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The map label for this gene is gph [C]

Identifier: 186475500

GI number: 186475500

Start: 829187

End: 829900

Strand: Reverse

Name: gph [C]

Synonym: Bphy_0735

Alternate gene names: 186475500

Gene position: 829900-829187 (Counterclockwise)

Preceding gene: 186475501

Following gene: 186475499

Centisome position: 23.85

GC content: 65.41

Gene sequence:

>714_bases
ATGAGCGATCCCACGCCCCTGCCTCAGCGCGAAGACGAAGAAGCATCGATCGGCCTTTGCCAGGGCATTCTGTTCGACCT
GGACGGCACGCTGGCCGATACCGCGCCCGACCTGGCCGCAGCCGTCAACAGGATGCGTCACGAGCGAGGCCTCGAGATGG
TGCCGTTGGAACAGTTGCGGCCGCTCGCGTCGGCGGGCGCGCGCGGCCTGCTGGGTGGCGCGTTTGGCATCGGACCGGAG
CATCACGACTATGCGTCGATGCGCGAAGAGTTCCTCGCCAACTATGAAGCCGATCTCTGCATCGAGACGATTCTGTTCCC
TGGAATCGGCGAGATTCTCGACGACCTCGACGTGCGCGGCGTCCGCTGGGGCATCGTGACGAACAAGGTCGCCCGGCTGA
CGGAGCCGCTCATCGCTCAACTGGGGCTGGATACGCGTGCGGGCTGCGTCGTCAGCGGAGACACGACGCCGCATTCAAAG
CCTCATCCCGCGCCGCTCCTGCACGCAGCGAAGGAGCTGGATTTGCCGCCGGAGCGGATCGTCTATGTCGGCGACGACCT
GCGCGACGTGCAGGCCGGCTTCGCAGCCGGAATGGTGACGGTCGCGGCAGCATACGGTTACTGCGGCAACGACATTCCGC
CCACGCAGTGGCACGCGAAGCACGTCGTCGGTTCCACAGCCGAACTCCAGAATCTGCTGCGCGACATCAGTTGA

Upstream 100 bases:

>100_bases
GATTTCGGCCTGTCGAGCGACACCGATGTGAACTACATGCTCGCGTGCCGCCGCGAAGCCTGATCCATTTTTTTGCGCCC
GATTAACTGCATCTACTTCA

Downstream 100 bases:

>100_bases
CGCACGAACGCGACGCCGGCACCACGCGAATGTTTCGTCGTGACTCCGGCGGGCCGCTATTGTAAAATGCCTTCTTGGGC
CACATATATGGCTCATTCCT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSDPTPLPQREDEEASIGLCQGILFDLDGTLADTAPDLAAAVNRMRHERGLEMVPLEQLRPLASAGARGLLGGAFGIGPE
HHDYASMREEFLANYEADLCIETILFPGIGEILDDLDVRGVRWGIVTNKVARLTEPLIAQLGLDTRAGCVVSGDTTPHSK
PHPAPLLHAAKELDLPPERIVYVGDDLRDVQAGFAAGMVTVAAAYGYCGNDIPPTQWHAKHVVGSTAELQNLLRDIS

Sequences:

>Translated_237_residues
MSDPTPLPQREDEEASIGLCQGILFDLDGTLADTAPDLAAAVNRMRHERGLEMVPLEQLRPLASAGARGLLGGAFGIGPE
HHDYASMREEFLANYEADLCIETILFPGIGEILDDLDVRGVRWGIVTNKVARLTEPLIAQLGLDTRAGCVVSGDTTPHSK
PHPAPLLHAAKELDLPPERIVYVGDDLRDVQAGFAAGMVTVAAAYGYCGNDIPPTQWHAKHVVGSTAELQNLLRDIS
>Mature_236_residues
SDPTPLPQREDEEASIGLCQGILFDLDGTLADTAPDLAAAVNRMRHERGLEMVPLEQLRPLASAGARGLLGGAFGIGPEH
HDYASMREEFLANYEADLCIETILFPGIGEILDDLDVRGVRWGIVTNKVARLTEPLIAQLGLDTRAGCVVSGDTTPHSKP
HPAPLLHAAKELDLPPERIVYVGDDLRDVQAGFAAGMVTVAAAYGYCGNDIPPTQWHAKHVVGSTAELQNLLRDIS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=208, Percent_Identity=32.2115384615385, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25446; Mature: 25315

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPTPLPQREDEEASIGLCQGILFDLDGTLADTAPDLAAAVNRMRHERGLEMVPLEQLR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
PLASAGARGLLGGAFGIGPEHHDYASMREEFLANYEADLCIETILFPGIGEILDDLDVRG
HHHHHCCCHHCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCC
VRWGIVTNKVARLTEPLIAQLGLDTRAGCVVSGDTTPHSKPHPAPLLHAAKELDLPPERI
EEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHE
VYVGDDLRDVQAGFAAGMVTVAAAYGYCGNDIPPTQWHAKHVVGSTAELQNLLRDIS
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SDPTPLPQREDEEASIGLCQGILFDLDGTLADTAPDLAAAVNRMRHERGLEMVPLEQLR
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHH
PLASAGARGLLGGAFGIGPEHHDYASMREEFLANYEADLCIETILFPGIGEILDDLDVRG
HHHHHCCCHHCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCC
VRWGIVTNKVARLTEPLIAQLGLDTRAGCVVSGDTTPHSKPHPAPLLHAAKELDLPPERI
EEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHE
VYVGDDLRDVQAGFAAGMVTVAAAYGYCGNDIPPTQWHAKHVVGSTAELQNLLRDIS
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA