758, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 50004; Mature: 50004

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTFLGASETVTGSKYLLEESGIRILIDCGLFQGVKNLRMRNWMPLPVAAESIDTVILT
CEEEEECCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCHHCCCEEEEE
HAHIDHSGYLPLLVRNGFNGRVHCTPGTAALCEIMLRDSARLQEEEADFANRHGFSKHHP
EEEECCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
ALPLYTMQDAERALRLIVPCTFDHPVDLGHGLSFRFLPAGHILGAASVVICRHHKVLAFS
CCCEEEHHHHHCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHEEEEEECCEEEEEE
GDVGRPNDPIMRPPAPLAHADYLIVESTYGDRLHPESNPEDELAQIFTKTFAKGGVVVMP
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
CFAVGRAQEILYYIAKLKETRRMANVPVYLDSPMATNVTEVYRKHLDEHRLTISEAQAID
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCEEHHHHHHHH
KVAIMTRSVDQSKAIASHHGPMLIIAGSGMATGGRVLHHLRLYAPDPRNTIALVGFQAAG
HHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCHHHHHEEEEECCCCCCEEEEEEEECCC
TRGAALAAHESSIKLHGEYVRVRANVESIASLSAHADYRELLQWLSHLTDPPVRTFVTHG
CCCCEEEECCCCEEEECEEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCHHEEEECC
EPAAADAMRRRIAESLGWSCEVPVYQQTVELESL
CCHHHHHHHHHHHHHCCCEECCCHHHHCEECCCC
>Mature Secondary Structure
MKLTFLGASETVTGSKYLLEESGIRILIDCGLFQGVKNLRMRNWMPLPVAAESIDTVILT
CEEEEECCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCCHHCCCEEEEE
HAHIDHSGYLPLLVRNGFNGRVHCTPGTAALCEIMLRDSARLQEEEADFANRHGFSKHHP
EEEECCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
ALPLYTMQDAERALRLIVPCTFDHPVDLGHGLSFRFLPAGHILGAASVVICRHHKVLAFS
CCCEEEHHHHHCEEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHEEEEEECCEEEEEE
GDVGRPNDPIMRPPAPLAHADYLIVESTYGDRLHPESNPEDELAQIFTKTFAKGGVVVMP
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
CFAVGRAQEILYYIAKLKETRRMANVPVYLDSPMATNVTEVYRKHLDEHRLTISEAQAID
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCEEHHHHHHHH
KVAIMTRSVDQSKAIASHHGPMLIIAGSGMATGGRVLHHLRLYAPDPRNTIALVGFQAAG
HHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCHHHHHEEEEECCCCCCEEEEEEEECCC
TRGAALAAHESSIKLHGEYVRVRANVESIASLSAHADYRELLQWLSHLTDPPVRTFVTHG
CCCCEEEECCCCEEEECEEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCHHEEEECC
EPAAADAMRRRIAESLGWSCEVPVYQQTVELESL
CCHHHHHHHHHHHHHCCCEECCCHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA