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The map label for this gene is rnhA [H]

Identifier: 186475633

GI number: 186475633

Start: 968586

End: 969032

Strand: Reverse

Name: rnhA [H]

Synonym: Bphy_0868

Alternate gene names: 186475633

Gene position: 969032-968586 (Counterclockwise)

Preceding gene: 186475634

Following gene: 186475632

Centisome position: 27.85

GC content: 61.74

Gene sequence:

>447_bases
ATGAGTTCTGATCTGATCGAAATCTTCACCGACGGCGCCTGCAAGGGCAATCCGGGGCCCGGCGGCTGGGGCGCGCTATT
GCGCTACGGCACGCAGGAAAAGGAATTGTTCGGCGGTGAAGCGAACACAACCAACAACAGAATGGAACTGATGGCCGTGA
TCGCTGCGCTCGAGGCGCTGAAGCGTCCATGCAAGGCCGTCGTGCACACCGATTCGCAATACGTGCAGAAAGGCATCAGC
GAATGGATCCACGGCTGGAAGAAAAAAGGCTGGGTAACGGCGGCGAGGGCGCCCGTGAAAAACGCCGATCTCTGGAAGCG
GCTCGACGCGCTCACGCAGCAGCATCAGCTCGAGTGGCGCTGGGTCAAGGGACACGCGGGGCATCCGGAGAACGAGCGCG
CGGATGCGCTAGCCAATCGCGGCGTGGCTTCGCTGGCAGATCTCTGA

Upstream 100 bases:

>100_bases
TGCGCGGGATGCGCCTCGTCGGTCCACTGAAAGTCAAGAAGCCCGTGCTGGCCGCCGGCCTCGCGCCCGCCGCCACACCC
AACACCCGCAACAAGACCCA

Downstream 100 bases:

>100_bases
CGCGCGCCCGCGGCTTGTTTCGAAGCATCCTATTTTTTTCACGCATCAACACAGGCAGGCAACACGATATGCGTCAAATC
ATCCTCGATACGGAAACGAC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSSDLIEIFTDGACKGNPGPGGWGALLRYGTQEKELFGGEANTTNNRMELMAVIAALEALKRPCKAVVHTDSQYVQKGIS
EWIHGWKKKGWVTAARAPVKNADLWKRLDALTQQHQLEWRWVKGHAGHPENERADALANRGVASLADL

Sequences:

>Translated_148_residues
MSSDLIEIFTDGACKGNPGPGGWGALLRYGTQEKELFGGEANTTNNRMELMAVIAALEALKRPCKAVVHTDSQYVQKGIS
EWIHGWKKKGWVTAARAPVKNADLWKRLDALTQQHQLEWRWVKGHAGHPENERADALANRGVASLADL
>Mature_147_residues
SSDLIEIFTDGACKGNPGPGGWGALLRYGTQEKELFGGEANTTNNRMELMAVIAALEALKRPCKAVVHTDSQYVQKGISE
WIHGWKKKGWVTAARAPVKNADLWKRLDALTQQHQLEWRWVKGHAGHPENERADALANRGVASLADL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=155, Percent_Identity=36.7741935483871, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=66.9172932330827, Blast_Score=197, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=139, Percent_Identity=39.568345323741, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=34.4594594594595, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=34.4594594594595, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16330; Mature: 16198

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDLIEIFTDGACKGNPGPGGWGALLRYGTQEKELFGGEANTTNNRMELMAVIAALEAL
CCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCKAVVHTDSQYVQKGISEWIHGWKKKGWVTAARAPVKNADLWKRLDALTQQHQLEWR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCEE
WVKGHAGHPENERADALANRGVASLADL
EECCCCCCCCCHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
SSDLIEIFTDGACKGNPGPGGWGALLRYGTQEKELFGGEANTTNNRMELMAVIAALEAL
CCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCKAVVHTDSQYVQKGISEWIHGWKKKGWVTAARAPVKNADLWKRLDALTQQHQLEWR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCEE
WVKGHAGHPENERADALANRGVASLADL
EECCCCCCCCCHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]