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The map label for this gene is rph

Identifier: 186475433

GI number: 186475433

Start: 752764

End: 753504

Strand: Reverse

Name: rph

Synonym: Bphy_0667

Alternate gene names: 186475433

Gene position: 753504-752764 (Counterclockwise)

Preceding gene: 186475440

Following gene: 186475432

Centisome position: 21.66

GC content: 65.99

Gene sequence:

>741_bases
ATGAACGACATCACGAAACGCCCCAGCGGCCGCACCGCCGATCAGCTGCGCGACGTGCGCATCACGCGCCATTACACGAA
GCACGCGGAAGGCTCCGTGCTGGTCGAATTCGGCGATACCAAGGTGATCTGCACGGCGAGCATCGCCGAGAGCGTGCCCG
CCTTTCTGCGCGACCGCGGCCAGGGCTGGCTGACGGCCGAATACGGCATGCTGCCGCGCGCGACGCACACACGCAGCGAC
CGCGAAGCCGCACGCGGCAAGCAGACCGGGCGGACGCAGGAAATCCAGCGCCTGATCGGCCGCGCGCTGCGCTCGGTGTT
CGATCTCGAGCGGCTCGGCGCGCGCACGCTGCATATCGACTGCGACGTGATCCAGGCCGACGGCGGCACGCGCACGGCGA
GCATCACGGGCGCTTTCGTCGCCGCACGCGACGCCGTCGCGAAGCTGCTCGCGACGGGTCGCATCGAGACGTCGCCCATC
ACCGACTACGTCGCAGCCATCTCGGTCGGCGTGTTTGACGGCATCCCCGTGCTCGATCTCGACTATGACGAAGACTCGCA
ATGCGACACCGACATGAATGTCGTGATGACGGGCGACGGCGGCTTTGTCGAAATCCAGGGCACGGCAGAAGGCGTGCCCT
TCTCGCGCGATGAAATGAACAAGCTGCTCGATCTCGCGCAAGCCGGCATTGCGACGCTGATCGCGAAGCAGAAGGAAGCA
TTGGAGTCCAAGGGTGAGTGA

Upstream 100 bases:

>100_bases
TCGCGCCGCGCTGCATCGCGTTGCGCGGTGCGGGCGGCGCGAGCGCGATCGGCGGTAAAATTGCAGTTTCCTTCCGCCCT
ATCTTCCCCCTACTCCGACG

Downstream 100 bases:

>100_bases
TCGTCAGGACAACGCGCTGCGCAAGATCGTGCTGGCGTCGAACAACGCGGGCAAGCTGCGCGAGTTCGCCGCGCTGTTCG
GTACGGCGGGCATCGAGCTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNDITKRPSGRTADQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPAFLRDRGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRSVFDLERLGARTLHIDCDVIQADGGTRTASITGAFVAARDAVAKLLATGRIETSPI
TDYVAAISVGVFDGIPVLDLDYDEDSQCDTDMNVVMTGDGGFVEIQGTAEGVPFSRDEMNKLLDLAQAGIATLIAKQKEA
LESKGE

Sequences:

>Translated_246_residues
MNDITKRPSGRTADQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPAFLRDRGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRSVFDLERLGARTLHIDCDVIQADGGTRTASITGAFVAARDAVAKLLATGRIETSPI
TDYVAAISVGVFDGIPVLDLDYDEDSQCDTDMNVVMTGDGGFVEIQGTAEGVPFSRDEMNKLLDLAQAGIATLIAKQKEA
LESKGE
>Mature_246_residues
MNDITKRPSGRTADQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPAFLRDRGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRSVFDLERLGARTLHIDCDVIQADGGTRTASITGAFVAARDAVAKLLATGRIETSPI
TDYVAAISVGVFDGIPVLDLDYDEDSQCDTDMNVVMTGDGGFVEIQGTAEGVPFSRDEMNKLLDLAQAGIATLIAKQKEA
LESKGE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=66.5071770334928, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71981632, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_BURP8 (B2JEI0)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856903.1
- ProteinModelPortal:   B2JEI0
- SMR:   B2JEI0
- GeneID:   6242166
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0667
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26656; Mature: 26656

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDITKRPSGRTADQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPAFLRDRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHCC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRSVFDLERLGARTLHID
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
CDVIQADGGTRTASITGAFVAARDAVAKLLATGRIETSPITDYVAAISVGVFDGIPVLDL
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEC
DYDEDSQCDTDMNVVMTGDGGFVEIQGTAEGVPFSRDEMNKLLDLAQAGIATLIAKQKEA
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LESKGE
HHCCCC
>Mature Secondary Structure
MNDITKRPSGRTADQLRDVRITRHYTKHAEGSVLVEFGDTKVICTASIAESVPAFLRDRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHCC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRSVFDLERLGARTLHID
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
CDVIQADGGTRTASITGAFVAARDAVAKLLATGRIETSPITDYVAAISVGVFDGIPVLDL
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEC
DYDEDSQCDTDMNVVMTGDGGFVEIQGTAEGVPFSRDEMNKLLDLAQAGIATLIAKQKEA
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LESKGE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA