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The map label for this gene is amtB [H]

Identifier: 186475026

GI number: 186475026

Start: 300255

End: 301760

Strand: Direct

Name: amtB [H]

Synonym: Bphy_0257

Alternate gene names: 186475026

Gene position: 300255-301760 (Clockwise)

Preceding gene: 186475025

Following gene: 186475027

Centisome position: 8.63

GC content: 64.74

Gene sequence:

>1506_bases
ATGCGTAAATTATTGATGTCCCTGCTGATGGCCGGCTCGCTGCTGTCGGTCGGCATCGGTGCAGCCCTCGCGGACGACGC
GTCGGCGCCTGCCGCGGCTTCCGCCCCGGACGCGACCGCGAGCGCGCCGGCTGCATCCGATGCAGCCGCAGCGCCCGCGG
CGAGCGCACCTGCCGCCGACGCTTCCGCTGCACCGGCAGCCAGCGCCCCCGCCGACGCGTCGGCAGCGGCAGCCGCTTCG
GACGCTGCGCCCGCCGCGCCGACCGCACCGTTCTCGGTCGATTCGTCGAAGATCAGCTCGGGCGACACGGCCTGGATGCT
GACCTCCACGGCGCTCGTACTGTTCATGACGGTCCCCGGTCTCGCGCTCTTCTACGCCGGCATGGTCCGCAAGAAGAACG
TGCTTGCCACGGTCATGCAGAGCTTCGCGATCACCGCGCTCGTCACAGTGATCTGGACGGTGCTCGGCTACAGCCTCGCG
TTCACGCCGGGCGGCTCGTTCCTCGGCGGCTTCTCGCGCGTGATGCTGCACGGTATGGCATATATCAAGGGCGACAAAGC
GACGACGCTGACGGTCAGCCACCTCGCGCCGACCATTCCGGAATCGGTCTACTTCGTCTATCAGATGACGTTCGCGATCA
TCACGCCGGCACTGATTACGGGCGCGTTTGCTGACCGGATGAAGTTCTCGGCAATGCTGATCTTCATGACCCTGTGGTCG
ATCATCGTCTACTCGCCGATCGCGCACATGGTCTGGGAGCCGACGGGCTGGCTGGCGACGGCAGGCATCCTCGACTTCGC
GGGCGGCACGGTGGTGCACATCAACGCGGGTATCGCAGGTCTGGTTTGCTGTCTCGTGCTCGGCAAGCGCACGGGTTACG
GCAAGGATTCGATGGCGCCGCACAACCTCGTGCTGACGATGATCGGTGCATCGATGCTGTGGGTAGGCTGGTTCGGCTTC
AACGCGGGTTCGGCAGTCGCTGCTGACGGCCGTGCCGGTTTCGCGATGCTGACGACGCAGATTGCAACGGGCATTGCCGC
ACTGGGCTGGATGTTCGCCGAATGGATTGCGAAGGGTAAGCCGTCGGTGCTCGGTATCGCGTCGGGTGCTGTGGCCGGTC
TGGTTGCAATTACGCCCGCTTCGGGTTTCGTCGGCGTGGGCGGCGCGCTCGCCATCGGTCTGATCGCAGGCGTGGTGTGC
TTCTGGTCGGCAACGTGGCTCAAGCACAAGATGGGCTACGACGACTCGCTCGATGCGTTCGGCGTGCACTGCATCGGCGG
TATCGTCGGCGCAATCCTGACGGGCGTGTTCGCCGTCAAGGATATCGGCGGCTTCGACGGCAGCGTTCTGCTCCAGGCGA
AGGGCGTCGTGACGACGCTCGTATACAGCGGCGTGATCAGCTTCATTCTGCTGAAGATCATCGACATGACCATCGGCCTG
CGCGTGACGGAAGAAGAAGAACGCGAAGGTCTGGACGTGATCCTGCACGGCGAACACGTCGAATAA

Upstream 100 bases:

>100_bases
GAAAGATTTTCGTCACGCCGATCGAACAGGTGATTCGTATTCGTACCGGCGAGACCGGCGCGGACGCACTGTAAAAAACA
GCGACAAGAGGAAACTGAAG

Downstream 100 bases:

>100_bases
GGCAAATTTCCCCGCGGACGGGGTCCCGAAAGGTACCAGCGCAGCGACTTTGGCCCGCCTTCACGGCGGGCTTTTTTCAT
GCCGCGGCCGTCCGCCGCAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRKLLMSLLMAGSLLSVGIGAALADDASAPAAASAPDATASAPAASDAAAAPAASAPAADASAAPAASAPADASAAAAAS
DAAPAAPTAPFSVDSSKISSGDTAWMLTSTALVLFMTVPGLALFYAGMVRKKNVLATVMQSFAITALVTVIWTVLGYSLA
FTPGGSFLGGFSRVMLHGMAYIKGDKATTLTVSHLAPTIPESVYFVYQMTFAIITPALITGAFADRMKFSAMLIFMTLWS
IIVYSPIAHMVWEPTGWLATAGILDFAGGTVVHINAGIAGLVCCLVLGKRTGYGKDSMAPHNLVLTMIGASMLWVGWFGF
NAGSAVAADGRAGFAMLTTQIATGIAALGWMFAEWIAKGKPSVLGIASGAVAGLVAITPASGFVGVGGALAIGLIAGVVC
FWSATWLKHKMGYDDSLDAFGVHCIGGIVGAILTGVFAVKDIGGFDGSVLLQAKGVVTTLVYSGVISFILLKIIDMTIGL
RVTEEEEREGLDVILHGEHVE

Sequences:

>Translated_501_residues
MRKLLMSLLMAGSLLSVGIGAALADDASAPAAASAPDATASAPAASDAAAAPAASAPAADASAAPAASAPADASAAAAAS
DAAPAAPTAPFSVDSSKISSGDTAWMLTSTALVLFMTVPGLALFYAGMVRKKNVLATVMQSFAITALVTVIWTVLGYSLA
FTPGGSFLGGFSRVMLHGMAYIKGDKATTLTVSHLAPTIPESVYFVYQMTFAIITPALITGAFADRMKFSAMLIFMTLWS
IIVYSPIAHMVWEPTGWLATAGILDFAGGTVVHINAGIAGLVCCLVLGKRTGYGKDSMAPHNLVLTMIGASMLWVGWFGF
NAGSAVAADGRAGFAMLTTQIATGIAALGWMFAEWIAKGKPSVLGIASGAVAGLVAITPASGFVGVGGALAIGLIAGVVC
FWSATWLKHKMGYDDSLDAFGVHCIGGIVGAILTGVFAVKDIGGFDGSVLLQAKGVVTTLVYSGVISFILLKIIDMTIGL
RVTEEEEREGLDVILHGEHVE
>Mature_501_residues
MRKLLMSLLMAGSLLSVGIGAALADDASAPAAASAPDATASAPAASDAAAAPAASAPAADASAAPAASAPADASAAAAAS
DAAPAAPTAPFSVDSSKISSGDTAWMLTSTALVLFMTVPGLALFYAGMVRKKNVLATVMQSFAITALVTVIWTVLGYSLA
FTPGGSFLGGFSRVMLHGMAYIKGDKATTLTVSHLAPTIPESVYFVYQMTFAIITPALITGAFADRMKFSAMLIFMTLWS
IIVYSPIAHMVWEPTGWLATAGILDFAGGTVVHINAGIAGLVCCLVLGKRTGYGKDSMAPHNLVLTMIGASMLWVGWFGF
NAGSAVAADGRAGFAMLTTQIATGIAALGWMFAEWIAKGKPSVLGIASGAVAGLVAITPASGFVGVGGALAIGLIAGVVC
FWSATWLKHKMGYDDSLDAFGVHCIGGIVGAILTGVFAVKDIGGFDGSVLLQAKGVVTTLVYSGVISFILLKIIDMTIGL
RVTEEEEREGLDVILHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=49.5073891625616, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=26.0089686098655, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=434, Percent_Identity=26.036866359447, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=25.635103926097, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=24.390243902439, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=33.7209302325581, Blast_Score=222, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=425, Percent_Identity=33.6470588235294, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=31.7307692307692, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI161078314, Length=438, Percent_Identity=26.7123287671233, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50966; Mature: 50966

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLMSLLMAGSLLSVGIGAALADDASAPAAASAPDATASAPAASDAAAAPAASAPAAD
CHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAAPAASAPADASAAAAASDAAPAAPTAPFSVDSSKISSGDTAWMLTSTALVLFMTVPG
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LALFYAGMVRKKNVLATVMQSFAITALVTVIWTVLGYSLAFTPGGSFLGGFSRVMLHGMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
YIKGDKATTLTVSHLAPTIPESVYFVYQMTFAIITPALITGAFADRMKFSAMLIFMTLWS
HEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIVYSPIAHMVWEPTGWLATAGILDFAGGTVVHINAGIAGLVCCLVLGKRTGYGKDSMAP
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
HNLVLTMIGASMLWVGWFGFNAGSAVAADGRAGFAMLTTQIATGIAALGWMFAEWIAKGK
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSVLGIASGAVAGLVAITPASGFVGVGGALAIGLIAGVVCFWSATWLKHKMGYDDSLDAF
CCEEEECCHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GVHCIGGIVGAILTGVFAVKDIGGFDGSVLLQAKGVVTTLVYSGVISFILLKIIDMTIGL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
RVTEEEEREGLDVILHGEHVE
EECCHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MRKLLMSLLMAGSLLSVGIGAALADDASAPAAASAPDATASAPAASDAAAAPAASAPAAD
CHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASAAPAASAPADASAAAAASDAAPAAPTAPFSVDSSKISSGDTAWMLTSTALVLFMTVPG
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LALFYAGMVRKKNVLATVMQSFAITALVTVIWTVLGYSLAFTPGGSFLGGFSRVMLHGMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHH
YIKGDKATTLTVSHLAPTIPESVYFVYQMTFAIITPALITGAFADRMKFSAMLIFMTLWS
HEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIVYSPIAHMVWEPTGWLATAGILDFAGGTVVHINAGIAGLVCCLVLGKRTGYGKDSMAP
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
HNLVLTMIGASMLWVGWFGFNAGSAVAADGRAGFAMLTTQIATGIAALGWMFAEWIAKGK
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSVLGIASGAVAGLVAITPASGFVGVGGALAIGLIAGVVCFWSATWLKHKMGYDDSLDAF
CCEEEECCHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GVHCIGGIVGAILTGVFAVKDIGGFDGSVLLQAKGVVTTLVYSGVISFILLKIIDMTIGL
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
RVTEEEEREGLDVILHGEHVE
EECCHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]