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The map label for this gene is phoB [H]

Identifier: 186475652

GI number: 186475652

Start: 991355

End: 992056

Strand: Direct

Name: phoB [H]

Synonym: Bphy_0887

Alternate gene names: 186475652

Gene position: 991355-992056 (Clockwise)

Preceding gene: 186475651

Following gene: 186475653

Centisome position: 28.49

GC content: 62.82

Gene sequence:

>702_bases
ATGCCCAGCAGTATTCTCGTTATCGAAGATGAACCCGCCATTTCCGAGCTGATCTCGGTCAACTTGCAACACGCGGGCCA
TTGTCCGATTCGTGCGTACAACGCCGAACAGGCGCAGAACCTGATCAGCGATGTGCTTCCCGATCTCGTGCTGCTCGACT
GGATGCTGCCGGGCAAATCGGGCATTGCGTTCGCGCGCGACCTGCGCAACAACGAGCGTACGAAGCACATCCCGATCATC
ATGCTGACGGCGCGCGGCGATGAGCAGGACAAGGTGCTCGGGCTCGAGATCGGCGCCGACGACTACGTGACCAAGCCGTT
TTCGCCGAAGGAGCTGATGGCGCGTATCAAGGCGGTCCTGCGCCGCCGTGCGCCGCAGCTGACGGAAGACGTCGTTGCGA
TCAATGGACTGAAGCTCGACCCGGCCACGCACCGCGTCGCCGCGCACGCGCAAGGCAGCGAGATCAAGCTCGATCTCGGC
CCGACGGAATTCCGCTTGCTGCACTTCTTCATGACGCACCCCGAGCGCGTGCACAGCCGCACGCAATTGCTCGACCAGGT
GTGGGGCGATCACGTGTTCGTCGAAGAGCGTACTGTGGACGTGCACATCAAGCGTCTGCGGGCCGCGCTGAAGCCGGCGG
GCTGCGATGCGATGATCGAAACGGTGCGCGGCAGCGGCTATCGCCTGGCCAAAAGCGCATAA

Upstream 100 bases:

>100_bases
GGGTCGCGACGCGCTCGAGCGCGAAGCGCTCAGCTGATCCGTCTGACGTTCAGCGATTCCGCACAGCAAAACCGTATATA
CCGTTTATCAAGAGGCGCCG

Downstream 100 bases:

>100_bases
GGTAAACTGATCGAAAAACACGAAGCGCGCTGCGGGCACTCGGACGCAGCGCGCTTTGTTGTCTATGAAGTCTTCAGCTT
GTCTTCAAGGTCGCACATGA

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAQGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA

Sequences:

>Translated_233_residues
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAQGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
>Mature_232_residues
PSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPIIM
LTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLKLDPATHRVAAHAQGSEIKLDLGP
TEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=57.8260869565217, Blast_Score=283, Evalue=9e-78,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=41.5584415584416, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=38.5281385281385, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=38.1578947368421, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI2367329, Length=240, Percent_Identity=37.0833333333333, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=32.7354260089686, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=34.5291479820628, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=34.7639484978541, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1789402, Length=228, Percent_Identity=34.6491228070175, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=31.1475409836066, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25996; Mature: 25865

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLKLDPATHRVAAHAQGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHCCCEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
PSSILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCEEEEECCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLKLDPATHRVAAHAQGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHCCCEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKSA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]