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The map label for this gene is yafC [C]

Identifier: 186474924

GI number: 186474924

Start: 173794

End: 174732

Strand: Direct

Name: yafC [C]

Synonym: Bphy_0155

Alternate gene names: 186474924

Gene position: 173794-174732 (Clockwise)

Preceding gene: 186474923

Following gene: 186474925

Centisome position: 5.0

GC content: 65.18

Gene sequence:

>939_bases
ATGGATACCCTCGTCAGCATGAAAGTGTTTCGCCACGTCGTCGAGGTCGGCAGCTTTGTCGGCGCGTCGGAGAAAATGGA
CATGTCGGCGGCGATGGCGAGCAAGCATGTGATGCATCTCGAACAGCAGCTCGGCGCGCGGCTGTTGAATCGCACCACGC
GCCGCGTCGCGCCGACCGAGGCGGGCCGCGAGTACTACGAGCGCCTGAGCCAGGTGTTGAGCGAACTAGACGAAGCGGAA
CAGGCAGTGGGCGCCGCGAGCGTCGTGCCGCAAGGGCGGTTGCGCGTGTCGTCGCTATCGGCGTTCGGACTGCGCCATGT
GATGAGCGCCGTCGCCGATTATGCGACGCAGCATCCTCAGGTCACCGTCGACATCACGCTGTCGGACCGCGTTGTCGAAC
TGATCGACGAAGGCTTCGATGTCGCGATACGCGCGTCGCCGTCGGGACTCAAGTCGTCCTCGCTGATCGCGCGTCAGGTC
GCGACTGCGCACCTGGTGCTGTGTGCGTCGCCGGAATATATCGAGCGGCACGGCGCACCGAAATCTGCCGCTGATCTGAC
ACGCCACAACATCATTCAGTACTCAGGCGTATCGGCGCTCGAACTCGTGGCGGGCGCGAATGCCGAGAGCGGGCGCGTCA
AGCTGTCGGGCAATCTGATCGTCAATCATCTGGAGGCGCAGCGCGTCGTTGTGCTGCAGGGCGCGGCGCTCGCGCTGCTC
GGCACCGAAGTTATCGGCGACGATCTCGCGGCGGGCCGCGTCGTGCCGTTGCTGATCGACGAACTGCCGCCGCGCGAGCT
GCCGATCCACGTCGTCTACGCGAGCCGCCGGCACCTGTCGGCGAAGGTGCGCTCGTTCGTCGATTTCATCGCGGAGCGTT
TTTCAAGCGAGCCGCTCTGGCCGACGCTCGAGCAGATCAAGGCGCTCGCGCTGCGCTGA

Upstream 100 bases:

>100_bases
GGGCTTGTTCTTTCGGCTGCACCTACTGCCTCACATTGATGACCGCTCCTTGATAAAGTCGGGAATATCAGCCCGATTCA
TTCCTATTCCGAGCGACGCA

Downstream 100 bases:

>100_bases
CACGCGCGTCGCCCGTCGATTCACGCTGGGTGAACGGCGCACGGACGATTGGGTCGTATCGTTATACTGCTTGCAGCACC
CGCGAAGCACGAGACGAACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDTLVSMKVFRHVVEVGSFVGASEKMDMSAAMASKHVMHLEQQLGARLLNRTTRRVAPTEAGREYYERLSQVLSELDEAE
QAVGAASVVPQGRLRVSSLSAFGLRHVMSAVADYATQHPQVTVDITLSDRVVELIDEGFDVAIRASPSGLKSSSLIARQV
ATAHLVLCASPEYIERHGAPKSAADLTRHNIIQYSGVSALELVAGANAESGRVKLSGNLIVNHLEAQRVVVLQGAALALL
GTEVIGDDLAAGRVVPLLIDELPPRELPIHVVYASRRHLSAKVRSFVDFIAERFSSEPLWPTLEQIKALALR

Sequences:

>Translated_312_residues
MDTLVSMKVFRHVVEVGSFVGASEKMDMSAAMASKHVMHLEQQLGARLLNRTTRRVAPTEAGREYYERLSQVLSELDEAE
QAVGAASVVPQGRLRVSSLSAFGLRHVMSAVADYATQHPQVTVDITLSDRVVELIDEGFDVAIRASPSGLKSSSLIARQV
ATAHLVLCASPEYIERHGAPKSAADLTRHNIIQYSGVSALELVAGANAESGRVKLSGNLIVNHLEAQRVVVLQGAALALL
GTEVIGDDLAAGRVVPLLIDELPPRELPIHVVYASRRHLSAKVRSFVDFIAERFSSEPLWPTLEQIKALALR
>Mature_312_residues
MDTLVSMKVFRHVVEVGSFVGASEKMDMSAAMASKHVMHLEQQLGARLLNRTTRRVAPTEAGREYYERLSQVLSELDEAE
QAVGAASVVPQGRLRVSSLSAFGLRHVMSAVADYATQHPQVTVDITLSDRVVELIDEGFDVAIRASPSGLKSSSLIARQV
ATAHLVLCASPEYIERHGAPKSAADLTRHNIIQYSGVSALELVAGANAESGRVKLSGNLIVNHLEAQRVVVLQGAALALL
GTEVIGDDLAAGRVVPLLIDELPPRELPIHVVYASRRHLSAKVRSFVDFIAERFSSEPLWPTLEQIKALALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=32.5342465753425, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=34.5637583892617, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=32.8859060402685, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=29.6296296296296, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=27.681660899654, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=27.7397260273973, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=26.3698630136986, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788481, Length=307, Percent_Identity=25.0814332247557, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33791; Mature: 33791

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMKVFRHVVEVGSFVGASEKMDMSAAMASKHVMHLEQQLGARLLNRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLSQVLSELDEAEQAVGAASVVPQGRLRVSSLSAFGLRHVMSAVADYATQHPQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
VTVDITLSDRVVELIDEGFDVAIRASPSGLKSSSLIARQVATAHLVLCASPEYIERHGAP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEECCHHHHHHCCCC
KSAADLTRHNIIQYSGVSALELVAGANAESGRVKLSGNLIVNHLEAQRVVVLQGAALALL
CHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCEEEEECCCCEEEEECCCHHHHH
GTEVIGDDLAAGRVVPLLIDELPPRELPIHVVYASRRHLSAKVRSFVDFIAERFSSEPLW
HHHHHCCCHHCCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
PTLEQIKALALR
HHHHHHHHHHCC
>Mature Secondary Structure
MDTLVSMKVFRHVVEVGSFVGASEKMDMSAAMASKHVMHLEQQLGARLLNRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLSQVLSELDEAEQAVGAASVVPQGRLRVSSLSAFGLRHVMSAVADYATQHPQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
VTVDITLSDRVVELIDEGFDVAIRASPSGLKSSSLIARQVATAHLVLCASPEYIERHGAP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHEEEECCHHHHHHCCCC
KSAADLTRHNIIQYSGVSALELVAGANAESGRVKLSGNLIVNHLEAQRVVVLQGAALALL
CHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCEEEEECCCCEEEEECCCHHHHH
GTEVIGDDLAAGRVVPLLIDELPPRELPIHVVYASRRHLSAKVRSFVDFIAERFSSEPLW
HHHHHCCCHHCCCCHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
PTLEQIKALALR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]