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The map label for this gene is rhtB [H]

Identifier: 186475103

GI number: 186475103

Start: 375702

End: 376316

Strand: Direct

Name: rhtB [H]

Synonym: Bphy_0334

Alternate gene names: 186475103

Gene position: 375702-376316 (Clockwise)

Preceding gene: 186475102

Following gene: 186475104

Centisome position: 10.8

GC content: 63.41

Gene sequence:

>615_bases
ATGCCCAACTTCCTGCTTTTTCTCGCGACGTCGATAGCGATCACGTTTGCGCCCGGTCCCGACAATCTGCAGGTTCTCGC
GCGCGGCATTTCGCAGGGACGCGCGGCGGGCTTCGTCGCGGCGCTCGGCTTCGCGGCGGGCATTTCATTTCACACCACAC
TTGCCGCGCTCGGCATCGCGGCCGTGCTGCGCTCGTCGCCCATCGCGTTCGAAGTGCTCAAGCTCGCGGGTGCCGCGTAC
CTGATCTGGATCGGCATCAAGGCATTGCGCAGCAAAGGTCTCGCAACCGCGCACGAACGCGCGCCGCAGCCGCTTTCATC
GATCTTCCGGCAAAGCGTGATCGGCAACATGCTGAACCCGAAAGTCACGTTGTTCTTCGTCGTGTTCCTGCCGCAATTCG
TCGACCCGCATGGCGCACAAAGCGTCACGCTGCAGATGCTCGAACTGGGCGCGCTGTTCATGCTGCAGACGGTTATCGTG
TTTTCGCTGTTCGGGGTGTGTGCGGGCATGATCGGCGGATGGCTGAAGCGCCGTCCGCGTGCGGGCGTGTGGCTCGATCG
TCTCGCGGGTGCGACCTTCATCGCAATCGGCATTCGCGTCGCGTTGCGCGACTGA

Upstream 100 bases:

>100_bases
GACCTCGGCGGCTCGAAGTTGCTGCGCGGCGCGGGCCTTCCGCTTTTCACCGTCACGTCGTTCGGCGGCCACTGATCTCA
AACAACGGAGTGTTTGCGCG

Downstream 100 bases:

>100_bases
GTCGGGTCGGTACTCTTGCTCGCGCTTTATTCATCACGCAGTCCGGAGTCTTCAATGTCCTTGCCTTGCATCGTCGCCGC
GCGCCGTTTCGATCGTGCGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPNFLLFLATSIAITFAPGPDNLQVLARGISQGRAAGFVAALGFAAGISFHTTLAALGIAAVLRSSPIAFEVLKLAGAAY
LIWIGIKALRSKGLATAHERAPQPLSSIFRQSVIGNMLNPKVTLFFVVFLPQFVDPHGAQSVTLQMLELGALFMLQTVIV
FSLFGVCAGMIGGWLKRRPRAGVWLDRLAGATFIAIGIRVALRD

Sequences:

>Translated_204_residues
MPNFLLFLATSIAITFAPGPDNLQVLARGISQGRAAGFVAALGFAAGISFHTTLAALGIAAVLRSSPIAFEVLKLAGAAY
LIWIGIKALRSKGLATAHERAPQPLSSIFRQSVIGNMLNPKVTLFFVVFLPQFVDPHGAQSVTLQMLELGALFMLQTVIV
FSLFGVCAGMIGGWLKRRPRAGVWLDRLAGATFIAIGIRVALRD
>Mature_203_residues
PNFLLFLATSIAITFAPGPDNLQVLARGISQGRAAGFVAALGFAAGISFHTTLAALGIAAVLRSSPIAFEVLKLAGAAYL
IWIGIKALRSKGLATAHERAPQPLSSIFRQSVIGNMLNPKVTLFFVVFLPQFVDPHGAQSVTLQMLELGALFMLQTVIVF
SLFGVCAGMIGGWLKRRPRAGVWLDRLAGATFIAIGIRVALRD

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=31.25, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788099, Length=204, Percent_Identity=28.4313725490196, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786522, Length=115, Percent_Identity=28.695652173913, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21688; Mature: 21557

Theoretical pI: Translated: 11.90; Mature: 11.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFLLFLATSIAITFAPGPDNLQVLARGISQGRAAGFVAALGFAAGISFHTTLAALGIA
CCCHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRSSPIAFEVLKLAGAAYLIWIGIKALRSKGLATAHERAPQPLSSIFRQSVIGNMLNP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVDPHGAQSVTLQMLELGALFMLQTVIVFSLFGVCAGMIGGWLKRRPR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGVWLDRLAGATFIAIGIRVALRD
CCHHHHHHCCHHHHHHHHHHEECC
>Mature Secondary Structure 
PNFLLFLATSIAITFAPGPDNLQVLARGISQGRAAGFVAALGFAAGISFHTTLAALGIA
CCHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLRSSPIAFEVLKLAGAAYLIWIGIKALRSKGLATAHERAPQPLSSIFRQSVIGNMLNP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCC
KVTLFFVVFLPQFVDPHGAQSVTLQMLELGALFMLQTVIVFSLFGVCAGMIGGWLKRRPR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGVWLDRLAGATFIAIGIRVALRD
CCHHHHHHCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]