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The map label for this gene is rho [H]

Identifier: 186475714

GI number: 186475714

Start: 1059870

End: 1061132

Strand: Reverse

Name: rho [H]

Synonym: Bphy_0949

Alternate gene names: 186475714

Gene position: 1061132-1059870 (Counterclockwise)

Preceding gene: 186475715

Following gene: 186475713

Centisome position: 30.5

GC content: 59.78

Gene sequence:

>1263_bases
ATGCATTTATCCGAGCTTAAGACTCTGCACGTGTCCCAATTGATCGAGATGGCCAATGGTCTCGAGATCGAAAGTGCGAA
CCGCCTGCGCAAGCAGGAGCTGATGTTCGCCATTCTAAAAAAACGCGCCAAAACAGGCGAAACGATCTTCGGTGACGGCA
CGCTCGAAGTGCTGCCGGACGGCTTCGGTTTCCTGCGCTCGCCGGAAACCTCCTATCTCGCCAGCACGGACGACATCTAC
ATCAGTCCGTCGCAGATCCGCCGCTTCAACCTGCATACGGGCGACACCATCGAAGGCGAAGTCCGCACGCCGAAGGACGG
CGAGCGCTACTTTGCGCTGGTGAAGGTCGACAAGGTCAACGGCCAGCCGCCCGAGGCCTCGAAGCACAAGATCATGTTCG
AGAACCTGACGCCGCTGCACCCGAACAAGGTGCTGCTGCTCGAACGTGAAATGCGTGGCGAAGAAAACGTCACCGGCCGC
ATCATCGACATGATCGCGCCCATCGGCAAAGGCCAGCGCGGCCTGCTCGTCGCGTCGCCGAAGTCCGGCAAGACGGTGAT
GCTTCAGCACATCGCGCATGCCATCAAGCAGAACCATCCGGACGTGATCCTGTTCGTGCTGCTGATCGACGAACGTCCGG
AAGAAGTGACGGAAATGCAACGCTCGGTGGCGGGCGAAGTGATCGCGTCGACCTTCGACGAACCGGCCGCGCGTCACGTG
CAGGTCGCCGAAATGGTGATCGAAAAGGCGAAACGCCTCGTCGAAATGAAGAACGACGTCGTGATTCTGCTCGACTCGAT
CACGCGTCTCGCGCGTGCGTACAACACGGTTGTGCCGGCATCGGGCAAGGTGCTGACGGGCGGCGTCGACGCGAACGCGC
TGCAGCGTCCGAAGCGCTTCTTCGGCGCGGCGCGCAACATCGAGGAAGGTGGTTCGCTGACCATCATCGGCACGGCGCTG
ATCGAAACGGGCAGCCGCATGGACGACGTGATCTACGAAGAGTTCAAGGGCACGGGCAACATGGAAGTGCACCTCGAGCG
CCGTCTCGCGGAAAAGCGCGTCTATCCGTCGATCAATCTGAACAAGTCCGGCACGCGTCGCGAAGAACTGCTGATCAAGC
CCGAGATCCTGCAAAAGATCTGGGTGCTGCGCAAGTTCATTCACGACATGGACGAAGTCGAAGCCATGGAATTCCTGCTC
GACAAGATCCGCCAGACGAAGAACAACGCGGAGTTCTTCGACATGATGCGTCGCGGCGGCTAA

Upstream 100 bases:

>100_bases
TCCATAAAACGTCTAAAAGACGAGTATGTCCTTGAGCGGTTCCGCTCACTTCTCCTCCCAGATTTCAATTCAGGTCGCAC
CTTCTCTGCGGGTTCTCCGT

Downstream 100 bases:

>100_bases
GCGCCCCCTACGTTTCATCCGCAAAACGCCTGCCTGCGCAGGCGTTTTTCTTTTACGCGCTGCGTGCCGGCGCGGACAAA
GGCGCGAAGGCATGAGCGCC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MHLSELKTLHVSQLIEMANGLEIESANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKVLLLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPAARHV
QVAEMVIEKAKRLVEMKNDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREELLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKNNAEFFDMMRRGG

Sequences:

>Translated_420_residues
MHLSELKTLHVSQLIEMANGLEIESANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKVLLLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPAARHV
QVAEMVIEKAKRLVEMKNDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREELLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKNNAEFFDMMRRGG
>Mature_420_residues
MHLSELKTLHVSQLIEMANGLEIESANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPETSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKVLLLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPAARHV
QVAEMVIEKAKRLVEMKNDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREELLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKNNAEFFDMMRRGG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=69.6172248803828, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47331; Mature: 47331

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSELKTLHVSQLIEMANGLEIESANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPD
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCC
GFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCCCEEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECC
GQPPEASKHKIMFENLTPLHPNKVLLLEREMRGEENVTGRIIDMIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCEEEECC
KSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPAARHV
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QVAEMVIEKAKRLVEMKNDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIGTALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINL
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCC
NKSGTRREELLIKPEILQKIWVLRKFIHDMDEVEAMEFLLDKIRQTKNNAEFFDMMRRGG
CCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHLSELKTLHVSQLIEMANGLEIESANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPD
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCC
GFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCCCEEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECC
GQPPEASKHKIMFENLTPLHPNKVLLLEREMRGEENVTGRIIDMIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCEEEECC
KSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPAARHV
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QVAEMVIEKAKRLVEMKNDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIGTALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINL
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCC
NKSGTRREELLIKPEILQKIWVLRKFIHDMDEVEAMEFLLDKIRQTKNNAEFFDMMRRGG
CCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]