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The map label for this gene is lipB

Identifier: 186474985

GI number: 186474985

Start: 238110

End: 238877

Strand: Direct

Name: lipB

Synonym: Bphy_0216

Alternate gene names: 186474985

Gene position: 238110-238877 (Clockwise)

Preceding gene: 186474984

Following gene: 186474986

Centisome position: 6.84

GC content: 64.19

Gene sequence:

>768_bases
ATGTGTGCCACGCCGGTTTCACCATCCCCCGAATCTCCGCGTTCCGCCCAAGCGGGCGCCGCTGCGTCCGACGCCGCTGC
GCAGCCTGTAATCGTTCGCTGGCGCGGCCTGGAAGACTATCAGGCCAGCTTCGACGCGATGCGCGCGTTCACCGGTTCGC
GCTCATTCGAAACCTCAGACGAAATCTGGCTCGTCGAACATCCGCCCGTTTTCACGCTTGGCCAGGCCGGCGACCCCGCG
CATCTGCTGGCCGCCGACAGCGGCATTCCGCTTGTGAAAGTCGATCGCGGGGGGCAGATCACCTATCACGGTCCCGGGCA
GGTCGTCGGCTACCTGCTGCTAGACTTGCGTCGCCGGAAACTGATGGTGCGCGAACTGGTCACCCGGATCGAGCAGGCCG
TGATCGACACACTCGCCGCGTATAATCTCGCCGGTGAACGCAAGGCAGGCGCGCCGGGTATCTATGTATCGCCCGAGCAG
CCGAATGCGGGGCTGCACGCCGGCGCAAAGATCGCCGCATTAGGTCTGAAGATTCGTAACGGTTGTAGCTATCACGGCGT
GAGCCTGAACGTGAAGATGGACCTGCAGCCGTTTCTCGCCATTAATCCGTGCGGTTACGCGGGGCTCGAAACAGTCGATA
TGGCGACGCTCGGCGTCGCGGCCGGCTGGGACGACGTAGCCCAAACCCTTGCAGCAAGCCTCATCGCCAACATCGACGGC
ATCCCCGCAGCCGTCGGCCTACCGCAGGCCGGTGCCATTACCGCCTGA

Upstream 100 bases:

>100_bases
GCTGCGCTCGGGATGGCGGACGGTCTGATTCACAAATCGATCCCACTTATTGCTCTCGCGATCGGCGAGGTGCGCGGGAT
TTCGGTAAACTGCCGCAATC

Downstream 100 bases:

>100_bases
CTGGAACGAACGAATGACTGACGTTACCGCGAATCCTGCAGCCTCGAGTCCCGTCAACGGCGCGGCCGGCGCCGCCCCCT
ACGACGCTACTGCCAAGCAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCATPVSPSPESPRSAQAGAAASDAAAQPVIVRWRGLEDYQASFDAMRAFTGSRSFETSDEIWLVEHPPVFTLGQAGDPA
HLLAADSGIPLVKVDRGGQITYHGPGQVVGYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVSPEQ
PNAGLHAGAKIAALGLKIRNGCSYHGVSLNVKMDLQPFLAINPCGYAGLETVDMATLGVAAGWDDVAQTLAASLIANIDG
IPAAVGLPQAGAITA

Sequences:

>Translated_255_residues
MCATPVSPSPESPRSAQAGAAASDAAAQPVIVRWRGLEDYQASFDAMRAFTGSRSFETSDEIWLVEHPPVFTLGQAGDPA
HLLAADSGIPLVKVDRGGQITYHGPGQVVGYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVSPEQ
PNAGLHAGAKIAALGLKIRNGCSYHGVSLNVKMDLQPFLAINPCGYAGLETVDMATLGVAAGWDDVAQTLAASLIANIDG
IPAAVGLPQAGAITA
>Mature_255_residues
MCATPVSPSPESPRSAQAGAAASDAAAQPVIVRWRGLEDYQASFDAMRAFTGSRSFETSDEIWLVEHPPVFTLGQAGDPA
HLLAADSGIPLVKVDRGGQITYHGPGQVVGYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVSPEQ
PNAGLHAGAKIAALGLKIRNGCSYHGVSLNVKMDLQPFLAINPCGYAGLETVDMATLGVAAGWDDVAQTLAASLIANIDG
IPAAVGLPQAGAITA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=211, Percent_Identity=49.2890995260664, Blast_Score=206, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323268, Length=171, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355483, Length=160, Percent_Identity=32.5, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BURP8 (B2JC45)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856455.1
- ProteinModelPortal:   B2JC45
- SMR:   B2JC45
- GeneID:   6241718
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0216
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14343
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26579; Mature: 26579

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCATPVSPSPESPRSAQAGAAASDAAAQPVIVRWRGLEDYQASFDAMRAFTGSRSFETSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC
EIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVKVDRGGQITYHGPGQVVGYLLLDLRRRK
CEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHH
LMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVSPEQPNAGLHAGAKIAALGLKIRN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEEECC
GCSYHGVSLNVKMDLQPFLAINPCGYAGLETVDMATLGVAAGWDDVAQTLAASLIANIDG
CCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IPAAVGLPQAGAITA
CCHHCCCCCCCCCCC
>Mature Secondary Structure
MCATPVSPSPESPRSAQAGAAASDAAAQPVIVRWRGLEDYQASFDAMRAFTGSRSFETSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCC
EIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVKVDRGGQITYHGPGQVVGYLLLDLRRRK
CEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHH
LMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVSPEQPNAGLHAGAKIAALGLKIRN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEEECC
GCSYHGVSLNVKMDLQPFLAINPCGYAGLETVDMATLGVAAGWDDVAQTLAASLIANIDG
CCCCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
IPAAVGLPQAGAITA
CCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA