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The map label for this gene is otsA [H]

Identifier: 186475624

GI number: 186475624

Start: 957294

End: 958730

Strand: Direct

Name: otsA [H]

Synonym: Bphy_0859

Alternate gene names: 186475624

Gene position: 957294-958730 (Clockwise)

Preceding gene: 186475623

Following gene: 186475626

Centisome position: 27.51

GC content: 64.79

Gene sequence:

>1437_bases
ATGAGCCGATTGATCATCGTATCGAACCGCGTCGCGCCAATTTCGGAAGGCGGCCCGGCGGCGGGCGGCCTGGCCGTCGG
CGTATACGACGCATTGAAGGAAACGGGCGGCATGTGGTTTGGCTGGAGCGGCGAGGTGCTCGGGTCCGGCCAGCCGCAGA
TGAGGCTCGAAGAGCGCGGCCCCGTCACGTTCGCGACCATCGGCCTCGTGCGCCGCGATTACGACCAGTACTACCGCGGT
TTTTCGAACGCAACGCTGTGGCCCGCATTTCACTATCGGCCGGATCTGCTGCAGTTCGACCGGCACGATTTCGAAGGCTA
TTGCCGCGTGAATACGTGGCTCGCGCAACAGCTTGTGCCGCTACTACGCGACGACGACGTCATCTGGGTTCACGACTACC
ACCTGATTCCGTTCGCGCAGGCGTTGCGCGCGGCGGGCGTGAAGAACCGCATCGGCTTCTTTCTGCACATTCCGTTTCCG
GCGGCGCAGGTGCTGCTGGCCGTGCCGCCGCATCGCGCGCTGGTCGAGGCGCTTTGTTCGTTCGATCTGCTCGGCTTCCA
GACAAAGCCCGATTTACGGGCCTTCTGCGACTACATCGTCAACGAAGCGGATGGATGGGCGGAAGCACAGGCGCCGGGGC
GGAGCGCTGCGCCGACCGTCGTGCATGCGTTCGGCCGCACGCTGCGCGCGGCCGCATATCCGATCGGCGTGTATCCCGAC
GAGATAGCCGAGCTGGCGAAAGCGGGCGAGGGCGACAAGCCCGTGCGTACGATCGAGGCGACGCTGCATGCGCGCAAGCT
GATCATGAGCGTGGACCGGCTCGATTACTCGAAAGGGCTGGTCGAGCGCTTCCGCGCATTCGAGCGGCTGCTGGAGCACT
CGCCTTCTTATCGCGACAAGGTATCGTTCCTGCAGATCGCGCCGCCGACGCGTTCGGATCTACACGCGTATCAGGAAATC
AGATTGCAGCTGGAAGGCGAATCCGGGCGCATCAACGGACGCTTCGCGGAGCTCGACTGGACGCCGATCCGCTACATCCA
TCGGCAGTACGAGCGGCCCGTGCTGGCGGCGCTGTTCCGCACCGCGCACGTCGGCTACGTGACGCCGCTGCGCGACGGGA
TGAATCTCGTCGCGAAGGAATATGTGTCGGCTCAAGACCCGGAAGACCCCGGCGTGCTGGTGCTGTCGCGCTTTGCGGGC
GCTGCGCAGGAGCTGACGGGCGCGCTGATCGTCAATCCTGTCGATATCGACGGGATGGCCGACGCGCTCGGCACGGCGCT
GTCGATGCCGCTCGCCGAACGCAAGGCGCGCTACGTCGACATGATGGCGCAACTGCGTGAGAACAACGTGTCCGTGTGGC
GCGACAATTTCATGCGCGATCTGCAGCAGATGCCGGATCCGCTGGCGCAGTCGATCAGGGAAACGGCGCAAGGGTGA

Upstream 100 bases:

>100_bases
CTGCTCGCGTGGCTGTCCGAAATCGTCGCGGCGGCACGCAGCGCATGACCACGACCGTTCATCCGCGCCGTGCCCTTGTT
CAGCACGGAAGTCACGCAAC

Downstream 100 bases:

>100_bases
CGTCCCGCTTGCACGCCGATTAAAAAAGCCGCTTCGATCGAAGCGGCTTTTTTGTTGCGGCCGTGTCCGTCCAGACGGGC
GCGCCGTAGGTCACGCCACG

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGEVLGSGQPQMRLEERGPVTFATIGLVRRDYDQYYRG
FSNATLWPAFHYRPDLLQFDRHDFEGYCRVNTWLAQQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
AAQVLLAVPPHRALVEALCSFDLLGFQTKPDLRAFCDYIVNEADGWAEAQAPGRSAAPTVVHAFGRTLRAAAYPIGVYPD
EIAELAKAGEGDKPVRTIEATLHARKLIMSVDRLDYSKGLVERFRAFERLLEHSPSYRDKVSFLQIAPPTRSDLHAYQEI
RLQLEGESGRINGRFAELDWTPIRYIHRQYERPVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPEDPGVLVLSRFAG
AAQELTGALIVNPVDIDGMADALGTALSMPLAERKARYVDMMAQLRENNVSVWRDNFMRDLQQMPDPLAQSIRETAQG

Sequences:

>Translated_478_residues
MSRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGEVLGSGQPQMRLEERGPVTFATIGLVRRDYDQYYRG
FSNATLWPAFHYRPDLLQFDRHDFEGYCRVNTWLAQQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFP
AAQVLLAVPPHRALVEALCSFDLLGFQTKPDLRAFCDYIVNEADGWAEAQAPGRSAAPTVVHAFGRTLRAAAYPIGVYPD
EIAELAKAGEGDKPVRTIEATLHARKLIMSVDRLDYSKGLVERFRAFERLLEHSPSYRDKVSFLQIAPPTRSDLHAYQEI
RLQLEGESGRINGRFAELDWTPIRYIHRQYERPVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPEDPGVLVLSRFAG
AAQELTGALIVNPVDIDGMADALGTALSMPLAERKARYVDMMAQLRENNVSVWRDNFMRDLQQMPDPLAQSIRETAQG
>Mature_477_residues
SRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGEVLGSGQPQMRLEERGPVTFATIGLVRRDYDQYYRGF
SNATLWPAFHYRPDLLQFDRHDFEGYCRVNTWLAQQLVPLLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPA
AQVLLAVPPHRALVEALCSFDLLGFQTKPDLRAFCDYIVNEADGWAEAQAPGRSAAPTVVHAFGRTLRAAAYPIGVYPDE
IAELAKAGEGDKPVRTIEATLHARKLIMSVDRLDYSKGLVERFRAFERLLEHSPSYRDKVSFLQIAPPTRSDLHAYQEIR
LQLEGESGRINGRFAELDWTPIRYIHRQYERPVLAALFRTAHVGYVTPLRDGMNLVAKEYVSAQDPEDPGVLVLSRFAGA
AQELTGALIVNPVDIDGMADALGTALSMPLAERKARYVDMMAQLRENNVSVWRDNFMRDLQQMPDPLAQSIRETAQG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=464, Percent_Identity=53.2327586206897, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI115533560, Length=357, Percent_Identity=31.9327731092437, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115533558, Length=357, Percent_Identity=31.9327731092437, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71987012, Length=435, Percent_Identity=28.0459770114943, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319602, Length=475, Percent_Identity=32.2105263157895, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6320279, Length=420, Percent_Identity=26.6666666666667, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323537, Length=397, Percent_Identity=27.455919395466, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323917, Length=400, Percent_Identity=26.75, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19920676, Length=491, Percent_Identity=34.826883910387, Blast_Score=274, Evalue=8e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53461; Mature: 53329

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGEVLGSGQPQMRLEERG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEECCCC
PVTFATIGLVRRDYDQYYRGFSNATLWPAFHYRPDLLQFDRHDFEGYCRVNTWLAQQLVP
CEEEEHHHHHHHHHHHHHCCCCCCEECCEECCCCCHHHHCCCCCCCEEEHHHHHHHHHHH
LLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPAAQVLLAVPPHRALVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHH
FDLLGFQTKPDLRAFCDYIVNEADGWAEAQAPGRSAAPTVVHAFGRTLRAAAYPIGVYPD
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
EIAELAKAGEGDKPVRTIEATLHARKLIMSVDRLDYSKGLVERFRAFERLLEHSPSYRDK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSFLQIAPPTRSDLHAYQEIRLQLEGESGRINGRFAELDWTPIRYIHRQYERPVLAALFR
CCEEEECCCCHHHHHHHHHHEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHH
TAHVGYVTPLRDGMNLVAKEYVSAQDPEDPGVLVLSRFAGAAQELTGALIVNPVDIDGMA
HHHCCEECCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCCCHHH
DALGTALSMPLAERKARYVDMMAQLRENNVSVWRDNFMRDLQQMPDPLAQSIRETAQG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLIIVSNRVAPISEGGPAAGGLAVGVYDALKETGGMWFGWSGEVLGSGQPQMRLEERG
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEECCCC
PVTFATIGLVRRDYDQYYRGFSNATLWPAFHYRPDLLQFDRHDFEGYCRVNTWLAQQLVP
CEEEEHHHHHHHHHHHHHCCCCCCEECCEECCCCCHHHHCCCCCCCEEEHHHHHHHHHHH
LLRDDDVIWVHDYHLIPFAQALRAAGVKNRIGFFLHIPFPAAQVLLAVPPHRALVEALCS
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHHHH
FDLLGFQTKPDLRAFCDYIVNEADGWAEAQAPGRSAAPTVVHAFGRTLRAAAYPIGVYPD
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
EIAELAKAGEGDKPVRTIEATLHARKLIMSVDRLDYSKGLVERFRAFERLLEHSPSYRDK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSFLQIAPPTRSDLHAYQEIRLQLEGESGRINGRFAELDWTPIRYIHRQYERPVLAALFR
CCEEEECCCCHHHHHHHHHHEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHH
TAHVGYVTPLRDGMNLVAKEYVSAQDPEDPGVLVLSRFAGAAQELTGALIVNPVDIDGMA
HHHCCEECCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCHHHHCCCEEEECCCCCCHHH
DALGTALSMPLAERKARYVDMMAQLRENNVSVWRDNFMRDLQQMPDPLAQSIRETAQG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA