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The map label for this gene is paaF [C]

Identifier: 186475522

GI number: 186475522

Start: 853815

End: 854624

Strand: Direct

Name: paaF [C]

Synonym: Bphy_0757

Alternate gene names: 186475522

Gene position: 853815-854624 (Clockwise)

Preceding gene: 186475521

Following gene: 186475524

Centisome position: 24.54

GC content: 64.81

Gene sequence:

>810_bases
ATGAATGCAGAGGCGACAGGCGCACTGATCGAACGCACGCGAGACGCGTATGACCTCGGCGGGGTGGTGCGCCTCACGTT
GAACCGGCCGGACGCGTTCAATGCTCTTTCCGAGGCGCTGCTCGATGAGCTTCAGATGCATCTTGCAGACATCGCGAAGA
GCGATGCGCGCGTGGTCGTGATCGCGGGTGCGGGCCGCGCGTTTTGTGCAGGACATGATCTGCGTGAAATGCGCGCGGCG
CCGTCGCTCGATTACTACCAGTCGCTGTTCGCGCGCTGCACAAAAATGATGCTTGCGATCCAGCGGCTGCCGCAGCCGGT
GATCGCTCGCGTGCACGGCATTGCGACGGCGGCGGGCTGTCAGCTCGTCGCGATGTGCGATCTCGCGGTCGCCGCCGATA
CAGCGCGCTTCGCGGTGTCGGGAGTGAATCTCGGGCTCTTCTGTGCGACGCCGTCCGTGCCGCTGTCGCGCAACCTGTCG
CGCAAGGCTGCGCTCGACATGCTGCTGACGGGCGATTTCATCGACGCGATGGCCGCGAAGCAGCAAGGTCTCGTGAATCG
CGTGGTGCCGATGGACGCGCTCGACAACGAAGTCGCCGCGCTCGCGAAGAGCATCTGCGCGAAGCCACGCGAGGCCATCG
AAGCGGGCAAGGGTCTTTTCTACCGGCAACTCGAAATGGGCATCGAAGCCGCGTATCAACTGGCCGGGCAAACGATGGCG
TGCAACATGATGGACGAGTCGGCGCTCGAAGGCGTGCAGGCGTTCATCGACAAGCGGCCGCCCGACTGGAAACCCGCGCG
GGGCGGCTGA

Upstream 100 bases:

>100_bases
GCGGCTACAACCTGTCGGCGCTCGGACGCAGCGTCGTCGCGCACGTAAGGGCGCTCGCGGATATCTGAGCGCGAGGGCGT
GCATCGCAGGAGGCGGCATC

Downstream 100 bases:

>100_bases
ACGCGTCCATCAGCCTCGCTCCGAACGCGCCACTATCCACGCGACGAGTGCGTCGATCACGCGTTCGCGGTCGAGGTCGT
TCATCGTCTCGTGATAACTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNAEATGALIERTRDAYDLGGVVRLTLNRPDAFNALSEALLDELQMHLADIAKSDARVVVIAGAGRAFCAGHDLREMRAA
PSLDYYQSLFARCTKMMLAIQRLPQPVIARVHGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLS
RKAALDMLLTGDFIDAMAAKQQGLVNRVVPMDALDNEVAALAKSICAKPREAIEAGKGLFYRQLEMGIEAAYQLAGQTMA
CNMMDESALEGVQAFIDKRPPDWKPARGG

Sequences:

>Translated_269_residues
MNAEATGALIERTRDAYDLGGVVRLTLNRPDAFNALSEALLDELQMHLADIAKSDARVVVIAGAGRAFCAGHDLREMRAA
PSLDYYQSLFARCTKMMLAIQRLPQPVIARVHGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLS
RKAALDMLLTGDFIDAMAAKQQGLVNRVVPMDALDNEVAALAKSICAKPREAIEAGKGLFYRQLEMGIEAAYQLAGQTMA
CNMMDESALEGVQAFIDKRPPDWKPARGG
>Mature_269_residues
MNAEATGALIERTRDAYDLGGVVRLTLNRPDAFNALSEALLDELQMHLADIAKSDARVVVIAGAGRAFCAGHDLREMRAA
PSLDYYQSLFARCTKMMLAIQRLPQPVIARVHGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLS
RKAALDMLLTGDFIDAMAAKQQGLVNRVVPMDALDNEVAALAKSICAKPREAIEAGKGLFYRQLEMGIEAAYQLAGQTMA
CNMMDESALEGVQAFIDKRPPDWKPARGG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=43.4782608695652, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=31.3953488372093, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=29.6442687747036, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=23.4817813765182, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=247, Percent_Identity=23.4817813765182, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=247, Percent_Identity=23.4817813765182, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=27.3809523809524, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI20127408, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=36.144578313253, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=32.5490196078431, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=30.7984790874525, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=29.9610894941634, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=252, Percent_Identity=41.6666666666667, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=32, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=28.8617886178862, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=28.968253968254, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=233, Percent_Identity=29.1845493562232, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=193, Percent_Identity=28.4974093264249, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=37.8048780487805, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=31.1203319502075, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=31.1203319502075, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=28.6245353159851, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=27.3542600896861, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357171, Length=222, Percent_Identity=27.9279279279279, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24650670, Length=272, Percent_Identity=25.7352941176471, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28771; Mature: 28771

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEATGALIERTRDAYDLGGVVRLTLNRPDAFNALSEALLDELQMHLADIAKSDARVVV
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IAGAGRAFCAGHDLREMRAAPSLDYYQSLFARCTKMMLAIQRLPQPVIARVHGIATAAGC
EECCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLSRKAALDMLLTGDFIDAMAAK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QQGLVNRVVPMDALDNEVAALAKSICAKPREAIEAGKGLFYRQLEMGIEAAYQLAGQTMA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHE
CNMMDESALEGVQAFIDKRPPDWKPARGG
EHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNAEATGALIERTRDAYDLGGVVRLTLNRPDAFNALSEALLDELQMHLADIAKSDARVVV
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IAGAGRAFCAGHDLREMRAAPSLDYYQSLFARCTKMMLAIQRLPQPVIARVHGIATAAGC
EECCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLSRKAALDMLLTGDFIDAMAAK
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QQGLVNRVVPMDALDNEVAALAKSICAKPREAIEAGKGLFYRQLEMGIEAAYQLAGQTMA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHE
CNMMDESALEGVQAFIDKRPPDWKPARGG
EHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA