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The map label for this gene is psdht [H]

Identifier: 186475759

GI number: 186475759

Start: 1113346

End: 1114368

Strand: Reverse

Name: psdht [H]

Synonym: Bphy_0994

Alternate gene names: 186475759

Gene position: 1114368-1113346 (Counterclockwise)

Preceding gene: 186475760

Following gene: 186475758

Centisome position: 32.03

GC content: 65.69

Gene sequence:

>1023_bases
ATGTCCACTGCCACGCCGCACCACACCGACCGCACGATCGACGGCGAGCCGATACCGACGCTCGACGATATCGCCTCGCA
GCACTTCGCGTTGACGCCGTGGGTGGTGCGAACGCCCGTATTCGACCGGATGGATTTTCCGACGCTCGAAGGCACGCTCG
TCAACTTCAAGTTCGAACTGCTACAGGCAGGCGGCAGCTTCAAGGCGCGCGGCGCGTTCACGAATCTGCTTGCGCTCGAC
GCGGCGCAACGCAGCGCGGGCGTCACCTGCGTGTCGGGCGGCAATCACGCCGTGGCCGTCGCGTACGCGGCAATGCGCAT
GGGCATTAGCGCGAAGGTCGTCGTGATGCATGCGGCGAATCCGGCGCGAATCGCGCTGTGCCGCCAGTACCGCGCGGAAG
TTGTGATGGCGGACAACGTGAACGACGCGTTCGAGATCGTACGGCGCATCGAAGCCGAGGAAGGCCGTCACTTCGTGCAT
CCGTTCAACGGCTACCGCACGGTGCTCGGCACTGCGACGCTCGGCTACGAATGGGCCACGCAGACACCCGACCTCGATGC
CGTGATCGTGCCCATCGGCGGCGGCGGACTCGCGGCGGGCGTTTCGACCGCGCTGCGCCTCGCGAATCCGCGTGTGCACG
TGTACGGCGTCGAGCCGGAAGGCGCGGATGTGATGAACCGCAGCTTCGCCGCGAATCACACCGTCAAGATGAGCCATATG
CATTCGATCGCCGATTCGCTGATGGCGCCGCACACGGAGCAATACAGCTACGAGCTGTGCCGCCGTCATATGGACCGTCT
CGTCACGGTCACGGACGACGCGCTGCGCGCCGCGATGCTCACGCTATTCAAGCAACTGAAGCTTGCCGTCGAACCGGCGT
GCGCAGCCGCCACGGCCGCCCTGCTGGGCCCGTTGCGCGAGCAACTGCAAGGCAAGCGGGTCGGCGTGCTGTTGTGCGGG
ACCAATACCGATCCCGTCACGTTCGCCGCGCATATCGAACAGGCGCGAGCGGCCGAAATCTGA

Upstream 100 bases:

>100_bases
GGTCGATGCGGCGCACATTGACCCAAGCAGTTGAGAAGGCAACAAGCACAACAACACGGCACAAGAACGCGGACCTCACC
CACTTGCGAGTACGCACGCC

Downstream 100 bases:

>100_bases
CTCGGTCATATGCCTGGTTGCTGCGGGCGCGGCGTTTGCAAACACCGACGGTTCTTCGTCACTTCTGCGACATATCCGCC
AACCGAACCGGCCATTTCCA

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSTATPHHTDRTIDGEPIPTLDDIASQHFALTPWVVRTPVFDRMDFPTLEGTLVNFKFELLQAGGSFKARGAFTNLLALD
AAQRSAGVTCVSGGNHAVAVAYAAMRMGISAKVVVMHAANPARIALCRQYRAEVVMADNVNDAFEIVRRIEAEEGRHFVH
PFNGYRTVLGTATLGYEWATQTPDLDAVIVPIGGGGLAAGVSTALRLANPRVHVYGVEPEGADVMNRSFAANHTVKMSHM
HSIADSLMAPHTEQYSYELCRRHMDRLVTVTDDALRAAMLTLFKQLKLAVEPACAAATAALLGPLREQLQGKRVGVLLCG
TNTDPVTFAAHIEQARAAEI

Sequences:

>Translated_340_residues
MSTATPHHTDRTIDGEPIPTLDDIASQHFALTPWVVRTPVFDRMDFPTLEGTLVNFKFELLQAGGSFKARGAFTNLLALD
AAQRSAGVTCVSGGNHAVAVAYAAMRMGISAKVVVMHAANPARIALCRQYRAEVVMADNVNDAFEIVRRIEAEEGRHFVH
PFNGYRTVLGTATLGYEWATQTPDLDAVIVPIGGGGLAAGVSTALRLANPRVHVYGVEPEGADVMNRSFAANHTVKMSHM
HSIADSLMAPHTEQYSYELCRRHMDRLVTVTDDALRAAMLTLFKQLKLAVEPACAAATAALLGPLREQLQGKRVGVLLCG
TNTDPVTFAAHIEQARAAEI
>Mature_339_residues
STATPHHTDRTIDGEPIPTLDDIASQHFALTPWVVRTPVFDRMDFPTLEGTLVNFKFELLQAGGSFKARGAFTNLLALDA
AQRSAGVTCVSGGNHAVAVAYAAMRMGISAKVVVMHAANPARIALCRQYRAEVVMADNVNDAFEIVRRIEAEEGRHFVHP
FNGYRTVLGTATLGYEWATQTPDLDAVIVPIGGGGLAAGVSTALRLANPRVHVYGVEPEGADVMNRSFAANHTVKMSHMH
SIADSLMAPHTEQYSYELCRRHMDRLVTVTDDALRAAMLTLFKQLKLAVEPACAAATAALLGPLREQLQGKRVGVLLCGT
NTDPVTFAAHIEQARAAEI

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=321, Percent_Identity=29.595015576324, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI19923959, Length=319, Percent_Identity=28.8401253918495, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=35.7933579335793, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790207, Length=301, Percent_Identity=29.5681063122924, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71991565, Length=320, Percent_Identity=31.875, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17508781, Length=314, Percent_Identity=28.0254777070064, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17537387, Length=316, Percent_Identity=30.0632911392405, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322631, Length=317, Percent_Identity=32.4921135646688, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=28.5211267605634, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320930, Length=280, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322024, Length=161, Percent_Identity=29.1925465838509, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355833, Length=320, Percent_Identity=34.375, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24645328, Length=223, Percent_Identity=33.6322869955157, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36681; Mature: 36550

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATPHHTDRTIDGEPIPTLDDIASQHFALTPWVVRTPVFDRMDFPTLEGTLVNFKFEL
CCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCEEEECCCCCCCCCCCCCCEEEEEEEEE
LQAGGSFKARGAFTNLLALDAAQRSAGVTCVSGGNHAVAVAYAAMRMGISAKVVVMHAAN
ECCCCCCEECHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHCCCCEEEEEEECCC
PARIALCRQYRAEVVMADNVNDAFEIVRRIEAEEGRHFVHPFNGYRTVLGTATLGYEWAT
CHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEHHCCCEEECC
QTPDLDAVIVPIGGGGLAAGVSTALRLANPRVHVYGVEPEGADVMNRSFAANHTVKMSHM
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCEEEHHHH
HSIADSLMAPHTEQYSYELCRRHMDRLVTVTDDALRAAMLTLFKQLKLAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LLGPLREQLQGKRVGVLLCGTNTDPVTFAAHIEQARAAEI
HHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure 
STATPHHTDRTIDGEPIPTLDDIASQHFALTPWVVRTPVFDRMDFPTLEGTLVNFKFEL
CCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCEEEECCCCCCCCCCCCCCEEEEEEEEE
LQAGGSFKARGAFTNLLALDAAQRSAGVTCVSGGNHAVAVAYAAMRMGISAKVVVMHAAN
ECCCCCCEECHHHHHHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHCCCCEEEEEEECCC
PARIALCRQYRAEVVMADNVNDAFEIVRRIEAEEGRHFVHPFNGYRTVLGTATLGYEWAT
CHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEHHCCCEEECC
QTPDLDAVIVPIGGGGLAAGVSTALRLANPRVHVYGVEPEGADVMNRSFAANHTVKMSHM
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCEEEHHHH
HSIADSLMAPHTEQYSYELCRRHMDRLVTVTDDALRAAMLTLFKQLKLAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LLGPLREQLQGKRVGVLLCGTNTDPVTFAAHIEQARAAEI
HHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]