Click here to switch to the map view.

The map label for this gene is algU [H]

Identifier: 186475599

GI number: 186475599

Start: 932864

End: 933463

Strand: Direct

Name: algU [H]

Synonym: Bphy_0834

Alternate gene names: 186475599

Gene position: 932864-933463 (Clockwise)

Preceding gene: 186475598

Following gene: 186475600

Centisome position: 26.81

GC content: 57.17

Gene sequence:

>600_bases
GTGAGCGAAAAAGAAATAGATCAGGTGCTGGTTGAACGCGTTCAGAACGGCGACAAGGCCGCGTTCGAGCTTCTGGTCTC
AAAGTACCACCGCAAGATCATCCGGCTGATCTCGCGCCTCGTGCGGGATCCTGCCGAGGTCGAGGACGTGGCCCAGGATG
CCTTCATCAAGGCATACCGGGCGCTGCCCCAATTCCGCGGCGAGTCCGCGTTCTATACGTGGTTGTACCGTATTGCCGTC
AATACGGCCAAGAATTACCTTGCGACCCAAGGCCGGCGAGCGCCCACTTCGACGGAAGCAGATGCTGAAGAAGCGGAAAC
TTTCTCGGATGCCGACCAACTAAGGGATATCAACACGCCCGAGTCGATGTTGATGAGCAAACAGATCGCTGAGACGGTCA
ATGCTGCGATGGCGGTTTTACCGGAAGAGCTGCGCACCGCCATAACTCTTCGTGAAATTGAAGGTCTGAGCTACGAGGAA
ATCGCCGAGATGATGGGCTGCCCAATCGGCACCGTCAGATCGAGAATTTTCCGCGCTCGCGAGGCCATTGCGGCAAAATT
GCGTCCGCTGCTTGACACACCCGAAGGCAAGCGCTGGTAG

Upstream 100 bases:

>100_bases
TGTAAAGACTGTAACGGGTGTTACGGGCGTAACGTGCCGAGGTGAAGGGGACGCTACAATGGTGCCCCGCTACACGCGCA
AAAGTTAACGGATTTATCAG

Downstream 100 bases:

>100_bases
CGGCTGTTGGACAGCGGCCAGCCGGCCTCGCGGACAGCGGCGCAGTATCTGGGTCAAGTGGTGTCACTACGGGATGTCTG
AAAGATGGGGAGCATCATGG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSEKEIDQVLVERVQNGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Sequences:

>Translated_199_residues
MSEKEIDQVLVERVQNGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAV
NTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEE
IAEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW
>Mature_198_residues
SEKEIDQVLVERVQNGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVN
TAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTPESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEI
AEMMGCPIGTVRSRIFRAREAIAAKLRPLLDTPEGKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=61.2565445026178, Blast_Score=235, Evalue=1e-63,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22567; Mature: 22436

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKEIDQVLVERVQNGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEKEIDQVLVERVQNGDKAAFELLVSKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRAPTSTEADAEEAETFSDADQLRDINTP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCH
ESMLMSKQIAETVNAAMAVLPEELRTAITLREIEGLSYEEIAEMMGCPIGTVRSRIFRAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
EAIAAKLRPLLDTPEGKRW
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]