Click here to switch to the map view.

The map label for this gene is lipA [H]

Identifier: 186474986

GI number: 186474986

Start: 238891

End: 239895

Strand: Direct

Name: lipA [H]

Synonym: Bphy_0217

Alternate gene names: 186474986

Gene position: 238891-239895 (Clockwise)

Preceding gene: 186474985

Following gene: 186474987

Centisome position: 6.87

GC content: 61.79

Gene sequence:

>1005_bases
ATGACTGACGTTACCGCGAATCCTGCAGCCTCGAGTCCCGTCAACGGCGCGGCCGGCGCCGCCCCCTACGACGCTACTGC
CAAGCAGAAGGCGCAAGCCAAGACTGCGCGCATTCCGATCAAGATCGTTCCGATCGAAAAACTGAAGAAGCCCGACTGGA
TCCGCGTGAAGGCCGCGACGGGCAATTCGCGTTTCTACGAGATCAAGCAGATTCTGCGCGAGCACAACCTGCACACGGTG
TGCGAAGAAGCCAGCTGCCCGAACATCGGCGAGTGCTTCGGCAAGGGCACGGCAACCTTCATGATCATGGGCGACAAATG
CACGCGCCGCTGCCCGTTCTGCGACGTCGGCCACGGTCGTCCAGATCCGCTCGATCCCGAGGAACCCGGCAACCTCGCGC
GCACGATCGCCGCGTTGAAGCTCAAGTACGTGGTGATTACCAGTGTGGACCGCGACGATCTGCGTGACGGCGGCGCGGCG
CACTTCGTCGAGTGCATTCAGAAGACGCGAGAGCTGTCGCCGGAAACGCGCATTGAAATCCTGACGCCCGACTTCCGCGG
CCGTCTCGATCGCGCGATCGGCATTTTGAACGCGGCCCCGCCCGACGTGATGAATCACAACCTCGAAACGGTGCCGCGGC
TGTATAAGGAAGCGCGCCCAGGTTCCGACTACGCGCATTCGCTGAAGCTGCTGAAGGACTTCAAGGCGCTGCATCCCAAT
GTCGCGACGAAGTCAGGCCTGATGGTCGGTCTCGGCGAGACGGAAGAAGAAATCCTGCAGGTGATGCGCGATCTGCGCGA
GCATAACGTCGACATGCTGACGATCGGCCAGTATCTGCAGCCGTCCGAGCACCACCTGCCCGTGCGTGCGTACGTGCATC
CCGATACGTTCAGGATGTACGAGGAAGAAGCGTACAAGATGGGCTTTACGCACGCGGCCGTCGGCGCAATGGTGCGTTCG
AGCTATCACGCCGATGTGCAGGCACACGATGCCGGAGTGGTCTGA

Upstream 100 bases:

>100_bases
CAAACCCTTGCAGCAAGCCTCATCGCCAACATCGACGGCATCCCCGCAGCCGTCGGCCTACCGCAGGCCGGTGCCATTAC
CGCCTGACTGGAACGAACGA

Downstream 100 bases:

>100_bases
ACGACATTCACAGCAGCAACCGCTTGAAGAAAAAGCTCGCGAAAGCGGGCTTTTTTGTTTTCTGATCGACGTCCCAGCCG
AAACACAATCTGTTGCAAAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTDVTANPAASSPVNGAAGAAPYDATAKQKAQAKTARIPIKIVPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTV
CEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPEEPGNLARTIAALKLKYVVITSVDRDDLRDGGAA
HFVECIQKTRELSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPN
VATKSGLMVGLGETEEEILQVMRDLREHNVDMLTIGQYLQPSEHHLPVRAYVHPDTFRMYEEEAYKMGFTHAAVGAMVRS
SYHADVQAHDAGVV

Sequences:

>Translated_334_residues
MTDVTANPAASSPVNGAAGAAPYDATAKQKAQAKTARIPIKIVPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTV
CEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPEEPGNLARTIAALKLKYVVITSVDRDDLRDGGAA
HFVECIQKTRELSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPN
VATKSGLMVGLGETEEEILQVMRDLREHNVDMLTIGQYLQPSEHHLPVRAYVHPDTFRMYEEEAYKMGFTHAAVGAMVRS
SYHADVQAHDAGVV
>Mature_333_residues
TDVTANPAASSPVNGAAGAAPYDATAKQKAQAKTARIPIKIVPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPEEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAH
FVECIQKTRELSPETRIEILTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPNV
ATKSGLMVGLGETEEEILQVMRDLREHNVDMLTIGQYLQPSEHHLPVRAYVHPDTFRMYEEEAYKMGFTHAAVGAMVRSS
YHADVQAHDAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=47.2027972027972, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=48.4, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786846, Length=304, Percent_Identity=63.1578947368421, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=47.244094488189, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=324, Percent_Identity=41.9753086419753, Blast_Score=249, Evalue=5e-67,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=46.5034965034965, Blast_Score=256, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36945; Mature: 36813

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVTANPAASSPVNGAAGAAPYDATAKQKAQAKTARIPIKIVPIEKLKKPDWIRVKAAT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEC
GNSRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGR
CCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
PDPLDPEEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIQKTRELSPETRIEI
CCCCCCCCCHHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPN
ECCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
VATKSGLMVGLGETEEEILQVMRDLREHNVDMLTIGQYLQPSEHHLPVRAYVHPDTFRMY
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEEEECCHHHHHH
EEEAYKMGFTHAAVGAMVRSSYHADVQAHDAGVV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TDVTANPAASSPVNGAAGAAPYDATAKQKAQAKTARIPIKIVPIEKLKKPDWIRVKAAT
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEC
GNSRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGR
CCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
PDPLDPEEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIQKTRELSPETRIEI
CCCCCCCCCHHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGRLDRAIGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPN
ECCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
VATKSGLMVGLGETEEEILQVMRDLREHNVDMLTIGQYLQPSEHHLPVRAYVHPDTFRMY
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEEEECCHHHHHH
EEEAYKMGFTHAAVGAMVRSSYHADVQAHDAGVV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA