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The map label for this gene is gloB [H]

Identifier: 186475635

GI number: 186475635

Start: 969864

End: 970667

Strand: Direct

Name: gloB [H]

Synonym: Bphy_0870

Alternate gene names: 186475635

Gene position: 969864-970667 (Clockwise)

Preceding gene: 186475628

Following gene: 186475636

Centisome position: 27.88

GC content: 64.68

Gene sequence:

>804_bases
ATGAATTCGCTCGAGTACGTGCCCGTCCCGGCGTTTGATGACAATTACATCTGGGTCGTGTCGGATGGACATCATGCCGT
CGTCGTCGATCCGGGCGACGCCGCCCCGGTGAAGGCCTATCTGGCGAAACGGGGATGGCGGCTGAGCGCTATTTTACTCA
CCCACCATCATGCCGACCACGTCGGCGGAGTAGCCGATCTGCTGAACGGCCAGGATGCGAGCCAGGCTTTGCCCGTCTAC
GGGCCGGCGGGAGAAGCGATTCCGTGGCTGACGGAACGCCTCGTGCAGGGCGACACCGTGCATATCGCCGCGCCTTCTCT
CACTTTCACGATTCTCGACGTGCCCGGTCATACGCGCGGGCACATAGCGTATTTCCAGGCGGCCGACTCGCGCGGCACGC
CGCACGTGTTTTGCGGCGACACACTGTTCGCTTGCGGCTGCGGCCGGCTGTTCGAAGGGACGCCAGCGCAGATGCTCGAG
TCGCTGGATGCGCTCGCCGCACTGCCCGGCGAAACGGAGGTGCACTGCGCGCACGAATACACGCTATCGAATATCCGCTT
CGCGCTCGCCTGCGAGCCTGACAACCCCGTCCTGCGAGCCTGGCGCGACGAAGCGGCGGCACTGCGCGCCCGTCACATGC
CGACGCTGCCCACGACGATCGCACACGAGCGTGCAGTGAACCCGTTCTTGCGGGCGGGCGAGGCCTCCGTGCAATCGACG
CTCGCCGAGCAGCTACATGAATCAGTGCCGGACCGGTTGGCCGCTTTCACATTGATGCGCGAGTGGAAGAACAGGTTCCG
CTAA

Upstream 100 bases:

>100_bases
CGCCTGCTCCCACTCCAGCACATAGCGTCCGGGCGGGGAGGTGGTCCAGGCGGGCCAGTCTATAATCGATCGGTCAGACA
TGTTAAAGAATGCGTCGCCT

Downstream 100 bases:

>100_bases
CCGCTCCATTCATGGGGGCATGCTCAGCCAAATCGTAGAAATGATCGCCAAGCCTCAGATTTGGCAGACTTTTTCACATC
TTTCTGATTGACGGGAACGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNSLEYVPVPAFDDNYIWVVSDGHHAVVVDPGDAAPVKAYLAKRGWRLSAILLTHHHADHVGGVADLLNGQDASQALPVY
GPAGEAIPWLTERLVQGDTVHIAAPSLTFTILDVPGHTRGHIAYFQAADSRGTPHVFCGDTLFACGCGRLFEGTPAQMLE
SLDALAALPGETEVHCAHEYTLSNIRFALACEPDNPVLRAWRDEAAALRARHMPTLPTTIAHERAVNPFLRAGEASVQST
LAEQLHESVPDRLAAFTLMREWKNRFR

Sequences:

>Translated_267_residues
MNSLEYVPVPAFDDNYIWVVSDGHHAVVVDPGDAAPVKAYLAKRGWRLSAILLTHHHADHVGGVADLLNGQDASQALPVY
GPAGEAIPWLTERLVQGDTVHIAAPSLTFTILDVPGHTRGHIAYFQAADSRGTPHVFCGDTLFACGCGRLFEGTPAQMLE
SLDALAALPGETEVHCAHEYTLSNIRFALACEPDNPVLRAWRDEAAALRARHMPTLPTTIAHERAVNPFLRAGEASVQST
LAEQLHESVPDRLAAFTLMREWKNRFR
>Mature_267_residues
MNSLEYVPVPAFDDNYIWVVSDGHHAVVVDPGDAAPVKAYLAKRGWRLSAILLTHHHADHVGGVADLLNGQDASQALPVY
GPAGEAIPWLTERLVQGDTVHIAAPSLTFTILDVPGHTRGHIAYFQAADSRGTPHVFCGDTLFACGCGRLFEGTPAQMLE
SLDALAALPGETEVHCAHEYTLSNIRFALACEPDNPVLRAWRDEAAALRARHMPTLPTTIAHERAVNPFLRAGEASVQST
LAEQLHESVPDRLAAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=266, Percent_Identity=36.8421052631579, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI94538322, Length=266, Percent_Identity=36.8421052631579, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI116642887, Length=245, Percent_Identity=37.1428571428571, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI21703352, Length=245, Percent_Identity=37.1428571428571, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI14150041, Length=234, Percent_Identity=38.8888888888889, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI46361987, Length=195, Percent_Identity=39.4871794871795, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=42.8571428571429, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787158, Length=166, Percent_Identity=34.9397590361446, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=263, Percent_Identity=35.7414448669202, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320478, Length=273, Percent_Identity=30.03663003663, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324614, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356335, Length=271, Percent_Identity=39.4833948339483, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24667711, Length=271, Percent_Identity=39.4833948339483, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24667703, Length=243, Percent_Identity=42.3868312757202, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29119; Mature: 29119

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLEYVPVPAFDDNYIWVVSDGHHAVVVDPGDAAPVKAYLAKRGWRLSAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCHH
VGGVADLLNGQDASQALPVYGPAGEAIPWLTERLVQGDTVHIAAPSLTFTILDVPGHTRG
HCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCC
HIAYFQAADSRGTPHVFCGDTLFACGCGRLFEGTPAQMLESLDALAALPGETEVHCAHEY
CEEEEEECCCCCCCEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
TLSNIRFALACEPDNPVLRAWRDEAAALRARHMPTLPTTIAHERAVNPFLRAGEASVQST
ECCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCHHHHHHH
LAEQLHESVPDRLAAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSLEYVPVPAFDDNYIWVVSDGHHAVVVDPGDAAPVKAYLAKRGWRLSAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCHH
VGGVADLLNGQDASQALPVYGPAGEAIPWLTERLVQGDTVHIAAPSLTFTILDVPGHTRG
HCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCC
HIAYFQAADSRGTPHVFCGDTLFACGCGRLFEGTPAQMLESLDALAALPGETEVHCAHEY
CEEEEEECCCCCCCEEEECCHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
TLSNIRFALACEPDNPVLRAWRDEAAALRARHMPTLPTTIAHERAVNPFLRAGEASVQST
ECCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHCCHHHHHHH
LAEQLHESVPDRLAAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA