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The map label for this gene is pmbA [H]

Identifier: 186475546

GI number: 186475546

Start: 875311

End: 876681

Strand: Direct

Name: pmbA [H]

Synonym: Bphy_0781

Alternate gene names: 186475546

Gene position: 875311-876681 (Clockwise)

Preceding gene: 186475543

Following gene: 186475552

Centisome position: 25.16

GC content: 63.6

Gene sequence:

>1371_bases
ATGGCAGCAGACACGGAAGCCAGGCAACGCTTTTTCCCGCACACGCAGGACGAGCTGAAAGAAATCGCCTCCGACATTCT
TCGTCACGCAAAGGCGCTCGGCGCAACCGACGCGGCCACCGAAATCTCGGAAGGCGACGGGCTGTCCGTCTCCGTGCGCC
GCGGCGAAGTGGAGACGATCGAGCACAACCGCGACAAGATGGTCGGCGTGACGGTCTTCATTGGCAACAAGCGAGGTAAT
GCAAGCACGTCCGACTTTTCGTCCGAGGCGCTGAAGGACACGGTCATGGCCGCGTACAACATCGCGCGCTTCACGGCCGA
AGACGACTGCGCGGGTCTTGCGGAAGAAGAATTGCTCGAAACCCACCCGCGCGATCTGGATCTGTATCACCCGTGGAATC
TGGACGCCGACGAAGCCGTCGAACTGGCTCGGCGCGCAGAAGATGCCGCGTTCGCGACGAATCCGATGATCAAGAATTCG
GAGGGCGCGAGCGTATCGGCGCAGCATTCGCAGTTTGTGCTCGGCACGTCGCGCGGATTCCTGGCGGGTTATCCGTATTC
GCGTCACTACATCGCGTGTGCGCCGATCGCGGGCAGCGGCCGCAACATGCAGCGCGACGACTGGTACACGTCGAAGCGCA
GCGCGGGCGAGCTCGCCGATCCCGAAGCCGTGGGGCGCTATGCCGCCGAGCGCGCATTGGCGCGCATGGGCGCGCGCAGT
CTGGACACGCGCAAGGTGCCCGTGCTGTTCGAAGCGCCGCTCGCGGCGGGACTGCTCGGCGCGTTCGTGCAGGCGACGAG
CGGCGGCGCGCTGTATCGCAAGACATCATTCCTCGTCGACAGTCTGGGTAAGCCCGTATTCGCGCCGCATGTGCAGGTGG
TCGAAGATCCGCACGTGCCGCGCGCGATGGGCAGCGCGCCGTTCGACGAAGAGGGCGTGCGCACAAAGCAGCGTTCGGTG
GTGAAGGACGGCGTCGTCGAGGGCTATTTCCTGTCCACGTACTCGGCGCGCAAGCTCGGCATGCCGACGACGGGTAACGC
CGGCGGTTCGCATAACCTTTCGCTGCGCAGTTCGTTGACGCAGCCTACCGATGACTTCGAAGAGATGTTGCGCAAGCTGG
GCACCGGTTTGTTGCTGACCGAATTGATGGGGCAGGGTGTCAACTACGTGACGGGCGATTATTCGCGCGGCGCGTCCGGG
TTCTGGGTCGAGAATGGCAAGATCCAGTATCCCGTCGAGGAGATCACCGTGGCGAGCACGTTGCAGGAGATGTTCCGGCA
TGTCGTCGCGATTGGGGCGGACACGATTGTGCGCGGGACCAAGCAGACCGGTTCTGTGCTGATTGAACGGATGACTGTCG
CTGGGCAGTGA

Upstream 100 bases:

>100_bases
ATACGTCGGCGCACAGGCCGGCTGGATACAGCGCTTGCAACGACGAACTCTCGCGGGCTTCGGCCCGGACGGCACCGCAA
ACTCAGGACGGCAAACGACA

Downstream 100 bases:

>100_bases
TCTCGTTTTAGCCGGCGGCTGGCCTTCGATTTGTGAGCCTCTTTTCGCTGGTATCCGCGTTTCGTTTCTGGTTTGCTGGC
GTTGCCCCGCACAGGGGCAA

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAADTEARQRFFPHTQDELKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGN
ASTSDFSSEALKDTVMAAYNIARFTAEDDCAGLAEEELLETHPRDLDLYHPWNLDADEAVELARRAEDAAFATNPMIKNS
EGASVSAQHSQFVLGTSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSKRSAGELADPEAVGRYAAERALARMGARS
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTSFLVDSLGKPVFAPHVQVVEDPHVPRAMGSAPFDEEGVRTKQRSV
VKDGVVEGYFLSTYSARKLGMPTTGNAGGSHNLSLRSSLTQPTDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASG
FWVENGKIQYPVEEITVASTLQEMFRHVVAIGADTIVRGTKQTGSVLIERMTVAGQ

Sequences:

>Translated_456_residues
MAADTEARQRFFPHTQDELKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGN
ASTSDFSSEALKDTVMAAYNIARFTAEDDCAGLAEEELLETHPRDLDLYHPWNLDADEAVELARRAEDAAFATNPMIKNS
EGASVSAQHSQFVLGTSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSKRSAGELADPEAVGRYAAERALARMGARS
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTSFLVDSLGKPVFAPHVQVVEDPHVPRAMGSAPFDEEGVRTKQRSV
VKDGVVEGYFLSTYSARKLGMPTTGNAGGSHNLSLRSSLTQPTDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASG
FWVENGKIQYPVEEITVASTLQEMFRHVVAIGADTIVRGTKQTGSVLIERMTVAGQ
>Mature_455_residues
AADTEARQRFFPHTQDELKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGNA
STSDFSSEALKDTVMAAYNIARFTAEDDCAGLAEEELLETHPRDLDLYHPWNLDADEAVELARRAEDAAFATNPMIKNSE
GASVSAQHSQFVLGTSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSKRSAGELADPEAVGRYAAERALARMGARSL
DTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTSFLVDSLGKPVFAPHVQVVEDPHVPRAMGSAPFDEEGVRTKQRSVV
KDGVVEGYFLSTYSARKLGMPTTGNAGGSHNLSLRSSLTQPTDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGF
WVENGKIQYPVEEITVASTLQEMFRHVVAIGADTIVRGTKQTGSVLIERMTVAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=47.1526195899772, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1789640, Length=395, Percent_Identity=22.5316455696203, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49333; Mature: 49202

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADTEARQRFFPHTQDELKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGNKRGNASTSDFSSEALKDTVMAAYNIARFTAEDDCAGLAEEELLE
HCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHH
THPRDLDLYHPWNLDADEAVELARRAEDAAFATNPMIKNSEGASVSAQHSQFVLGTSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCCEEEEECCCCC
LAGYPYSRHYIACAPIAGSGRNMQRDDWYTSKRSAGELADPEAVGRYAAERALARMGARS
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTSFLVDSLGKPVFAPHVQVVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
RAMGSAPFDEEGVRTKQRSVVKDGVVEGYFLSTYSARKLGMPTTGNAGGSHNLSLRSSLT
HHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCCCCCCCHHHHHCC
QPTDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGFWVENGKIQYPVEEITVAST
CCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHVVAIGADTIVRGTKQTGSVLIERMTVAGQ
HHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AADTEARQRFFPHTQDELKEIASDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGNKRGNASTSDFSSEALKDTVMAAYNIARFTAEDDCAGLAEEELLE
HCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHH
THPRDLDLYHPWNLDADEAVELARRAEDAAFATNPMIKNSEGASVSAQHSQFVLGTSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCCEEEEECCCCC
LAGYPYSRHYIACAPIAGSGRNMQRDDWYTSKRSAGELADPEAVGRYAAERALARMGARS
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTSFLVDSLGKPVFAPHVQVVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCC
RAMGSAPFDEEGVRTKQRSVVKDGVVEGYFLSTYSARKLGMPTTGNAGGSHNLSLRSSLT
HHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCCCCCCCHHHHHCC
QPTDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGFWVENGKIQYPVEEITVAST
CCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHVVAIGADTIVRGTKQTGSVLIERMTVAGQ
HHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]