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The map label for this gene is otsB [H]

Identifier: 186475623

GI number: 186475623

Start: 956492

End: 957241

Strand: Direct

Name: otsB [H]

Synonym: Bphy_0858

Alternate gene names: 186475623

Gene position: 956492-957241 (Clockwise)

Preceding gene: 186475619

Following gene: 186475624

Centisome position: 27.49

GC content: 64.13

Gene sequence:

>750_bases
ATGCAAGCACTTCCCGCTTTTCTGTCTCCGACGGAGACGGCTTTTTTCTTCGATTTCGACGGCACGCTCGTCGATCTGGC
ACCGACACCCGATGGCGTACTCGTGCAGCCGGAGGCGGTCGCGTTGCTCGCCGAATTGCGGCGTCTGACCAACGGCGCGG
TGGCGATCCTCTCGGGTCGGGGCATCGACAGCATCGACCAGTTTCTCGGCATGCCCGATCTGCCCATCGCGGGTTTGCAC
GGCGCCGAACGGCGCGACGCGAACGGCGACACGCAGCGCATCGGCTTCAACGACGAGCGGCTGCTGCGCATGGAGCAGGT
GCTCGCGGACATCGTCAATGCGAATCCCGGCATGCTGCTCGAAATCAAGGGCGCGGCGCTTGCCTTGCACTATCGCAATG
CGCCCGATCGCGAGCCGGTTGCACGTGAAGCCACCACGCGCCTGGTCGCCGACTATCCGGAAGCATATGTGTTGCAGCCG
GGCAAGATGGTCTACGAAATCAAACCAAAGGATGTCGATAAAGGCCGCGCGCTGCGCGCCTTTCTCGACGAGCCGCCGTT
TACAGGCCGCACGCCCATTTTCGCCGGCGACGATCTCACCGACGAAAAAGGCTTCGCGGTCGTCAACGACCACGGCGGTC
TGTCGATCAAGGTCGGCAGCGGCGACACGATCGCACGCTCGCGCATCGGCTCGGTGAGTGCGCTGCTCGCGTGGCTGTCC
GAAATCGTCGCGGCGGCACGCAGCGCATGA

Upstream 100 bases:

>100_bases
TCGTGCCGCGTCGAAGCTGACTTTCTCGAAAAAACGACCGATGCGCGGTAAGATGCGCACACTTTTCCGGCCGGAAGCGG
CTGAGAGGCAACGTACACCG

Downstream 100 bases:

>100_bases
CCACGACCGTTCATCCGCGCCGTGCCCTTGTTCAGCACGGAAGTCACGCAACATGAGCCGATTGATCATCGTATCGAACC
GCGTCGCGCCAATTTCGGAA

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MQALPAFLSPTETAFFFDFDGTLVDLAPTPDGVLVQPEAVALLAELRRLTNGAVAILSGRGIDSIDQFLGMPDLPIAGLH
GAERRDANGDTQRIGFNDERLLRMEQVLADIVNANPGMLLEIKGAALALHYRNAPDREPVAREATTRLVADYPEAYVLQP
GKMVYEIKPKDVDKGRALRAFLDEPPFTGRTPIFAGDDLTDEKGFAVVNDHGGLSIKVGSGDTIARSRIGSVSALLAWLS
EIVAAARSA

Sequences:

>Translated_249_residues
MQALPAFLSPTETAFFFDFDGTLVDLAPTPDGVLVQPEAVALLAELRRLTNGAVAILSGRGIDSIDQFLGMPDLPIAGLH
GAERRDANGDTQRIGFNDERLLRMEQVLADIVNANPGMLLEIKGAALALHYRNAPDREPVAREATTRLVADYPEAYVLQP
GKMVYEIKPKDVDKGRALRAFLDEPPFTGRTPIFAGDDLTDEKGFAVVNDHGGLSIKVGSGDTIARSRIGSVSALLAWLS
EIVAAARSA
>Mature_249_residues
MQALPAFLSPTETAFFFDFDGTLVDLAPTPDGVLVQPEAVALLAELRRLTNGAVAILSGRGIDSIDQFLGMPDLPIAGLH
GAERRDANGDTQRIGFNDERLLRMEQVLADIVNANPGMLLEIKGAALALHYRNAPDREPVAREATTRLVADYPEAYVLQP
GKMVYEIKPKDVDKGRALRAFLDEPPFTGRTPIFAGDDLTDEKGFAVVNDHGGLSIKVGSGDTIARSRIGSVSALLAWLS
EIVAAARSA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=232, Percent_Identity=44.3965517241379, Blast_Score=194, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6320279, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920676, Length=245, Percent_Identity=28.9795918367347, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26721; Mature: 26721

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALPAFLSPTETAFFFDFDGTLVDLAPTPDGVLVQPEAVALLAELRRLTNGAVAILSGR
CCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEECHHHHHHHHHHHHHCCCEEEEEECC
GIDSIDQFLGMPDLPIAGLHGAERRDANGDTQRIGFNDERLLRMEQVLADIVNANPGMLL
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EIKGAALALHYRNAPDREPVAREATTRLVADYPEAYVLQPGKMVYEIKPKDVDKGRALRA
EECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHH
FLDEPPFTGRTPIFAGDDLTDEKGFAVVNDHGGLSIKVGSGDTIARSRIGSVSALLAWLS
HHCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
EIVAAARSA
HHHHHHHCC
>Mature Secondary Structure
MQALPAFLSPTETAFFFDFDGTLVDLAPTPDGVLVQPEAVALLAELRRLTNGAVAILSGR
CCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEECHHHHHHHHHHHHHCCCEEEEEECC
GIDSIDQFLGMPDLPIAGLHGAERRDANGDTQRIGFNDERLLRMEQVLADIVNANPGMLL
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEE
EIKGAALALHYRNAPDREPVAREATTRLVADYPEAYVLQPGKMVYEIKPKDVDKGRALRA
EECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCHHHHH
FLDEPPFTGRTPIFAGDDLTDEKGFAVVNDHGGLSIKVGSGDTIARSRIGSVSALLAWLS
HHCCCCCCCCCCEEECCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
EIVAAARSA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]