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The map label for this gene is mutY [H]

Identifier: 186475089

GI number: 186475089

Start: 362176

End: 363285

Strand: Direct

Name: mutY [H]

Synonym: Bphy_0320

Alternate gene names: 186475089

Gene position: 362176-363285 (Clockwise)

Preceding gene: 186475088

Following gene: 186475101

Centisome position: 10.41

GC content: 65.5

Gene sequence:

>1110_bases
ATGTCCGTTTCCGCTCCCGTTTCGTCCGCCCCTCGTTCGACGATACCCGCACTATCCGATTTCTCTGTGCGCCTGATCGC
ATGGCAGCGCGAACACGGCCGTCACGATCTGCCATGGCAGAACACGCGCGATCCATACCGTATCTGGCTGTCGGAAATCA
TGTTGCAGCAGACGCAGGTGTCGACGGTGATCCCGTACTACGCGCGTTTTCTCGCGCGTTTTCCGGATGTCGCCGCGCTC
GCGTCGGCGCCCGCGGATGACGTGATGACCTTGTGGGCCGGTCTCGGCTACTACACGCGCGCGCGAAATCTGCATCGCTG
CGCGCAGGTCGTCGTCGAGCAGCATGGCGGCCGTTTTCCCGAATCCGTCGACGCGCTGGCGGAGTTGCCGGGCATCGGAC
GCTCGACGGCTGCCGCGATTGCGTCATTCGCGTTCGGCGCACGCGCGACGATTCTCGACGGCAACGTGAAGCGCGTGCTC
GCGCGAGTGTTCGGCGTGGAAGGTTATCCGGGCGAGAAGAAAGTCGAGAACGGCATGTGGCTGCTGGCGGAATCGTTGCT
GCCCGTCAACGCGACGGACGACGACATCAGCGCCTACACGCAGGGCCTGATGGACTTCGGCGCGACGCTGTGTGCGCGCG
GCAAGCCGGATTGCGTGCGCTGTCCGTTCGCGGTCGATTGCGTGGCTCACGTGACGGGGCGTCAGCGCGAATTGCCAGCC
GCGCGCCCGAAAAAGACGGTGCCGACGCGACGCACGTGGATGCTCCTGTTGCGCGATGGCGATTCGGTGATGCTGGAAAA
GCGCCCGCCGTCGGGCATCTGGGGCGGCTTGTGGAGTCTTCCGGAAGCGGCGGGTGAAGATGCGCTCTCGCAACTCGCGC
GCGACTTGGGCGCGCGCGCCGCTGTATCGCCGCTCGCGCCGCTCACGCATGTATTCACGCACTTCAGGCTCGAGATCGAA
CCGCGCATCGCTGAGTTCGATCATGCGTCGAAGACCGTGAGCGCGCGCGATGCCGACACCGCGTGGATGTCGCTGAACGA
ACTCGACGCGTACGGCGTGCCGGCGCCCGTGCGCAAGCTGCTGGAAGCACTGCAGGGCTCGCTGATCTGA

Upstream 100 bases:

>100_bases
GCGGGTCAGCCATGCCGCGTGTGCGGCACGCCGATCAAACAGATCGTGCAGGGCCAACGCTCCACTTACTACTGTCCGAC
CTGTCAGCGCTGAGCCGTTC

Downstream 100 bases:

>100_bases
AGCGCTGCCGTTACAGCAGTTGATGCTGCTGCATGTAGTGATGCACGACGTCGATGCGCGCGCCGGCGTCTTGTAGTTCC
ATCAGCTTTTGCCGCGCTTT

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSVSAPVSSAPRSTIPALSDFSVRLIAWQREHGRHDLPWQNTRDPYRIWLSEIMLQQTQVSTVIPYYARFLARFPDVAAL
ASAPADDVMTLWAGLGYYTRARNLHRCAQVVVEQHGGRFPESVDALAELPGIGRSTAAAIASFAFGARATILDGNVKRVL
ARVFGVEGYPGEKKVENGMWLLAESLLPVNATDDDISAYTQGLMDFGATLCARGKPDCVRCPFAVDCVAHVTGRQRELPA
ARPKKTVPTRRTWMLLLRDGDSVMLEKRPPSGIWGGLWSLPEAAGEDALSQLARDLGARAAVSPLAPLTHVFTHFRLEIE
PRIAEFDHASKTVSARDADTAWMSLNELDAYGVPAPVRKLLEALQGSLI

Sequences:

>Translated_369_residues
MSVSAPVSSAPRSTIPALSDFSVRLIAWQREHGRHDLPWQNTRDPYRIWLSEIMLQQTQVSTVIPYYARFLARFPDVAAL
ASAPADDVMTLWAGLGYYTRARNLHRCAQVVVEQHGGRFPESVDALAELPGIGRSTAAAIASFAFGARATILDGNVKRVL
ARVFGVEGYPGEKKVENGMWLLAESLLPVNATDDDISAYTQGLMDFGATLCARGKPDCVRCPFAVDCVAHVTGRQRELPA
ARPKKTVPTRRTWMLLLRDGDSVMLEKRPPSGIWGGLWSLPEAAGEDALSQLARDLGARAAVSPLAPLTHVFTHFRLEIE
PRIAEFDHASKTVSARDADTAWMSLNELDAYGVPAPVRKLLEALQGSLI
>Mature_368_residues
SVSAPVSSAPRSTIPALSDFSVRLIAWQREHGRHDLPWQNTRDPYRIWLSEIMLQQTQVSTVIPYYARFLARFPDVAALA
SAPADDVMTLWAGLGYYTRARNLHRCAQVVVEQHGGRFPESVDALAELPGIGRSTAAAIASFAFGARATILDGNVKRVLA
RVFGVEGYPGEKKVENGMWLLAESLLPVNATDDDISAYTQGLMDFGATLCARGKPDCVRCPFAVDCVAHVTGRQRELPAA
RPKKTVPTRRTWMLLLRDGDSVMLEKRPPSGIWGGLWSLPEAAGEDALSQLARDLGARAAVSPLAPLTHVFTHFRLEIEP
RIAEFDHASKTVSARDADTAWMSLNELDAYGVPAPVRKLLEALQGSLI

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=417, Percent_Identity=30.6954436450839, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI190358497, Length=417, Percent_Identity=30.6954436450839, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI6912520, Length=417, Percent_Identity=30.6954436450839, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI115298650, Length=417, Percent_Identity=30.6954436450839, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI115298654, Length=417, Percent_Identity=30.6954436450839, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI115298652, Length=417, Percent_Identity=30.6954436450839, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789331, Length=348, Percent_Identity=46.551724137931, Blast_Score=333, Evalue=8e-93,
Organism=Escherichia coli, GI1787920, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 40382; Mature: 40250

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSAPVSSAPRSTIPALSDFSVRLIAWQREHGRHDLPWQNTRDPYRIWLSEIMLQQTQV
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
STVIPYYARFLARFPDVAALASAPADDVMTLWAGLGYYTRARNLHRCAQVVVEQHGGRFP
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESVDALAELPGIGRSTAAAIASFAFGARATILDGNVKRVLARVFGVEGYPGEKKVENGMW
HHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHCCHH
LLAESLLPVNATDDDISAYTQGLMDFGATLCARGKPDCVRCPFAVDCVAHVTGRQRELPA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCCCCC
ARPKKTVPTRRTWMLLLRDGDSVMLEKRPPSGIWGGLWSLPEAAGEDALSQLARDLGARA
CCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCHHHHCCHHCCHHHHHHHHHHHCCHH
AVSPLAPLTHVFTHFRLEIEPRIAEFDHASKTVSARDADTAWMSLNELDAYGVPAPVRKL
HHCHHHHHHHHHHHHEEEECCCHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LEALQGSLI
HHHHHCCCC
>Mature Secondary Structure 
SVSAPVSSAPRSTIPALSDFSVRLIAWQREHGRHDLPWQNTRDPYRIWLSEIMLQQTQV
CCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
STVIPYYARFLARFPDVAALASAPADDVMTLWAGLGYYTRARNLHRCAQVVVEQHGGRFP
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ESVDALAELPGIGRSTAAAIASFAFGARATILDGNVKRVLARVFGVEGYPGEKKVENGMW
HHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCCCHHHHCCHH
LLAESLLPVNATDDDISAYTQGLMDFGATLCARGKPDCVRCPFAVDCVAHVTGRQRELPA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHCCCCCCCCC
ARPKKTVPTRRTWMLLLRDGDSVMLEKRPPSGIWGGLWSLPEAAGEDALSQLARDLGARA
CCCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCHHHHCCHHCCHHHHHHHHHHHCCHH
AVSPLAPLTHVFTHFRLEIEPRIAEFDHASKTVSARDADTAWMSLNELDAYGVPAPVRKL
HHCHHHHHHHHHHHHEEEECCCHHHHCCHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LEALQGSLI
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]