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The map label for this gene is mutM

Identifier: 186475088

GI number: 186475088

Start: 361338

End: 362168

Strand: Direct

Name: mutM

Synonym: Bphy_0319

Alternate gene names: 186475088

Gene position: 361338-362168 (Clockwise)

Preceding gene: 186475080

Following gene: 186475089

Centisome position: 10.39

GC content: 62.94

Gene sequence:

>831_bases
ATGCCAGAGTTGCCAGAAGTTGAGGTTACCCGACGAGGAATCGAACCTTACGTCGCGGGCCGCCGCGTCGAACGCGTGGA
CGTGCGCACGCCCGCGTTGCGCTGGCCGATTCCGCCGGGGTTCGCGCGTCTTCTGCACGGCCGGCTGGTCCGAAAGGTGG
AACGGCGCGGCAAATATCTGCTCTTTGAAATCGACGAAGGCTGGTTCATCGTTCATCTCGGCATGACAGGAACGCTCAGG
GTCCTGCGCAACGTACCGCATCCGCCTGCAGCGGCGAAGCATGATCACGTTGACTGGATCTTCGATGACTTCATTTTGCG
CTTCCGCGACCCGCGACGCTTCGGCGCTGTGTTATGGCACCCGCGCTCGGCAGGCGATGTTCTCGATCATCCGCTGCTGG
CCGACCTGGGTGTCGAGCCGTTCGCACCGTCGTTTTCAGGCGCGCTGCTGCATCGGAAGACACGCGGACGCAAGGTGTCG
GTGAAGCAGGCGTTGCTTGCGGGCGAGATCGTCGTCGGTGTGGGCAACATTTACGCTTCTGAAAGCCTGTTTCGTGCCGG
CATCAGGCCGACCACGCCCGCCGGTCGGATCTCGCTCGTTCGGTACGATCTGCTTGCAGACGCCGTGCGCGTCACGCTGG
CCGCCGCGATCGAAAAGGGCGGCAGCACGTTGCGCGACTTCGTGGGCAGCAACGGCGAAAGCGGCTACTTCCAGCTCGAC
TATTTCGTCTATGATCGCGCGGGTCAGCCATGCCGCGTGTGCGGCACGCCGATCAAACAGATCGTGCAGGGCCAACGCTC
CACTTACTACTGTCCGACCTGTCAGCGCTGA

Upstream 100 bases:

>100_bases
TGGACAGGTTCATACAAATCCGTTCAATGTTTCGGTCGATTGTAACGCGCTCGCTACAATAAGCGCATAACCACTCACGC
AAGTTGCAGACCAGTTAGAC

Downstream 100 bases:

>100_bases
GCCGTTCATGTCCGTTTCCGCTCCCGTTTCGTCCGCCCCTCGTTCGACGATACCCGCACTATCCGATTTCTCTGTGCGCC
TGATCGCATGGCAGCGCGAA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MPELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVRKVERRGKYLLFEIDEGWFIVHLGMTGTLR
VLRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWHPRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVS
VKQALLAGEIVVGVGNIYASESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLD
YFVYDRAGQPCRVCGTPIKQIVQGQRSTYYCPTCQR

Sequences:

>Translated_276_residues
MPELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVRKVERRGKYLLFEIDEGWFIVHLGMTGTLR
VLRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWHPRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVS
VKQALLAGEIVVGVGNIYASESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLD
YFVYDRAGQPCRVCGTPIKQIVQGQRSTYYCPTCQR
>Mature_275_residues
PELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVRKVERRGKYLLFEIDEGWFIVHLGMTGTLRV
LRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWHPRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVSV
KQALLAGEIVVGVGNIYASESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLDY
FVYDRAGQPCRVCGTPIKQIVQGQRSTYYCPTCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=277, Percent_Identity=48.7364620938628, Blast_Score=261, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_BURP8 (B2JCP3)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856558.1
- ProteinModelPortal:   B2JCP3
- SMR:   B2JCP3
- GeneID:   6241821
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0319
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 30948; Mature: 30817

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 266-266 BINDING 94-94 BINDING 112-112 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVRKVERRGKYL
CCCCCCCHHHHCCCCHHHCCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEE
LFEIDEGWFIVHLGMTGTLRVLRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWH
EEEECCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEC
PRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVSVKQALLAGEIVVGVGNIYAS
CCCCCHHHCCCHHHHCCCCCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCEEEEECCHHHH
ESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLD
HHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEE
YFVYDRAGQPCRVCGTPIKQIVQGQRSTYYCPTCQR
EEEEECCCCCHHHCCCHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
PELPEVEVTRRGIEPYVAGRRVERVDVRTPALRWPIPPGFARLLHGRLVRKVERRGKYL
CCCCCCHHHHCCCCHHHCCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEE
LFEIDEGWFIVHLGMTGTLRVLRNVPHPPAAAKHDHVDWIFDDFILRFRDPRRFGAVLWH
EEEECCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCEEEEC
PRSAGDVLDHPLLADLGVEPFAPSFSGALLHRKTRGRKVSVKQALLAGEIVVGVGNIYAS
CCCCCHHHCCCHHHHCCCCCCCCCCCCHHHHHHCCCCEEHHHHHHHHCCEEEEECCHHHH
ESLFRAGIRPTTPAGRISLVRYDLLADAVRVTLAAAIEKGGSTLRDFVGSNGESGYFQLD
HHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEE
YFVYDRAGQPCRVCGTPIKQIVQGQRSTYYCPTCQR
EEEEECCCCCHHHCCCHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA