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The map label for this gene is mltD [H]

Identifier: 186475636

GI number: 186475636

Start: 970823

End: 972508

Strand: Direct

Name: mltD [H]

Synonym: Bphy_0871

Alternate gene names: 186475636

Gene position: 970823-972508 (Clockwise)

Preceding gene: 186475635

Following gene: 186475637

Centisome position: 27.9

GC content: 63.64

Gene sequence:

>1686_bases
ATGAGATTCATCTTTTGCGCGTTGTTGGTCCTGACGCTCGCCGCATGCGCGAGCCAGGGGCCTACGACGAATAGCCTTAC
CACTGCTTCCAATCCCGCTAGCCAGCAAGCCGTAGCCGACGCCCTCCGCAAGACCGCCACCGCCAAAGAAACCATCAACG
TCGACCAGGGTTCCGTCGACCAGTTGACGAGCGCAGACAGCGATCTTTGGGGCCGTATCCGTCGTGGTTTCCAGATGCCG
GACCTGCAAAGCGACCTCGTCGACATGCAGGCGAACTGGTACGCGCAGCGCCCGGATTACGTGCAGCGCATGACCGAACG
GTCGCAGAAGTACCTGTACCACATCGTCGAAGAACTCGAAGCGCGCCACATGCCGACGGAGCTCGCGCTGCTGCCGTTCA
TCGAATCCGCGTACAACCCGCAGGCGCTGTCTGTCGCGAAGGCGGCAGGCATGTGGCAGTTCGTGCCGGGCACGGGCCGA
ACGTACAACCTCAAGCAGAACATGTGGCAGGACGAACGCCGCGACGTGCTCGCGTCCACCAGTGCCGCGCTCGACTATCT
GTCGCGCCTGCACGATATGTTCGGCGACTGGTATCTGGCGCTCGCCGCGTACAACTGGGGCGAGGGCAACGTGCAGCGCG
CGATCGCGCGCAACGAAGCCGCCGGTCTGCCGACGGACTACCAGAGCCTGCGCATGCCGATGGAAACGCGCAACTACGTG
CCGAAGTTGCAGGCGGTGAAGAACATCGTGGCGAACCCGCAGGTCTACGGGCTCACGCTGCCGTCCATTCCGAACCACCC
GTACTTCGTCACCGTCACGACGTCGCACGATATCGACGTCGACACGGCGGCGAAGCTTGCGAACATGACGCCGGACGAAT
TCCGTTCGCTGAATCCGTCGTTCAAGAAACCGGTCATTCTCGGCGCGACGCAGCCGCAGATTCTGCTGCCGTTCGACAAT
GCCAGCGCCTTCGAGCGCAACCTGAAGTCTTACTCGGGCTCGCTGTCGTCATGGACGACCTATACGGTCACCGAGCGCAG
TCGTCCCGCCGCGATCGCCGAAAAGATCGGTGTCGACGCCGATACGCTGATGGCCGTGAACAAGATTCCGGCGGGCATGC
GCCTGAAGCCGGGTTCGACGATCGTCGTGCCGCGCGCCGATGACGACGACGAAGACATCAGCGCCGACGTTGCCGAAAGC
GCCACGCTAGCGATGGAGCCAGACGTGCCCGACACGCGCAAGATGCTGATTCGCGTGCGTCGCAAGCAGTCGATGGCGGT
GCTGGCCGATCGCTACGATGTGTCGATCGGTCAGTTGAAGGCTTGGAACCGCACCAGGCGCGACGTGGTGATGCCGGGTC
AGGTCGTCGTGCTGCACGTGCCCGTCGGCAAGGCGATGCCGAGCGAGCCGGGCCCGCAGAAGCTGGCCACGGTGCCCGTC
GGCGGTGGCGTGGAGAAGGCCGGCGCCCAGATCGCGGATACGCATTCTGAATCGCGCTACGATAAGAAGCGAGGCCGCGG
CCACACTGGCATGGTCAAAGTGTCGGAACCGGTCGGCAAGACCGACAAGCCCGCTCCCGCAAAAGGCAAGGTGACGAAGG
TTTCGACGGAAGCGGCTGGCAAGGCGTCGAAGGCGGAGACGGGCAGCCACCACAAGGTCTCGGCCAGCGCGAAGAAGAGC
AAGTAA

Upstream 100 bases:

>100_bases
CAGATTTGGCAGACTTTTTCACATCTTTCTGATTGACGGGAACGGCGCACTTTCGTACTATCGGCTGCAAATTCCAGCCC
CTTTGGAAGCCGAGACGTTC

Downstream 100 bases:

>100_bases
AGAACAAGAGTACTTTCGAGGATGACCAAACACGCGTGCGGGGGCATCGCGTGAAGTGCGCTCCGATCTCGCCTGATTTC
CAGAACGCCGTCCCGTCAAA

Product: lytic transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRFIFCALLVLTLAACASQGPTTNSLTTASNPASQQAVADALRKTATAKETINVDQGSVDQLTSADSDLWGRIRRGFQMP
DLQSDLVDMQANWYAQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFVPGTGR
TYNLKQNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNEAAGLPTDYQSLRMPMETRNYV
PKLQAVKNIVANPQVYGLTLPSIPNHPYFVTVTTSHDIDVDTAAKLANMTPDEFRSLNPSFKKPVILGATQPQILLPFDN
ASAFERNLKSYSGSLSSWTTYTVTERSRPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRADDDDEDISADVAES
ATLAMEPDVPDTRKMLIRVRRKQSMAVLADRYDVSIGQLKAWNRTRRDVVMPGQVVVLHVPVGKAMPSEPGPQKLATVPV
GGGVEKAGAQIADTHSESRYDKKRGRGHTGMVKVSEPVGKTDKPAPAKGKVTKVSTEAAGKASKAETGSHHKVSASAKKS
K

Sequences:

>Translated_561_residues
MRFIFCALLVLTLAACASQGPTTNSLTTASNPASQQAVADALRKTATAKETINVDQGSVDQLTSADSDLWGRIRRGFQMP
DLQSDLVDMQANWYAQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFVPGTGR
TYNLKQNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNEAAGLPTDYQSLRMPMETRNYV
PKLQAVKNIVANPQVYGLTLPSIPNHPYFVTVTTSHDIDVDTAAKLANMTPDEFRSLNPSFKKPVILGATQPQILLPFDN
ASAFERNLKSYSGSLSSWTTYTVTERSRPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRADDDDEDISADVAES
ATLAMEPDVPDTRKMLIRVRRKQSMAVLADRYDVSIGQLKAWNRTRRDVVMPGQVVVLHVPVGKAMPSEPGPQKLATVPV
GGGVEKAGAQIADTHSESRYDKKRGRGHTGMVKVSEPVGKTDKPAPAKGKVTKVSTEAAGKASKAETGSHHKVSASAKKS
K
>Mature_561_residues
MRFIFCALLVLTLAACASQGPTTNSLTTASNPASQQAVADALRKTATAKETINVDQGSVDQLTSADSDLWGRIRRGFQMP
DLQSDLVDMQANWYAQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFVPGTGR
TYNLKQNMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNEAAGLPTDYQSLRMPMETRNYV
PKLQAVKNIVANPQVYGLTLPSIPNHPYFVTVTTSHDIDVDTAAKLANMTPDEFRSLNPSFKKPVILGATQPQILLPFDN
ASAFERNLKSYSGSLSSWTTYTVTERSRPAAIAEKIGVDADTLMAVNKIPAGMRLKPGSTIVVPRADDDDEDISADVAES
ATLAMEPDVPDTRKMLIRVRRKQSMAVLADRYDVSIGQLKAWNRTRRDVVMPGQVVVLHVPVGKAMPSEPGPQKLATVPV
GGGVEKAGAQIADTHSESRYDKKRGRGHTGMVKVSEPVGKTDKPAPAKGKVTKVSTEAAGKASKAETGSHHKVSASAKKS
K

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=410, Percent_Identity=34.390243902439, Blast_Score=226, Evalue=4e-60,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 61401; Mature: 61401

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIFCALLVLTLAACASQGPTTNSLTTASNPASQQAVADALRKTATAKETINVDQGSVD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHH
QLTSADSDLWGRIRRGFQMPDLQSDLVDMQANWYAQRPDYVQRMTERSQKYLYHIVEELE
HHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARHMPTELALLPFIESAYNPQALSVAKAAGMWQFVPGTGRTYNLKQNMWQDERRDVLAST
HHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHHHH
SAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNEAAGLPTDYQSLRMPMETRNYV
HHHHHHHHHHHHHHHHHEEHHEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHCC
PKLQAVKNIVANPQVYGLTLPSIPNHPYFVTVTTSHDIDVDTAAKLANMTPDEFRSLNPS
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCC
FKKPVILGATQPQILLPFDNASAFERNLKSYSGSLSSWTTYTVTERSRPAAIAEKIGVDA
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCH
DTLMAVNKIPAGMRLKPGSTIVVPRADDDDEDISADVAESATLAMEPDVPDTRKMLIRVR
HHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHH
RKQSMAVLADRYDVSIGQLKAWNRTRRDVVMPGQVVVLHVPVGKAMPSEPGPQKLATVPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCEEEECC
GGGVEKAGAQIADTHSESRYDKKRGRGHTGMVKVSEPVGKTDKPAPAKGKVTKVSTEAAG
CCCHHHHCCHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCC
KASKAETGSHHKVSASAKKSK
CCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MRFIFCALLVLTLAACASQGPTTNSLTTASNPASQQAVADALRKTATAKETINVDQGSVD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCHH
QLTSADSDLWGRIRRGFQMPDLQSDLVDMQANWYAQRPDYVQRMTERSQKYLYHIVEELE
HHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARHMPTELALLPFIESAYNPQALSVAKAAGMWQFVPGTGRTYNLKQNMWQDERRDVLAST
HHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEECHHHHHHHHHHHHHHHHH
SAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNEAAGLPTDYQSLRMPMETRNYV
HHHHHHHHHHHHHHHHHEEHHEEECCCCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHCC
PKLQAVKNIVANPQVYGLTLPSIPNHPYFVTVTTSHDIDVDTAAKLANMTPDEFRSLNPS
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCC
FKKPVILGATQPQILLPFDNASAFERNLKSYSGSLSSWTTYTVTERSRPAAIAEKIGVDA
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCH
DTLMAVNKIPAGMRLKPGSTIVVPRADDDDEDISADVAESATLAMEPDVPDTRKMLIRVR
HHHHHHHCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHH
RKQSMAVLADRYDVSIGQLKAWNRTRRDVVMPGQVVVLHVPVGKAMPSEPGPQKLATVPV
HHHHHHHHHHHCCCCHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCEEEECC
GGGVEKAGAQIADTHSESRYDKKRGRGHTGMVKVSEPVGKTDKPAPAKGKVTKVSTEAAG
CCCHHHHCCHHHHCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEHHHCC
KASKAETGSHHKVSASAKKSK
CCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]