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The map label for this gene is pcaU [H]

Identifier: 186475844

GI number: 186475844

Start: 1215161

End: 1215931

Strand: Reverse

Name: pcaU [H]

Synonym: Bphy_1079

Alternate gene names: 186475844

Gene position: 1215931-1215161 (Counterclockwise)

Preceding gene: 186475850

Following gene: 186475843

Centisome position: 34.95

GC content: 64.59

Gene sequence:

>771_bases
ATGAACCGACCGCCATTGGACAAACGCGACTGGATCGCGGGTCTCGAAAAAGGGCTCGCGATCCTCGAGTCGTTCGACAG
CGAACACGCGCGCCTCACGCCGAGCCAGGCCGCGCAGCTCACGGGCATGACTCGCACGGCTGCGCGCCGCTATCTGCTGA
CGCTCGAGCATCTCGGCTACGTGCAAGGCGACGGCAAGCTCTACGGGCTTACGCCGCGGGTGCTGCGCGTCGGCTGGTCG
TATTTCGATTCGGCGCGTCTGCCGAAGACCGTTCAGCCCTATCTGCAGCAACTGAGCGCAACGATCGGCGAATCCGTCTA
TGTAAGCGTGCTCGACGACTGGGAACTGGTGTTCATCGCGCGTAACGGCACCTCCCGCGTGATGACGACGGGCTTCGTGC
TCGGCGCGCGTGTGCCCGCTCCGCTCACCTCGCCCGGCGTCGTGCTGCTCGCCTATCACCGTAATCAGGAGGCAATGCAG
GCCTGGCTCAACGATACGTCGCTCGCACCGTTCACGCCGCATACGCTGACCAACAGAACAGGTCTGCTCGAAAAGATCCG
CCGCGCGCAGGCGGACGGTTACGCGGTGATCGAGCAGCAACTCGATATCGGCGTGCGCGGCGTCGCGGTGCCGATGAAGA
ACCGTCATGGCGAGGTGGTCGCCGCGCTCAGCACCAACATGCCGATGGGCAAGGAGACGACGGAAGCGGCCCTCACGCGC
GTGTTGCGGCCGCTGCAGGAGTCGGCACTTTCGATGCTCAACGTCCTATAG

Upstream 100 bases:

>100_bases
ACGCGCCGCGCCGCGTGCAACTCGCGGGACGGCACGGCACTGCGTGAGCGATAATCGCGCATATCGATACGTTATCCGCG
CAGATCAGAGGGTGATTGCC

Downstream 100 bases:

>100_bases
TGAAGTGCAGGGATAGCGACGTGGGAGCGAGTCATGCATCGCCCGTTTGCGGATCGCGCTGACGCGGGCCGCGCGCTCGC
GGCACATCTGCAGCACTACG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNRPPLDKRDWIAGLEKGLAILESFDSEHARLTPSQAAQLTGMTRTAARRYLLTLEHLGYVQGDGKLYGLTPRVLRVGWS
YFDSARLPKTVQPYLQQLSATIGESVYVSVLDDWELVFIARNGTSRVMTTGFVLGARVPAPLTSPGVVLLAYHRNQEAMQ
AWLNDTSLAPFTPHTLTNRTGLLEKIRRAQADGYAVIEQQLDIGVRGVAVPMKNRHGEVVAALSTNMPMGKETTEAALTR
VLRPLQESALSMLNVL

Sequences:

>Translated_256_residues
MNRPPLDKRDWIAGLEKGLAILESFDSEHARLTPSQAAQLTGMTRTAARRYLLTLEHLGYVQGDGKLYGLTPRVLRVGWS
YFDSARLPKTVQPYLQQLSATIGESVYVSVLDDWELVFIARNGTSRVMTTGFVLGARVPAPLTSPGVVLLAYHRNQEAMQ
AWLNDTSLAPFTPHTLTNRTGLLEKIRRAQADGYAVIEQQLDIGVRGVAVPMKNRHGEVVAALSTNMPMGKETTEAALTR
VLRPLQESALSMLNVL
>Mature_256_residues
MNRPPLDKRDWIAGLEKGLAILESFDSEHARLTPSQAAQLTGMTRTAARRYLLTLEHLGYVQGDGKLYGLTPRVLRVGWS
YFDSARLPKTVQPYLQQLSATIGESVYVSVLDDWELVFIARNGTSRVMTTGFVLGARVPAPLTSPGVVLLAYHRNQEAMQ
AWLNDTSLAPFTPHTLTNRTGLLEKIRRAQADGYAVIEQQLDIGVRGVAVPMKNRHGEVVAALSTNMPMGKETTEAALTR
VLRPLQESALSMLNVL

Specific function: Activates transcription of the pca operon [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=27.8225806451613, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788131, Length=231, Percent_Identity=26.4069264069264, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082359, Length=228, Percent_Identity=25.8771929824561, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786715, Length=223, Percent_Identity=26.0089686098655, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789999, Length=246, Percent_Identity=22.7642276422764, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28238; Mature: 28238

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPPLDKRDWIAGLEKGLAILESFDSEHARLTPSQAAQLTGMTRTAARRYLLTLEHLGY
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VQGDGKLYGLTPRVLRVGWSYFDSARLPKTVQPYLQQLSATIGESVYVSVLDDWELVFIA
EECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEE
RNGTSRVMTTGFVLGARVPAPLTSPGVVLLAYHRNQEAMQAWLNDTSLAPFTPHTLTNRT
ECCCCCEEHHHEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCHHCCHH
GLLEKIRRAQADGYAVIEQQLDIGVRGVAVPMKNRHGEVVAALSTNMPMGKETTEAALTR
HHHHHHHHHCCCCCEEHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHH
VLRPLQESALSMLNVL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRPPLDKRDWIAGLEKGLAILESFDSEHARLTPSQAAQLTGMTRTAARRYLLTLEHLGY
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VQGDGKLYGLTPRVLRVGWSYFDSARLPKTVQPYLQQLSATIGESVYVSVLDDWELVFIA
EECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEE
RNGTSRVMTTGFVLGARVPAPLTSPGVVLLAYHRNQEAMQAWLNDTSLAPFTPHTLTNRT
ECCCCCEEHHHEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCHHCCHH
GLLEKIRRAQADGYAVIEQQLDIGVRGVAVPMKNRHGEVVAALSTNMPMGKETTEAALTR
HHHHHHHHHCCCCCEEHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHH
VLRPLQESALSMLNVL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515921 [H]