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The map label for this gene is 186474927

Identifier: 186474927

GI number: 186474927

Start: 179525

End: 180415

Strand: Direct

Name: 186474927

Synonym: Bphy_0158

Alternate gene names: NA

Gene position: 179525-180415 (Clockwise)

Preceding gene: 186474925

Following gene: 186474929

Centisome position: 5.16

GC content: 64.42

Gene sequence:

>891_bases
ATGCCCTTCGCAGACTTCACCCCGTTTCGGTTCACGGCTGGGGACGTCGATATTTTCGGAGTCAAGGGCGGAGCGGGGCC
GCCTTTGCTGCTGCTGCACGGTCACCCGCAGACCCACCTGATCTGGCATCGATGTGCGTCGCAGCTCGCCGGGCATTTCA
CGGTCATCGCGACGGACCTGCGCGGCTACGGCGCGTCGGCGAAACCGCCGAGCGACGCGACACACGCACCGTATTCAAAA
CGTGCGATGGCCGCCGATCAGGTCGCGGTGATGCGTCATTTCGGTTACGAGCGCTTTCTCGTCTGCGCGCACGACCGTGG
CGCGCGCGTCGCACACCGGATGGCGCTCGATTTCCCCGATTTCGTCGAACGCCTGATGCTGCTCGACATTGCGCCGACGC
TCGCCATGTACGAAGCTACCGACCGCGCCTTCGCGACGGCCTATTTCCACTGGTTTTTTCTGATCCAACCCGAGCCGCTG
CCGGAGACGCTCATCGAAGGCAATCCCGATGTCTACGTCGACCGTGTGATGGGCAGCCGTGCGGCAGGCCTCGCGCCATT
CGCGCCCGCAGTGCTCGCCGAGTATCGCAATGCGTTGCGGCAACCGGGCGCCGTGCACGCGATGTGCGAGGATTATCGTG
CGTCGGCGACCATCGACCTCGAGCATGATCGTGCGGCTGTCGAGCGCGGTCACAAGATCGCGTGTCCGGTACGCGTGCTG
TGGGGCGCGCAAGGTGTGATCGAGCGTTGCTTCGAGCCGATGGCCGAATGGCGCAAGGTCGCGCGCGATGTCAGCGGACG
CTCGCTGGCCTGTGGGCATTACATCCCCGAAGAAGCACCCGGGGAACTCGTGCCGGAAATGCTGTCGTTCTTCGAAGCCG
TCGAGCTTTGA

Upstream 100 bases:

>100_bases
GAAGGAGAGTTCAGGTTCTAGGAAAGCGTGGGTTAGCACACTCGCGGCGATATCCACGCCTTCTACAATGCCCAGACTTA
CAACGAGAGCGAGACCTGAC

Downstream 100 bases:

>100_bases
TTCCCTTCTGCGCCCGTGCCGCCGCCCGTTCAGGCGAGCGGCGGCAGGCGCCGCGATACCGGTGTCGACTTGACGATGGC
GGTGCTCGTTTCTGCACGCT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPFADFTPFRFTAGDVDIFGVKGGAGPPLLLLHGHPQTHLIWHRCASQLAGHFTVIATDLRGYGASAKPPSDATHAPYSK
RAMAADQVAVMRHFGYERFLVCAHDRGARVAHRMALDFPDFVERLMLLDIAPTLAMYEATDRAFATAYFHWFFLIQPEPL
PETLIEGNPDVYVDRVMGSRAAGLAPFAPAVLAEYRNALRQPGAVHAMCEDYRASATIDLEHDRAAVERGHKIACPVRVL
WGAQGVIERCFEPMAEWRKVARDVSGRSLACGHYIPEEAPGELVPEMLSFFEAVEL

Sequences:

>Translated_296_residues
MPFADFTPFRFTAGDVDIFGVKGGAGPPLLLLHGHPQTHLIWHRCASQLAGHFTVIATDLRGYGASAKPPSDATHAPYSK
RAMAADQVAVMRHFGYERFLVCAHDRGARVAHRMALDFPDFVERLMLLDIAPTLAMYEATDRAFATAYFHWFFLIQPEPL
PETLIEGNPDVYVDRVMGSRAAGLAPFAPAVLAEYRNALRQPGAVHAMCEDYRASATIDLEHDRAAVERGHKIACPVRVL
WGAQGVIERCFEPMAEWRKVARDVSGRSLACGHYIPEEAPGELVPEMLSFFEAVEL
>Mature_295_residues
PFADFTPFRFTAGDVDIFGVKGGAGPPLLLLHGHPQTHLIWHRCASQLAGHFTVIATDLRGYGASAKPPSDATHAPYSKR
AMAADQVAVMRHFGYERFLVCAHDRGARVAHRMALDFPDFVERLMLLDIAPTLAMYEATDRAFATAYFHWFFLIQPEPLP
ETLIEGNPDVYVDRVMGSRAAGLAPFAPAVLAEYRNALRQPGAVHAMCEDYRASATIDLEHDRAAVERGHKIACPVRVLW
GAQGVIERCFEPMAEWRKVARDVSGRSLACGHYIPEEAPGELVPEMLSFFEAVEL

Specific function: Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has low activity towards chloroacetate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777840, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI13376164, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI218777837, Length=232, Percent_Identity=24.5689655172414, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.3 [H]

Molecular weight: Translated: 32772; Mature: 32641

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFADFTPFRFTAGDVDIFGVKGGAGPPLLLLHGHPQTHLIWHRCASQLAGHFTVIATDL
CCCCCCCCCEEECCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEC
RGYGASAKPPSDATHAPYSKRAMAADQVAVMRHFGYERFLVCAHDRGARVAHRMALDFPD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHH
FVERLMLLDIAPTLAMYEATDRAFATAYFHWFFLIQPEPLPETLIEGNPDVYVDRVMGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHCCCCCEEEEHHHCCC
AAGLAPFAPAVLAEYRNALRQPGAVHAMCEDYRASATIDLEHDRAAVERGHKIACPVRVL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHCCCEEECCHHEE
WGAQGVIERCFEPMAEWRKVARDVSGRSLACGHYIPEEAPGELVPEMLSFFEAVEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFADFTPFRFTAGDVDIFGVKGGAGPPLLLLHGHPQTHLIWHRCASQLAGHFTVIATDL
CCCCCCCCEEECCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEC
RGYGASAKPPSDATHAPYSKRAMAADQVAVMRHFGYERFLVCAHDRGARVAHRMALDFPD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHH
FVERLMLLDIAPTLAMYEATDRAFATAYFHWFFLIQPEPLPETLIEGNPDVYVDRVMGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHHHCCCCCEEEEHHHCCC
AAGLAPFAPAVLAEYRNALRQPGAVHAMCEDYRASATIDLEHDRAAVERGHKIACPVRVL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHCCCEEECCHHEE
WGAQGVIERCFEPMAEWRKVARDVSGRSLACGHYIPEEAPGELVPEMLSFFEAVEL
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA