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The map label for this gene is maiA [H]

Identifier: 186475076

GI number: 186475076

Start: 350142

End: 350786

Strand: Direct

Name: maiA [H]

Synonym: Bphy_0307

Alternate gene names: 186475076

Gene position: 350142-350786 (Clockwise)

Preceding gene: 186475075

Following gene: 186475078

Centisome position: 10.06

GC content: 59.38

Gene sequence:

>645_bases
ATGAAGCTATACAGCTACTTTCGCAGCTCGGCGTCCTATCGCGTGCGCATCGCGCTGAATCTGAAGAATCTGCCGTACGA
GTACGTGCCCGTGCATCTGCTGCGCGACGGCGGCGAGCAGTTCAAGCCCGAATACCGCAAGCTCAATCACGACGCCATCG
TGCCAACGCTGGTGGACAACGGTCATGTGATCACGCAGTCGCTTGCGATCATCGAGTATCTGGAGGAAACGCATCCCGAG
CCGGCGCTGCTGCCGTCGAAGCCTGCCGATCGCGCGTATGTCCGCTCGATCGTCCAGCAGCTTGCGTGCGAAATCCATCC
ATTGAACAATCTGCGCGTGCTCAAGTATTTGAAGCGCACGGTCGGCGTGAGCGACGAGGTGAAGGACGCGTGGTATCACC
ATTGGATCAGTTCGGGTTTTGCTGCGCTCGAAGAGTATCTGGTCGCCGATGGCCGCGCGGGTATGCTGTGCTTCGGCGAT
ACGCCGACCATCGCCGATATCTGCCTCGTGCCGCAGGTGTTCAATGCCTCGCGCTTTAACGTCGATTTGAGCCCCTATCC
GACAATCCGGCGCATTTGCGATTACGCAAACTCGCTGGATGCGTTCGCGCGCGCCGAACCTTCCGTGCAACCGGACGCGG
AGTAA

Upstream 100 bases:

>100_bases
GTCCGTGGCGACCTGATGAAGGGCGGCGTTGAGGGGCTGGGCGAATTCAGCGTGCGCATGGTGTAACGTCCGCCGATTCA
ATCAGGGGGGAGACTACACA

Downstream 100 bases:

>100_bases
ATGCGGGAGCGTTTCGCGACGACTCGTCCGCAACGCGCAAGCAATAAAAAAGGGCGTCAGGCGACCGATGACGCTCTTTT
CGTATTTCGCCTCGCCTTCA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSYFRSSASYRVRIALNLKNLPYEYVPVHLLRDGGEQFKPEYRKLNHDAIVPTLVDNGHVITQSLAIIEYLEETHPE
PALLPSKPADRAYVRSIVQQLACEIHPLNNLRVLKYLKRTVGVSDEVKDAWYHHWISSGFAALEEYLVADGRAGMLCFGD
TPTIADICLVPQVFNASRFNVDLSPYPTIRRICDYANSLDAFARAEPSVQPDAE

Sequences:

>Translated_214_residues
MKLYSYFRSSASYRVRIALNLKNLPYEYVPVHLLRDGGEQFKPEYRKLNHDAIVPTLVDNGHVITQSLAIIEYLEETHPE
PALLPSKPADRAYVRSIVQQLACEIHPLNNLRVLKYLKRTVGVSDEVKDAWYHHWISSGFAALEEYLVADGRAGMLCFGD
TPTIADICLVPQVFNASRFNVDLSPYPTIRRICDYANSLDAFARAEPSVQPDAE
>Mature_214_residues
MKLYSYFRSSASYRVRIALNLKNLPYEYVPVHLLRDGGEQFKPEYRKLNHDAIVPTLVDNGHVITQSLAIIEYLEETHPE
PALLPSKPADRAYVRSIVQQLACEIHPLNNLRVLKYLKRTVGVSDEVKDAWYHHWISSGFAALEEYLVADGRAGMLCFGD
TPTIADICLVPQVFNASRFNVDLSPYPTIRRICDYANSLDAFARAEPSVQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=211, Percent_Identity=52.132701421801, Blast_Score=204, Evalue=5e-53,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=50.625, Blast_Score=143, Evalue=9e-35,
Organism=Homo sapiens, GI194440732, Length=211, Percent_Identity=40.7582938388626, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=46.9194312796209, Blast_Score=173, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17510461, Length=212, Percent_Identity=43.8679245283019, Blast_Score=156, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17556142, Length=212, Percent_Identity=43.3962264150943, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=50.2369668246445, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=50.2369668246445, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=50.2369668246445, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=45.7547169811321, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17933730, Length=160, Percent_Identity=33.75, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17864594, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24267; Mature: 24267

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSYFRSSASYRVRIALNLKNLPYEYVPVHLLRDGGEQFKPEYRKLNHDAIVPTLVDN
CCHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCHHCCHHHHHCCCCCCCEEEECC
GHVITQSLAIIEYLEETHPEPALLPSKPADRAYVRSIVQQLACEIHPLNNLRVLKYLKRT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VGVSDEVKDAWYHHWISSGFAALEEYLVADGRAGMLCFGDTPTIADICLVPQVFNASRFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
VDLSPYPTIRRICDYANSLDAFARAEPSVQPDAE
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLYSYFRSSASYRVRIALNLKNLPYEYVPVHLLRDGGEQFKPEYRKLNHDAIVPTLVDN
CCHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCHHCCHHHHHCCCCCCCEEEECC
GHVITQSLAIIEYLEETHPEPALLPSKPADRAYVRSIVQQLACEIHPLNNLRVLKYLKRT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VGVSDEVKDAWYHHWISSGFAALEEYLVADGRAGMLCFGDTPTIADICLVPQVFNASRFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCEEE
VDLSPYPTIRRICDYANSLDAFARAEPSVQPDAE
EECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]