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The map label for this gene is citA [H]

Identifier: 186475486

GI number: 186475486

Start: 812590

End: 813891

Strand: Direct

Name: citA [H]

Synonym: Bphy_0721

Alternate gene names: 186475486

Gene position: 812590-813891 (Clockwise)

Preceding gene: 186475483

Following gene: 186475488

Centisome position: 23.36

GC content: 63.67

Gene sequence:

>1302_bases
ATGGCAACTGCATCCCATGCTCAAGCAGCGACGGACGAATCCGAGGTCCGCACCGTCTTCCGCGTCGTCAGCGGAAACTT
CCTCGAAATGTACGACTTCATGGTCTATGGCTACTACGCCGCTGCGATCGCGAAGACGTATTTTCCGGGCGGCAACGAAT
TCGCTTCGCTGATGCTGTCGCTGTCGGTGTTCGGCGCGGGCTTCCTGATGCGTCCGCTCGGCGCCATCGTGCTCGGCGCG
TACATCGACCATCACGGCCGCCGCAAAGGACTGATTCTCACGATCGCGCTGATGGCGCTGGGCACGCTCACGGTCGCGGC
GATTCCCGGCTACGCGACGATCGGCGTGCTCGCGCCCGTGCTCGTGCTGCTTGGGCGCCTGTTGCAGGGCTTCTCGGCGG
GTGTCGAGCTGGGCGGCGTGTCGGTGTATTTGTCGGAGATCGCAACGAGGGGCAACAAGGGCTTCTATTGCTCGTGGCAG
TCGGGCAGTCAGCAGGTGGCCGTAGTGTTCGCCGCGCTGATCGGCGTGTTCCTGAACCGCCTGCTGCCCGCCGACCAGAT
GACGGCATGGGGCTGGCGCGTGCCGTTCCTGATCGGCTGTCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGCTGA
AAGAAACGGAGGAATTCCTCGCGAAGAAGCATCGCCCGTCGATTGGCGAGATTTTCCGTTCGATGGCGCAGAACTGGGGC
GTCGTGCTGGGCGGCATGGGCATGGTGATCATGACGACGGTGTCGTTCTACATGATCACCGCGTACACGCCGACCTTCGG
CAAGGAAGTGCTGAAGCTCTCCGCAATCGACACCCTGATCGTCACCGTGTGCATCGGCGTATCGAACCTGATCTGGCTGC
CCATCTCGGGCGCGATTTCGGACCGGGTCGGTCGCCGTCCCGTGCTGCTGACGTTCACAATCCTGACGATCCTCACGTCG
TACCCGGCCGTGCAGTGGCTCGTTGCCGATCCATCGTTCGCGCGCCTGCTGATGGTCGAGCTGTGGCTGTCGTTCCTGTA
TGGCTGCTACAACGGCGGCATGGTCGTCGCGCTGACGGAAGTGATGCCGGCCGACGTGCGCACGGCAGGCTTCTCGCTCG
CGTATAGCCTCGCGACGACGATCGGCGGCTTCACGCCGGCAATTGCCACGTACCTGATCCATTCGACGGGCAACAAGGCG
GCGCCCGGGCTGTGGATGAGCGTCGCCGCCATCTGCGGTCTGATCGCGACCCTCGTGCTGTATCGCACGGAAGAAGCCCG
CAACCGGTACCGGACGGCCTGA

Upstream 100 bases:

>100_bases
ACCTGGCCGCGCCGGAGCGGGCATCCGCGCTCCCCGCGGATCGTTTGAACTGCCCAACGCTGCTTGCGATGCAGATAACG
GGCCCATTCCGAGAAAGTCA

Downstream 100 bases:

>100_bases
GCCGTCGCGTGTTATGGGGCGCGTGCCCCGACGCTGTAGAACCAGACCCCCGCACGTGTGCGGGGGTTTTCATTTCATCG
GCCGTTTCGGCGATGCAGTA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MATASHAQAATDESEVRTVFRVVSGNFLEMYDFMVYGYYAAAIAKTYFPGGNEFASLMLSLSVFGAGFLMRPLGAIVLGA
YIDHHGRRKGLILTIALMALGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATRGNKGFYCSWQ
SGSQQVAVVFAALIGVFLNRLLPADQMTAWGWRVPFLIGCLIVPFLFLIRRSLKETEEFLAKKHRPSIGEIFRSMAQNWG
VVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSAIDTLIVTVCIGVSNLIWLPISGAISDRVGRRPVLLTFTILTILTS
YPAVQWLVADPSFARLLMVELWLSFLYGCYNGGMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAIATYLIHSTGNKA
APGLWMSVAAICGLIATLVLYRTEEARNRYRTA

Sequences:

>Translated_433_residues
MATASHAQAATDESEVRTVFRVVSGNFLEMYDFMVYGYYAAAIAKTYFPGGNEFASLMLSLSVFGAGFLMRPLGAIVLGA
YIDHHGRRKGLILTIALMALGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATRGNKGFYCSWQ
SGSQQVAVVFAALIGVFLNRLLPADQMTAWGWRVPFLIGCLIVPFLFLIRRSLKETEEFLAKKHRPSIGEIFRSMAQNWG
VVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSAIDTLIVTVCIGVSNLIWLPISGAISDRVGRRPVLLTFTILTILTS
YPAVQWLVADPSFARLLMVELWLSFLYGCYNGGMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAIATYLIHSTGNKA
APGLWMSVAAICGLIATLVLYRTEEARNRYRTA
>Mature_432_residues
ATASHAQAATDESEVRTVFRVVSGNFLEMYDFMVYGYYAAAIAKTYFPGGNEFASLMLSLSVFGAGFLMRPLGAIVLGAY
IDHHGRRKGLILTIALMALGTLTVAAIPGYATIGVLAPVLVLLGRLLQGFSAGVELGGVSVYLSEIATRGNKGFYCSWQS
GSQQVAVVFAALIGVFLNRLLPADQMTAWGWRVPFLIGCLIVPFLFLIRRSLKETEEFLAKKHRPSIGEIFRSMAQNWGV
VLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSAIDTLIVTVCIGVSNLIWLPISGAISDRVGRRPVLLTFTILTILTSY
PAVQWLVADPSFARLLMVELWLSFLYGCYNGGMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAIATYLIHSTGNKAA
PGLWMSVAAICGLIATLVLYRTEEARNRYRTA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=30.715935334873, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=32.7906976744186, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788292, Length=425, Percent_Identity=26.3529411764706, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=28.9954337899543, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87082231, Length=168, Percent_Identity=30.3571428571429, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46837; Mature: 46706

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCC
>Mature Secondary Structure 
ATASHAQAATDESEVRTVFRVVSGNFLEMYDFMVYGYYAAAIAKTYFPGGNEFASLMLS
CCCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH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HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]