Click here to switch to the map view.

The map label for this gene is bah [H]

Identifier: 186475129

GI number: 186475129

Start: 407857

End: 408807

Strand: Direct

Name: bah [H]

Synonym: Bphy_0360

Alternate gene names: 186475129

Gene position: 407857-408807 (Clockwise)

Preceding gene: 186475128

Following gene: 186475131

Centisome position: 11.72

GC content: 67.61

Gene sequence:

>951_bases
ATGAGTTGGCAAAGCGCTCTTGCGTGCTGGTTCCTGCGCCGCCAGTTTCATCCGCAGACGCGGCACCCGATCATCGACGC
GCGGCGCGCACGGAGGATGACCGCGAAGCGCGCCTACACGCCGCGCGTGCCGTCGGGCTGGACCTTGCACGAACGATATG
GCGCACATGATGCGCCGCTGCGCGGCGAATGGCTGACCCGCGTCGATGGCGTGCACAAAGCCACGATTCTCTACTTGCAT
GGCGGCGGCTACTACTTCTGCTCGCCGCAGACGCATCGCGCGGCGTCGTTCGGCCTTGCCGCGCGCAGTGGGGCGCGCGT
CTTTTCGCTCGATTACCGCCTTGCTCCCGAACAGCCGTTCCCCGCCGCGCTCGATGACGCGCTTGCCGCGTATCGCCGGT
TGATGGAAGAAGGGTCGAAGGCAGAGTCGATTGTGATCGCGGGTGATTCGGCGGGCGGCGGCCTGGCGCTCGCGACGCTC
GTCGCATTGCGCGACGCAGGCGAGCCGATGCCGGCTGGCGCGATCCTGTTTTCGCCGTGGACCGATCTCGCGGCGACGGG
CGCGTCGCTGCAAACCAACGACGGTGCCGATCCGATGTTCCACGGTCCGTCCATCGCGCGCGCCGCGCATCTTTATCTTG
GCGGCACGCCCGCGACGCATCCGTACGCTTCGCCGCTCTACGCGGACCTGAAAGGCCTGCCGCCGCTCTTCATGCAGGTG
AGCAGCACGGAAGTCCTGCTCGACGACTCACGTCGGGTCGCCGACAAGGCGCGGGCGGCCGGCGTCGAAATCGACTTCGA
GATCTGGCCCAATATGCCGCACGTGTGGCAGCTCTGGGTGCCGTTCATTCCCGAGGCGGGCCGCGCGCTCGACCTCGCCG
CGCGTTTCGTCGTGCGCGTGACGGACGAGTCGCGCAACGTTCAGCCGTCGAGCGAAACCTCGATCGCCTGA

Upstream 100 bases:

>100_bases
CGAAGACCAGTCATGCGCGCGTTCAGTAGAAGCCGCCGCGGACTCGTCCGGGCCAAATCCGCGTACCATGAGCGCTTGAC
CGTCGATGGAGAATGCTGCG

Downstream 100 bases:

>100_bases
TACGCGGCCTGCACGACATCTGTGCCGTACTGGCGTTCGAGCCGTCTCACAGTGAAATGCCCGTGTGCGACCTGCTGGAA
GTGATCGATGAACACGGTGT

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSWQSALACWFLRRQFHPQTRHPIIDARRARRMTAKRAYTPRVPSGWTLHERYGAHDAPLRGEWLTRVDGVHKATILYLH
GGGYYFCSPQTHRAASFGLAARSGARVFSLDYRLAPEQPFPAALDDALAAYRRLMEEGSKAESIVIAGDSAGGGLALATL
VALRDAGEPMPAGAILFSPWTDLAATGASLQTNDGADPMFHGPSIARAAHLYLGGTPATHPYASPLYADLKGLPPLFMQV
SSTEVLLDDSRRVADKARAAGVEIDFEIWPNMPHVWQLWVPFIPEAGRALDLAARFVVRVTDESRNVQPSSETSIA

Sequences:

>Translated_316_residues
MSWQSALACWFLRRQFHPQTRHPIIDARRARRMTAKRAYTPRVPSGWTLHERYGAHDAPLRGEWLTRVDGVHKATILYLH
GGGYYFCSPQTHRAASFGLAARSGARVFSLDYRLAPEQPFPAALDDALAAYRRLMEEGSKAESIVIAGDSAGGGLALATL
VALRDAGEPMPAGAILFSPWTDLAATGASLQTNDGADPMFHGPSIARAAHLYLGGTPATHPYASPLYADLKGLPPLFMQV
SSTEVLLDDSRRVADKARAAGVEIDFEIWPNMPHVWQLWVPFIPEAGRALDLAARFVVRVTDESRNVQPSSETSIA
>Mature_315_residues
SWQSALACWFLRRQFHPQTRHPIIDARRARRMTAKRAYTPRVPSGWTLHERYGAHDAPLRGEWLTRVDGVHKATILYLHG
GGYYFCSPQTHRAASFGLAARSGARVFSLDYRLAPEQPFPAALDDALAAYRRLMEEGSKAESIVIAGDSAGGGLALATLV
ALRDAGEPMPAGAILFSPWTDLAATGASLQTNDGADPMFHGPSIARAAHLYLGGTPATHPYASPLYADLKGLPPLFMQVS
STEVLLDDSRRVADKARAAGVEIDFEIWPNMPHVWQLWVPFIPEAGRALDLAARFVVRVTDESRNVQPSSETSIA

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=185, Percent_Identity=31.8918918918919, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786682, Length=317, Percent_Identity=26.4984227129338, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=266, Percent_Identity=25.187969924812, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34540; Mature: 34408

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQSALACWFLRRQFHPQTRHPIIDARRARRMTAKRAYTPRVPSGWTLHERYGAHDAPL
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCC
RGEWLTRVDGVHKATILYLHGGGYYFCSPQTHRAASFGLAARSGARVFSLDYRLAPEQPF
CCCHHHHHCCCEEEEEEEEECCEEEEECCCCCCHHHHCHHHCCCCEEEEEEEEECCCCCC
PAALDDALAAYRRLMEEGSKAESIVIAGDSAGGGLALATLVALRDAGEPMPAGAILFSPW
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
TDLAATGASLQTNDGADPMFHGPSIARAAHLYLGGTPATHPYASPLYADLKGLPPLFMQV
HHHHHCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCHHHHHCCCCHHHEEE
SSTEVLLDDSRRVADKARAAGVEIDFEIWPNMPHVWQLWVPFIPEAGRALDLAARFVVRV
CCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHHHHEEEEEEE
TDESRNVQPSSETSIA
ECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SWQSALACWFLRRQFHPQTRHPIIDARRARRMTAKRAYTPRVPSGWTLHERYGAHDAPL
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCC
RGEWLTRVDGVHKATILYLHGGGYYFCSPQTHRAASFGLAARSGARVFSLDYRLAPEQPF
CCCHHHHHCCCEEEEEEEEECCEEEEECCCCCCHHHHCHHHCCCCEEEEEEEEECCCCCC
PAALDDALAAYRRLMEEGSKAESIVIAGDSAGGGLALATLVALRDAGEPMPAGAILFSPW
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCC
TDLAATGASLQTNDGADPMFHGPSIARAAHLYLGGTPATHPYASPLYADLKGLPPLFMQV
HHHHHCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCHHHHHCCCCHHHEEE
SSTEVLLDDSRRVADKARAAGVEIDFEIWPNMPHVWQLWVPFIPEAGRALDLAARFVVRV
CCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCCHHHHHEEEEEEE
TDESRNVQPSSETSIA
ECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]