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The map label for this gene is tig

Identifier: 186475775

GI number: 186475775

Start: 1132151

End: 1133497

Strand: Direct

Name: tig

Synonym: Bphy_1010

Alternate gene names: 186475775

Gene position: 1132151-1133497 (Clockwise)

Preceding gene: 186475770

Following gene: 186475776

Centisome position: 32.54

GC content: 61.92

Gene sequence:

>1347_bases
ATGGCTAACGTTGTTGAAAACCTCGGCAAGCTCGAACGCCGCGTGACGATTTCCCTGCCGAAGGACGCCGTGCAGAAGGA
AGTGGACTCGCGTATCCGCCAACTCGCGAAGAACGTGCGCATGCCGGGCTTCCGCCCGGGCAAGGTTCCGCTCAAGATGG
TGACGCAGCAGTATGCGGGTCAGGTGGAAGCCGAAGTGCTGAGCGACAAGGTTGGCAAGCAATTCTTCGATATCAGCCGC
GCGGAGAACCTGCGCGTGGCTGGCCAGCCGAGCTTCGCCCCGAAGGCGGACGCCGTTGAAGGCGACTACGCATTCGACGC
GACATTCGAGGTTTACCCGGAGGTGAAGCTGGGCGACGTCGCCACGGCTGAGATCGAACGCACGAAGACCACCATCAGCG
ACGCGGAAATCGACCGCACGCTGGACATCCTGCGCAAGCAGCGGGTGCACTACCACGCTCGCGGCGAAGCGGGCGAGCAC
GGCGACGGCGGTGCGGATACGGCAGCCAAGGACGGCGATCGCGTGACGGTCGACTTCGTCGGCAAGATCGACGGTGAAGC
ATTCCAGGGCGGCACCGCAGACGACTTCGCGTTTGTCCTCGGCGAAGGCCGCATGCTGCCGGAATTCGAAAAGGCAGCGC
TTGGCCTAAAGGTCGGCGAGTCGAAGGAGTTCGACCTTGCCTTCCCGGAGGACTATCACGGCAAGGAAGTCGCCGGCAAG
ACCGCGCAGTTCGCGATCACGATGAAGAAGATCGAGTGGCCGCACCTTCCCGAGATCGACGCTGAATTCGCGAAGTCGCT
CGGCATCGAAGACGGTGACCTGACGAAGATGCGCAATGAAATCAGGGACAACCTCGAGCGCGAAGCGAAGCGCCGCACGC
AGGCTGTCGTGAAGAACCAGGTAATGGACGCGCTCCTGAAGATCTCGGAACTCGACGTGCCGAAGGCGCTGATCGAGCAG
GACCAGCAGCGACTCGTCGAAATGGCGCGTCAGGATCTGGTGCAGCGCGGCGTACCGAACGCCAAGGACGCACCAATCCC
CGCAGAAATGTTCGCCGAGCAGGCCGAGCGCCGCGTCAAGCTGGGCCTGGTGCTGGCGGAACTGGTGAAGGCCAACGAAC
TGCAGGCGAAGCCGGAGCAGATCCGCGCGGAAGTCGACGAATTCGCGAAGAGCTACGAAGACCCGAAGGAAGTCGTGCGC
TGGTATTATTCGAACCAGCAGCGCCTGGCCGAGATGGAAGCGTATGTCGTTGAATCCAACGTCGTCGATTTCGTCCTGAG
CAAGGCAAAGGTGACGGACAAGGAAGTGAGCTTCGAAGAGCTGGCAAGCGCAACGGCGCAAGCGTAA

Upstream 100 bases:

>100_bases
ATCAACACGCGGCGTCGGGCGCGCTACGCAAAGCGGACATCGACAGGCGGCGCAGACAAGATAACTGATTCGCTCGAATA
ATTTTAGGACGATTGAAGCC

Downstream 100 bases:

>100_bases
TAGCATCGCTGCAACGGCGTGCCGGCTGTCCGAACGACGGCCCGCACGCCGTTTTTGCATCTGCGCACGGTCTTCATGCT
AAAACAGCGCGTGTCGACCG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MANVVENLGKLERRVTISLPKDAVQKEVDSRIRQLAKNVRMPGFRPGKVPLKMVTQQYAGQVEAEVLSDKVGKQFFDISR
AENLRVAGQPSFAPKADAVEGDYAFDATFEVYPEVKLGDVATAEIERTKTTISDAEIDRTLDILRKQRVHYHARGEAGEH
GDGGADTAAKDGDRVTVDFVGKIDGEAFQGGTADDFAFVLGEGRMLPEFEKAALGLKVGESKEFDLAFPEDYHGKEVAGK
TAQFAITMKKIEWPHLPEIDAEFAKSLGIEDGDLTKMRNEIRDNLEREAKRRTQAVVKNQVMDALLKISELDVPKALIEQ
DQQRLVEMARQDLVQRGVPNAKDAPIPAEMFAEQAERRVKLGLVLAELVKANELQAKPEQIRAEVDEFAKSYEDPKEVVR
WYYSNQQRLAEMEAYVVESNVVDFVLSKAKVTDKEVSFEELASATAQA

Sequences:

>Translated_448_residues
MANVVENLGKLERRVTISLPKDAVQKEVDSRIRQLAKNVRMPGFRPGKVPLKMVTQQYAGQVEAEVLSDKVGKQFFDISR
AENLRVAGQPSFAPKADAVEGDYAFDATFEVYPEVKLGDVATAEIERTKTTISDAEIDRTLDILRKQRVHYHARGEAGEH
GDGGADTAAKDGDRVTVDFVGKIDGEAFQGGTADDFAFVLGEGRMLPEFEKAALGLKVGESKEFDLAFPEDYHGKEVAGK
TAQFAITMKKIEWPHLPEIDAEFAKSLGIEDGDLTKMRNEIRDNLEREAKRRTQAVVKNQVMDALLKISELDVPKALIEQ
DQQRLVEMARQDLVQRGVPNAKDAPIPAEMFAEQAERRVKLGLVLAELVKANELQAKPEQIRAEVDEFAKSYEDPKEVVR
WYYSNQQRLAEMEAYVVESNVVDFVLSKAKVTDKEVSFEELASATAQA
>Mature_447_residues
ANVVENLGKLERRVTISLPKDAVQKEVDSRIRQLAKNVRMPGFRPGKVPLKMVTQQYAGQVEAEVLSDKVGKQFFDISRA
ENLRVAGQPSFAPKADAVEGDYAFDATFEVYPEVKLGDVATAEIERTKTTISDAEIDRTLDILRKQRVHYHARGEAGEHG
DGGADTAAKDGDRVTVDFVGKIDGEAFQGGTADDFAFVLGEGRMLPEFEKAALGLKVGESKEFDLAFPEDYHGKEVAGKT
AQFAITMKKIEWPHLPEIDAEFAKSLGIEDGDLTKMRNEIRDNLEREAKRRTQAVVKNQVMDALLKISELDVPKALIEQD
QQRLVEMARQDLVQRGVPNAKDAPIPAEMFAEQAERRVKLGLVLAELVKANELQAKPEQIRAEVDEFAKSYEDPKEVVRW
YYSNQQRLAEMEAYVVESNVVDFVLSKAKVTDKEVSFEELASATAQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=443, Percent_Identity=40.8577878103837, Blast_Score=347, Evalue=9e-97,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BURP8 (B2JGL4)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857245.1
- ProteinModelPortal:   B2JGL4
- SMR:   B2JGL4
- GeneID:   6242508
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1010
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49915; Mature: 49784

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVENLGKLERRVTISLPKDAVQKEVDSRIRQLAKNVRMPGFRPGKVPLKMVTQQYAG
CCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
QVEAEVLSDKVGKQFFDISRAENLRVAGQPSFAPKADAVEGDYAFDATFEVYPEVKLGDV
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEECCEEEECCCCCCCCC
ATAEIERTKTTISDAEIDRTLDILRKQRVHYHARGEAGEHGDGGADTAAKDGDRVTVDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
GKIDGEAFQGGTADDFAFVLGEGRMLPEFEKAALGLKVGESKEFDLAFPEDYHGKEVAGK
ECCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCC
TAQFAITMKKIEWPHLPEIDAEFAKSLGIEDGDLTKMRNEIRDNLEREAKRRTQAVVKNQ
CCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDALLKISELDVPKALIEQDQQRLVEMARQDLVQRGVPNAKDAPIPAEMFAEQAERRVK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGLVLAELVKANELQAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAYVVESN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
VVDFVLSKAKVTDKEVSFEELASATAQA
HHHHHHHHHHCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ANVVENLGKLERRVTISLPKDAVQKEVDSRIRQLAKNVRMPGFRPGKVPLKMVTQQYAG
CHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHC
QVEAEVLSDKVGKQFFDISRAENLRVAGQPSFAPKADAVEGDYAFDATFEVYPEVKLGDV
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCEECCEEEECCCCCCCCC
ATAEIERTKTTISDAEIDRTLDILRKQRVHYHARGEAGEHGDGGADTAAKDGDRVTVDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
GKIDGEAFQGGTADDFAFVLGEGRMLPEFEKAALGLKVGESKEFDLAFPEDYHGKEVAGK
ECCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCC
TAQFAITMKKIEWPHLPEIDAEFAKSLGIEDGDLTKMRNEIRDNLEREAKRRTQAVVKNQ
CCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMDALLKISELDVPKALIEQDQQRLVEMARQDLVQRGVPNAKDAPIPAEMFAEQAERRVK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGLVLAELVKANELQAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAYVVESN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
VVDFVLSKAKVTDKEVSFEELASATAQA
HHHHHHHHHHCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA