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The map label for this gene is pfkB [H]

Identifier: 186475863

GI number: 186475863

Start: 1240373

End: 1241377

Strand: Reverse

Name: pfkB [H]

Synonym: Bphy_1098

Alternate gene names: 186475863

Gene position: 1241377-1240373 (Counterclockwise)

Preceding gene: 186475864

Following gene: 186475859

Centisome position: 35.68

GC content: 64.38

Gene sequence:

>1005_bases
ATGATCGACGTTCTCACAGTCACCCTGAATCCCGCGGTCGATCTATCGACCTCTGTTGAGCGCATCGTGGATACGCACAA
GCTGCGATGCGAAACCGCGCAGCGCGATCCGGGCGGCGGGGGCGTCAACGTTGCGCGGGTGCTGCATCGCCTCGGCAGCG
AATGCGTTGCGCTGTTTGCCGCGGGCGGAGTGACGGGCCGCATGCTTGGCGGCATGCTCGATGGGGAGAAACTTCGCACT
CGCTGTATCGACATCAAAGGCGAGACGCGAGAAAACTTCTCCGTGATGGAAACGTCGACCCGGCGTGAGTACCGATTCGT
GTTGCCTGGGCCAACGCTCGCGCCAGCGGAGTGGACGGAATGTCTGCGCAGCGTGCAGGCGCATGTCGATTCCGCGCGCT
TTCTCGTCCTGAGCGGAAGCCTGCCTCCCGGCGCACCGGCGGACGCCTACGCTCAGCTCGCGCGTGCGGCCGCCACTGCA
CGGCCGAACATTCGCGTGGCCGTGGATGCGTCGGGGCCCACGCTCGCGGCGGCCCTCGCTGGCGGGCTCGTCGATATCGT
CAAGCCGAGTCTGAATGAACTACGCGAATTGACGGCACTGCCGCTGAATGACGTGGGGGACCAGATCGACGCCGCAAGAA
TGTTGATCGTGCAACGCAAGGCGAGGATGGTTGCACTCACGCTTGCTGAGCAAGGCGCGATGCTCGTGACGCACGACGAA
GCGTGGTTCATGCCTGCGCTGAAAACGGAAGTCCTCAGTGCAATCGGCGCGGGCGACAGCTTCCTCGCGGGATTGATCTG
GTCACTCGACCATGGAGCCGTCGCCAGCGAGGCGCTCGCCTATGCGGCCGCAGCCGCGGCGGCGGCGATGAAGCAAACAG
GTACGCGGCTTTGCGATGCACGAGACGTGACGCAGGCTTATGCAAAGGGCGAACGTGCTGTACGGGTACAGGATCCCGCG
CGCTTTCGCATCGTGCATCGCGAGTCGGCACCGTCAAGGCAGTGA

Upstream 100 bases:

>100_bases
GCAGCACGTCGCGCAAGCGGAACGGGAAGCGGCGCATCGTGCGACGGGCGAGCAGACGCGCGGATCGATACTGTCTCAAA
CGGTGCGGTAGGGCCTTGTC

Downstream 100 bases:

>100_bases
AAAATCCCATCAACCGAGTCCAAATCGCCGGACAAACCACGTCTTGGTCAAATGCGTCAAAGCCGCGTAGCTGACCAGGA
TCAGCAGCAGGGCGGGCCAA

Product: 1-phosphofructokinase

Products: ADP; D-Fructose 1,6-bisphosphate; H+ [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIDVLTVTLNPAVDLSTSVERIVDTHKLRCETAQRDPGGGGVNVARVLHRLGSECVALFAAGGVTGRMLGGMLDGEKLRT
RCIDIKGETRENFSVMETSTRREYRFVLPGPTLAPAEWTECLRSVQAHVDSARFLVLSGSLPPGAPADAYAQLARAAATA
RPNIRVAVDASGPTLAAALAGGLVDIVKPSLNELRELTALPLNDVGDQIDAARMLIVQRKARMVALTLAEQGAMLVTHDE
AWFMPALKTEVLSAIGAGDSFLAGLIWSLDHGAVASEALAYAAAAAAAAMKQTGTRLCDARDVTQAYAKGERAVRVQDPA
RFRIVHRESAPSRQ

Sequences:

>Translated_334_residues
MIDVLTVTLNPAVDLSTSVERIVDTHKLRCETAQRDPGGGGVNVARVLHRLGSECVALFAAGGVTGRMLGGMLDGEKLRT
RCIDIKGETRENFSVMETSTRREYRFVLPGPTLAPAEWTECLRSVQAHVDSARFLVLSGSLPPGAPADAYAQLARAAATA
RPNIRVAVDASGPTLAAALAGGLVDIVKPSLNELRELTALPLNDVGDQIDAARMLIVQRKARMVALTLAEQGAMLVTHDE
AWFMPALKTEVLSAIGAGDSFLAGLIWSLDHGAVASEALAYAAAAAAAAMKQTGTRLCDARDVTQAYAKGERAVRVQDPA
RFRIVHRESAPSRQ
>Mature_334_residues
MIDVLTVTLNPAVDLSTSVERIVDTHKLRCETAQRDPGGGGVNVARVLHRLGSECVALFAAGGVTGRMLGGMLDGEKLRT
RCIDIKGETRENFSVMETSTRREYRFVLPGPTLAPAEWTECLRSVQAHVDSARFLVLSGSLPPGAPADAYAQLARAAATA
RPNIRVAVDASGPTLAAALAGGLVDIVKPSLNELRELTALPLNDVGDQIDAARMLIVQRKARMVALTLAEQGAMLVTHDE
AWFMPALKTEVLSAIGAGDSFLAGLIWSLDHGAVASEALAYAAAAAAAAMKQTGTRLCDARDVTQAYAKGERAVRVQDPA
RFRIVHRESAPSRQ

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=310, Percent_Identity=36.7741935483871, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1788493, Length=270, Percent_Identity=28.8888888888889, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11 [C]

Molecular weight: Translated: 35457; Mature: 35457

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVLTVTLNPAVDLSTSVERIVDTHKLRCETAQRDPGGGGVNVARVLHRLGSECVALFA
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGGVTGRMLGGMLDGEKLRTRCIDIKGETRENFSVMETSTRREYRFVLPGPTLAPAEWTE
CCCCHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCEECCCCCCEEEECCCCCCCHHHHHH
CLRSVQAHVDSARFLVLSGSLPPGAPADAYAQLARAAATARPNIRVAVDASGPTLAAALA
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
GGLVDIVKPSLNELRELTALPLNDVGDQIDAARMLIVQRKARMVALTLAEQGAMLVTHDE
CCHHHHHCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECC
AWFMPALKTEVLSAIGAGDSFLAGLIWSLDHGAVASEALAYAAAAAAAAMKQTGTRLCDA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
RDVTQAYAKGERAVRVQDPARFRIVHRESAPSRQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MIDVLTVTLNPAVDLSTSVERIVDTHKLRCETAQRDPGGGGVNVARVLHRLGSECVALFA
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGGVTGRMLGGMLDGEKLRTRCIDIKGETRENFSVMETSTRREYRFVLPGPTLAPAEWTE
CCCCHHHHHHCCCCCHHHHHHEEECCCCCCCCCCCEECCCCCCEEEECCCCCCCHHHHHH
CLRSVQAHVDSARFLVLSGSLPPGAPADAYAQLARAAATARPNIRVAVDASGPTLAAALA
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
GGLVDIVKPSLNELRELTALPLNDVGDQIDAARMLIVQRKARMVALTLAEQGAMLVTHDE
CCHHHHHCCCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEECC
AWFMPALKTEVLSAIGAGDSFLAGLIWSLDHGAVASEALAYAAAAAAAAMKQTGTRLCDA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
RDVTQAYAKGERAVRVQDPARFRIVHRESAPSRQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-Fructose 6-phosphate [C]

Specific reaction: ATP + D-Fructose 6-phosphate --> ADP + D-Fructose 1,6-bisphosphate + H+ [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]