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The map label for this gene is pcpB [H]

Identifier: 186475490

GI number: 186475490

Start: 817568

End: 819283

Strand: Direct

Name: pcpB [H]

Synonym: Bphy_0725

Alternate gene names: 186475490

Gene position: 817568-819283 (Clockwise)

Preceding gene: 186475489

Following gene: 186475495

Centisome position: 23.5

GC content: 66.84

Gene sequence:

>1716_bases
ATGACGGGTGCTTCCTTCGACGTACCGCCCGTGCTGATCGTCGGCGCGGGGCCGACGGGGCTGGCGGCAGCCATGAGTCT
TGCGCGCGCGCACGTGCCCGTGCGGCTCATCGACAAGGCGCCGCAGGCAGATCCGCATTCACGCGCGATCGGCATCCAGG
CGCGCACGCTCGAACTGCTTGAACAACACCGGATCGTGGACCGGTTTCTGGAGCTCGGCCATCGCGCGCGCACCGCGAAC
CTGTACTCGAACGGGCATCGGCTCGCGCGGCTCGACTTCGATCCGTTGCAGACGCGCTATCCCTATCTGCTGTTTCTCGA
GCAGTCCGTCACCGAGCACCTGTTGACGGAGCACCTCGCCACGTTCGGCGTGCAGGTCGAACGCGGCACCGAGCTGACGA
TGTTCGCGCAAGGTTCGGCGGGCATCAACGCGACGCTGCAACGCCTGGACGGCCATGTCGAAACGCTCCATCCGTCGTAC
ATGATCGCCGCCGACGGCGCGCATAGCGCGATCCGCCACCGGCTCGGCATGAGCTTCGCGGGCAAGACGTTCGAGCAGAC
TTTCCTTCTCGCCGATCTCGAAGCCGATACCGGCTGGTCCGACGATGAATTCCATATCTTCGCTTCGGGTGCGGGGCTCG
CGGCGCTCTTCCCGATGGGCAAAGGCCGGCATCGGCTGATCGCCGATCATCCCGCGATGTCTCCCTCCGTGCCGTCAACG
GGCGACGCTGCGCCGCTCGCGGCGATTCCCGCGCCGACGCTCGACGAATGCCGTCTGATCGCGAAGAGCCGCATCCATCA
CCCCATCGACATCACGGGCCTGAGCTGGTCGGGCTATTTTCATCTGAATAGCCGCATGGTCGAGCAGTTGCGCGCGCAGC
GCGTGTTCCTGGCGGGCGATGCCGCGCACGTGCATAGTCCCGCCGGCGCGCAGGGGATGAATACAGGCATACAGGAGGCG
TTCAATCTCGGCTGGAAGATTGCACGCGTGCTGAAGGGCGATGCGCCCGAGCGGCTGCTCGACACATACCACCTCGAACG
TCATCCGATCGAGCGCGACGTGCTGCGGCAAACGAGCTTCGTCACGCAACTGGCGGAAGCGGACCACGGCCCGCTCAAGC
TGCTGCGCGAGCGCGTGATGCCTGTGCTCGCTGCGCTGGGACCGTTGCGCGATGCCGCGCGGCTCACGGTCAGCGAACTG
GCGATTCAATACCGGCGCTCGCCGCTGACGCTCGAACGCGTGCTCGACGGCGGCCCCCGTGCGGGCGAGCGTGCACCCGA
TGCGCTCGTGCACGTCGTCGATGGGCCGCTGGGACGTGCGCCCGGCACAGGCTGCATTTTCGATCTGCATGACCCGGCGT
TCTTCTCGCTTTTTTTGCTCGTCGATCCACCCGAAGAAGCGGCGTCCAGCAACGGCAAGCTGCTGCGCCTCGCGCGCAAG
CCCGTGCGATCCGCCGATCTCGACCGGTTCGTCTGCGCGGTCGAAGGGCTGCTGCCGGGCGCAGTGCGCGTGTGGCGCGT
GTCCGATGCGAGCGGCGACGGCGGCGGGCCATCGCTCAGCGAATCATTCGGCCGTACGCGGCCTTCGTTCTATCTGGTGC
GGCCCGACGGCTACGTCTGCGCACGCGGACGACCGGGCTCCGATCTGAACGGGCTGCTGCGTCATTGCGAAGCGTGGTTC
GCAAAAGGCGCAATAGCGGAGCCTCCGCCGCAGTGA

Upstream 100 bases:

>100_bases
ATCTTCCGATGACGCGTTGATGCGCTGCGTCGTCGGCCGCGCGGCAGCGACGCAAATGCTCGGCGTACACTAGCGGACAT
GTCCGACTGCGGAGGTCTGC

Downstream 100 bases:

>100_bases
CGTGGCCATAAAAAAAAAGCGTCCGCATCGTTGCCGATGCGAACGCCAAGGTGCTGTTGCGTGGATAGAACGCGTTATGC
GGCGGCCGGCTCCTTCGGCG

Product: FAD-binding monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTGASFDVPPVLIVGAGPTGLAAAMSLARAHVPVRLIDKAPQADPHSRAIGIQARTLELLEQHRIVDRFLELGHRARTAN
LYSNGHRLARLDFDPLQTRYPYLLFLEQSVTEHLLTEHLATFGVQVERGTELTMFAQGSAGINATLQRLDGHVETLHPSY
MIAADGAHSAIRHRLGMSFAGKTFEQTFLLADLEADTGWSDDEFHIFASGAGLAALFPMGKGRHRLIADHPAMSPSVPST
GDAAPLAAIPAPTLDECRLIAKSRIHHPIDITGLSWSGYFHLNSRMVEQLRAQRVFLAGDAAHVHSPAGAQGMNTGIQEA
FNLGWKIARVLKGDAPERLLDTYHLERHPIERDVLRQTSFVTQLAEADHGPLKLLRERVMPVLAALGPLRDAARLTVSEL
AIQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGTGCIFDLHDPAFFSLFLLVDPPEEAASSNGKLLRLARK
PVRSADLDRFVCAVEGLLPGAVRVWRVSDASGDGGGPSLSESFGRTRPSFYLVRPDGYVCARGRPGSDLNGLLRHCEAWF
AKGAIAEPPPQ

Sequences:

>Translated_571_residues
MTGASFDVPPVLIVGAGPTGLAAAMSLARAHVPVRLIDKAPQADPHSRAIGIQARTLELLEQHRIVDRFLELGHRARTAN
LYSNGHRLARLDFDPLQTRYPYLLFLEQSVTEHLLTEHLATFGVQVERGTELTMFAQGSAGINATLQRLDGHVETLHPSY
MIAADGAHSAIRHRLGMSFAGKTFEQTFLLADLEADTGWSDDEFHIFASGAGLAALFPMGKGRHRLIADHPAMSPSVPST
GDAAPLAAIPAPTLDECRLIAKSRIHHPIDITGLSWSGYFHLNSRMVEQLRAQRVFLAGDAAHVHSPAGAQGMNTGIQEA
FNLGWKIARVLKGDAPERLLDTYHLERHPIERDVLRQTSFVTQLAEADHGPLKLLRERVMPVLAALGPLRDAARLTVSEL
AIQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGTGCIFDLHDPAFFSLFLLVDPPEEAASSNGKLLRLARK
PVRSADLDRFVCAVEGLLPGAVRVWRVSDASGDGGGPSLSESFGRTRPSFYLVRPDGYVCARGRPGSDLNGLLRHCEAWF
AKGAIAEPPPQ
>Mature_570_residues
TGASFDVPPVLIVGAGPTGLAAAMSLARAHVPVRLIDKAPQADPHSRAIGIQARTLELLEQHRIVDRFLELGHRARTANL
YSNGHRLARLDFDPLQTRYPYLLFLEQSVTEHLLTEHLATFGVQVERGTELTMFAQGSAGINATLQRLDGHVETLHPSYM
IAADGAHSAIRHRLGMSFAGKTFEQTFLLADLEADTGWSDDEFHIFASGAGLAALFPMGKGRHRLIADHPAMSPSVPSTG
DAAPLAAIPAPTLDECRLIAKSRIHHPIDITGLSWSGYFHLNSRMVEQLRAQRVFLAGDAAHVHSPAGAQGMNTGIQEAF
NLGWKIARVLKGDAPERLLDTYHLERHPIERDVLRQTSFVTQLAEADHGPLKLLRERVMPVLAALGPLRDAARLTVSELA
IQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGTGCIFDLHDPAFFSLFLLVDPPEEAASSNGKLLRLARKP
VRSADLDRFVCAVEGLLPGAVRVWRVSDASGDGGGPSLSESFGRTRPSFYLVRPDGYVCARGRPGSDLNGLLRHCEAWFA
KGAIAEPPPQ

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=352, Percent_Identity=28.125, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 61957; Mature: 61826

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGASFDVPPVLIVGAGPTGLAAAMSLARAHVPVRLIDKAPQADPHSRAIGIQARTLELL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEECCHHHHHHHH
EQHRIVDRFLELGHRARTANLYSNGHRLARLDFDPLQTRYPYLLFLEQSVTEHLLTEHLA
HHHHHHHHHHHHCCHHEECHHHCCCCEEEEECCCCHHCCCCEEEEEHHHHHHHHHHHHHH
TFGVQVERGTELTMFAQGSAGINATLQRLDGHVETLHPSYMIAADGAHSAIRHRLGMSFA
HHCEEEECCCEEEEEECCCCCHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHCCCCC
GKTFEQTFLLADLEADTGWSDDEFHIFASGAGLAALFPMGKGRHRLIADHPAMSPSVPST
CCHHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCCCCC
GDAAPLAAIPAPTLDECRLIAKSRIHHPIDITGLSWSGYFHLNSRMVEQLRAQRVFLAGD
CCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHCEEEEECC
AAHVHSPAGAQGMNTGIQEAFNLGWKIARVLKGDAPERLLDTYHLERHPIERDVLRQTSF
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VTQLAEADHGPLKLLRERVMPVLAALGPLRDAARLTVSELAIQYRRSPLTLERVLDGGPR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
AGERAPDALVHVVDGPLGRAPGTGCIFDLHDPAFFSLFLLVDPPEEAASSNGKLLRLARK
CCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHCCCCCEEEEHHC
PVRSADLDRFVCAVEGLLPGAVRVWRVSDASGDGGGPSLSESFGRTRPSFYLVRPDGYVC
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCEEEECCCCEEE
ARGRPGSDLNGLLRHCEAWFAKGAIAEPPPQ
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TGASFDVPPVLIVGAGPTGLAAAMSLARAHVPVRLIDKAPQADPHSRAIGIQARTLELL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEECCHHHHHHHH
EQHRIVDRFLELGHRARTANLYSNGHRLARLDFDPLQTRYPYLLFLEQSVTEHLLTEHLA
HHHHHHHHHHHHCCHHEECHHHCCCCEEEEECCCCHHCCCCEEEEEHHHHHHHHHHHHHH
TFGVQVERGTELTMFAQGSAGINATLQRLDGHVETLHPSYMIAADGAHSAIRHRLGMSFA
HHCEEEECCCEEEEEECCCCCHHHHHHHHCCCCEEECCCEEEEECCHHHHHHHHHCCCCC
GKTFEQTFLLADLEADTGWSDDEFHIFASGAGLAALFPMGKGRHRLIADHPAMSPSVPST
CCHHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEEECCCCCCCCCCCC
GDAAPLAAIPAPTLDECRLIAKSRIHHPIDITGLSWSGYFHLNSRMVEQLRAQRVFLAGD
CCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHCEEEEECC
AAHVHSPAGAQGMNTGIQEAFNLGWKIARVLKGDAPERLLDTYHLERHPIERDVLRQTSF
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
VTQLAEADHGPLKLLRERVMPVLAALGPLRDAARLTVSELAIQYRRSPLTLERVLDGGPR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
AGERAPDALVHVVDGPLGRAPGTGCIFDLHDPAFFSLFLLVDPPEEAASSNGKLLRLARK
CCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHCCCCCEEEEHHC
PVRSADLDRFVCAVEGLLPGAVRVWRVSDASGDGGGPSLSESFGRTRPSFYLVRPDGYVC
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCEEEECCCCEEE
ARGRPGSDLNGLLRHCEAWFAKGAIAEPPPQ
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]