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The map label for this gene is dmlR [H]

Identifier: 186475748

GI number: 186475748

Start: 1100016

End: 1100990

Strand: Reverse

Name: dmlR [H]

Synonym: Bphy_0983

Alternate gene names: 186475748

Gene position: 1100990-1100016 (Counterclockwise)

Preceding gene: 186475751

Following gene: 186475743

Centisome position: 31.65

GC content: 69.13

Gene sequence:

>975_bases
ATGACCACGCTTTCCCACATGGCGCCCGGGGCGTCCGCCGAGCGCGACCGGCTCGATCTGCTCGACGTCGCGCTGTTCGT
GCGCGCCGCCTTGCTCGCGAACGTGTCGGCGGCGGGACGCGATTTCGGCTTGTCGCCCGCCGTCGCCAGCGCTCGGATCG
CGAACCTGGAAAAACTGCTGGGGGCGCGTCTGCTGCATCGGACGACGCGTCGCGTCAGCCTCACGCAGGAAGGTGAGGTG
TTCATGGCGCGCGCCGAGACGCTGCTCGACGCGGCAGCCGCCGCGCGTGCTTCCGTCGGGCGCGGACAGGCCGAGCCGCA
GGGTCGGCTGCGCGTGTCGATGCCGTCGTCGTTCGGGCGGCAGCATGTGTCGCCCGTGATCGCAGCGTTCTTGCGGCGCT
ATCCCGGCGTGAGCGTCGACTTGCGGCTCACCGACACCATCGTCGATCTCGTCGATGCCGGCGTCGATGTGGGGATCCGG
CTGGGCGCGCTGAAGGATTCGACGCTGGTCGCGCGGCGCCTCGCGGCAAACCGCCGTGTGATCTGCTGCGCGCCGTCGTA
TCTCGCCGAACACGGCACGCCGCATCATCCGTCCGATCTCGCGCAGCACGAATGCGTGATCCTCGCCGATCAGCGCGACT
GGTCGTTCGTCACGCCCGCGGGGCCGCTGAGCGTACGCGTCGGCGGACGCCTCGCGACCGACAACGGCGAAGTCATTCGC
GATGCGCTGCTGGCGGGATTCGGTATCGCGCTGAAATCGACGTGGGACGTGGCGCCCTATCTGCGCAGCGGCGAGCTGGT
GACCGTGCTCGAGTCCTATCCGCTCGCCGAAACGGTCGCGATCTGGGCAGTGTATCCGTCGCGCGCCTTCGTGCCACCGA
AGACGGTCGCGTTCATCGACTTCCTCGCCGCGCACTTCGGCGATCCGCCGTATTGGGACAGCGGGGACAGCGATAGCAAC
CGGAACGCGCGCTGA

Upstream 100 bases:

>100_bases
CAAAGTGAAGCCGCTCGCGTTGACTCCACTGTAGAAAACTCTTTACGCTGCGAGAACCAACATAATCACTGAAACATTTT
CTGAAATATCCAAATAATCG

Downstream 100 bases:

>100_bases
ATGCCCGCGCGCCGCGCTCAACGGCGCTTGTCGTTCGTCCCCGCGGAATTTTCGTTGGCATGCGCATCGTTCTGCGTGCG
CTCCTGATAGTCGCCGCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTTLSHMAPGASAERDRLDLLDVALFVRAALLANVSAAGRDFGLSPAVASARIANLEKLLGARLLHRTTRRVSLTQEGEV
FMARAETLLDAAAAARASVGRGQAEPQGRLRVSMPSSFGRQHVSPVIAAFLRRYPGVSVDLRLTDTIVDLVDAGVDVGIR
LGALKDSTLVARRLAANRRVICCAPSYLAEHGTPHHPSDLAQHECVILADQRDWSFVTPAGPLSVRVGGRLATDNGEVIR
DALLAGFGIALKSTWDVAPYLRSGELVTVLESYPLAETVAIWAVYPSRAFVPPKTVAFIDFLAAHFGDPPYWDSGDSDSN
RNAR

Sequences:

>Translated_324_residues
MTTLSHMAPGASAERDRLDLLDVALFVRAALLANVSAAGRDFGLSPAVASARIANLEKLLGARLLHRTTRRVSLTQEGEV
FMARAETLLDAAAAARASVGRGQAEPQGRLRVSMPSSFGRQHVSPVIAAFLRRYPGVSVDLRLTDTIVDLVDAGVDVGIR
LGALKDSTLVARRLAANRRVICCAPSYLAEHGTPHHPSDLAQHECVILADQRDWSFVTPAGPLSVRVGGRLATDNGEVIR
DALLAGFGIALKSTWDVAPYLRSGELVTVLESYPLAETVAIWAVYPSRAFVPPKTVAFIDFLAAHFGDPPYWDSGDSDSN
RNAR
>Mature_323_residues
TTLSHMAPGASAERDRLDLLDVALFVRAALLANVSAAGRDFGLSPAVASARIANLEKLLGARLLHRTTRRVSLTQEGEVF
MARAETLLDAAAAARASVGRGQAEPQGRLRVSMPSSFGRQHVSPVIAAFLRRYPGVSVDLRLTDTIVDLVDAGVDVGIRL
GALKDSTLVARRLAANRRVICCAPSYLAEHGTPHHPSDLAQHECVILADQRDWSFVTPAGPLSVRVGGRLATDNGEVIRD
ALLAGFGIALKSTWDVAPYLRSGELVTVLESYPLAETVAIWAVYPSRAFVPPKTVAFIDFLAAHFGDPPYWDSGDSDSNR
NAR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=37.7551020408163, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=33.6805555555556, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=34.1296928327645, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=29.8969072164948, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=30.5555555555556, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=32.0557491289199, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=25.3424657534247, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789173, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=307, Percent_Identity=26.7100977198697, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786448, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34767; Mature: 34636

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSHMAPGASAERDRLDLLDVALFVRAALLANVSAAGRDFGLSPAVASARIANLEKLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GARLLHRTTRRVSLTQEGEVFMARAETLLDAAAAARASVGRGQAEPQGRLRVSMPSSFGR
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHH
QHVSPVIAAFLRRYPGVSVDLRLTDTIVDLVDAGVDVGIRLGALKDSTLVARRLAANRRV
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCEE
ICCAPSYLAEHGTPHHPSDLAQHECVILADQRDWSFVTPAGPLSVRVGGRLATDNGEVIR
EEECHHHHHHCCCCCCCHHHCCCCEEEEECCCCCCEECCCCCEEEEECCEEECCCHHHHH
DALLAGFGIALKSTWDVAPYLRSGELVTVLESYPLAETVAIWAVYPSRAFVPPKTVAFID
HHHHHHHHHHCCCCCCCCCHHCCCCEEEEECCCCCHHHEEEEEECCCCCCCCCHHHHHHH
FLAAHFGDPPYWDSGDSDSNRNAR
HHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTLSHMAPGASAERDRLDLLDVALFVRAALLANVSAAGRDFGLSPAVASARIANLEKLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GARLLHRTTRRVSLTQEGEVFMARAETLLDAAAAARASVGRGQAEPQGRLRVSMPSSFGR
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHH
QHVSPVIAAFLRRYPGVSVDLRLTDTIVDLVDAGVDVGIRLGALKDSTLVARRLAANRRV
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCEE
ICCAPSYLAEHGTPHHPSDLAQHECVILADQRDWSFVTPAGPLSVRVGGRLATDNGEVIR
EEECHHHHHHCCCCCCCHHHCCCCEEEEECCCCCCEECCCCCEEEEECCEEECCCHHHHH
DALLAGFGIALKSTWDVAPYLRSGELVTVLESYPLAETVAIWAVYPSRAFVPPKTVAFID
HHHHHHHHHHCCCCCCCCCHHCCCCEEEEECCCCCHHHEEEEEECCCCCCCCCHHHHHHH
FLAAHFGDPPYWDSGDSDSNRNAR
HHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]