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The map label for this gene is dfrA [H]

Identifier: 186475547

GI number: 186475547

Start: 876873

End: 877373

Strand: Reverse

Name: dfrA [H]

Synonym: Bphy_0782

Alternate gene names: 186475547

Gene position: 877373-876873 (Counterclockwise)

Preceding gene: 186475548

Following gene: 186475545

Centisome position: 25.22

GC content: 61.68

Gene sequence:

>501_bases
ATGACGACGCTCACCCTGATCGTCGCTCGCGCCCGCAATGGCGTGATCGGCCGTGACAACCAGCTGCCCTGGCGGCTTCC
CGAAGACCTCGCTTTCTTCAAGCGCACGACGATGGGCGCACCCATCGTGATGGGGCGCAAGACGCATGAATCAATTGGCC
GTGTGTTGCCGGGGCGGCGCAATATCGTCGTGACGCGCGATGCGCAGCGCCGCTTCGAGGGCTGCGATACCGTGACGAAT
CTCGACGACGCGTTGGCGCTCGCCGCGCGCGATGGTGCGGGCGAGGCGTTTTTGATAGGCGGTGCGCAGCTGTATGAGGA
AGGGTTGCGGCGCGCGGACAAAATGATCGTGACTGAAATTCACGCCGATTTCGATGGCGATGCCTGGTTTCCTGCGCCTG
ATGTGGCGCACTGGCAAGAGGTCTCGCGGGAGACGCATCAGGCCAAAGAGCCCAATGACTTTGAATATGCCTTTGTGGTT
TATCGGCGCCTTGCCACTTGA

Upstream 100 bases:

>100_bases
TTTCGGCTCGCGTTTCGGCAAGCGTTCGGCTGGACGGCAGGCTGTCGGGTAGAATCGGCGTTCGCTTTCGTACCCTGCTC
CCTACCGCTTTTTCATTCGC

Downstream 100 bases:

>100_bases
GGTTGATGGGTATTTTTGTCGGCGACGCTTCTAGTTGGTTGGTTTTTGGTTTTTTTTGCCGGCATCCGCGTTTTGCCTTC
GTACTCATGCGTTGCCCCTG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTTLTLIVARARNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIVMGRKTHESIGRVLPGRRNIVVTRDAQRRFEGCDTVTN
LDDALALAARDGAGEAFLIGGAQLYEEGLRRADKMIVTEIHADFDGDAWFPAPDVAHWQEVSRETHQAKEPNDFEYAFVV
YRRLAT

Sequences:

>Translated_166_residues
MTTLTLIVARARNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIVMGRKTHESIGRVLPGRRNIVVTRDAQRRFEGCDTVTN
LDDALALAARDGAGEAFLIGGAQLYEEGLRRADKMIVTEIHADFDGDAWFPAPDVAHWQEVSRETHQAKEPNDFEYAFVV
YRRLAT
>Mature_165_residues
TTLTLIVARARNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIVMGRKTHESIGRVLPGRRNIVVTRDAQRRFEGCDTVTNL
DDALALAARDGAGEAFLIGGAQLYEEGLRRADKMIVTEIHADFDGDAWFPAPDVAHWQEVSRETHQAKEPNDFEYAFVVY
RRLAT

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=182, Percent_Identity=29.6703296703297, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1786233, Length=164, Percent_Identity=40.8536585365854, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17505899, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116008026, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647458, Length=182, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18668; Mature: 18537

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTLIVARARNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIVMGRKTHESIGRVLPGRR
CCHHEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCC
NIVVTRDAQRRFEGCDTVTNLDDALALAARDGAGEAFLIGGAQLYEEGLRRADKMIVTEI
CEEEECCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHE
HADFDGDAWFPAPDVAHWQEVSRETHQAKEPNDFEYAFVVYRRLAT
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTLTLIVARARNGVIGRDNQLPWRLPEDLAFFKRTTMGAPIVMGRKTHESIGRVLPGRR
CHHEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCC
NIVVTRDAQRRFEGCDTVTNLDDALALAARDGAGEAFLIGGAQLYEEGLRRADKMIVTEI
CEEEECCHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHE
HADFDGDAWFPAPDVAHWQEVSRETHQAKEPNDFEYAFVVYRRLAT
ECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]