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The map label for this gene is gst [C]

Identifier: 186474913

GI number: 186474913

Start: 161632

End: 162249

Strand: Direct

Name: gst [C]

Synonym: Bphy_0144

Alternate gene names: 186474913

Gene position: 161632-162249 (Clockwise)

Preceding gene: 186474911

Following gene: 186474922

Centisome position: 4.65

GC content: 62.3

Gene sequence:

>618_bases
ATGAAACTGTACTATTGGCCCAAGACCCGAGCATTCCGCGCCCTCTGGATGCTCGAAGAAGCATCCGCCCGCTACGAGCT
GGCCCATGTGAACATCCGGGCAGGCGAGCAGGACACCACTCAGTTCCACCACATCAACCCGATGTGCAAGCTTCCCGCGC
TCGACGACGACGGCGTCCAGGTCGCCGAATCCGGCGCGGTGCTGCTCTATCTCGCCGATCGTTTCCCCGAAGCCAATCTG
GGCGCACCAGTCGGCGATCCGTTGCGGGGACGTTTCCTTCAGTGGCTGTTCTTCACGCCGGGCTGTCTCGAACCGGCGAT
GGCCGAGAAGCTCACAGGTGCATCGGGCAATTCGTTCAGCTTCGGCTGGGGCAATCTCGAACGCGTGAAAGCCGCCATCG
ATATGGCGCTCTACGAAGGCGAATGGCTCCTCGGCGAACGCTTCTCCGCTGCCGATCTGCTGCTCGCGGGCACACTCCAG
ATCGCGTTCGTCGCGAAGCTGCTGGAGCCGACGGGGCGCATAGGCGAATATGTCGACCGGGCGATCTCGCGTGACGCGCA
CACGCGCGCGATGGCCATCGAGCAACGCGAAATCGAAGTATTGAAGCTGAAGCACTAG

Upstream 100 bases:

>100_bases
GCGAAACGCGGATGCCAGCGAAAAAACCAAAGGCATCGCCGTGCGATCCAGGCTACCCTAATCGCACAGTCATCCCGGCG
CCGAAAGGCAACTCAAAACG

Downstream 100 bases:

>100_bases
CCGCCACGCCGGGCGACGGCGCAGCACTCAACCCGTCGGCGCCTGCTGTAGTTCGTGCGGCTGGAGCGGCTCGACATCGT
CGAAATGCGTGTAGTCGATG

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-Glutathione; S-(2,4-dinitrophenyl)glutathione [C]

Alternate protein names: Glutathione S-Transferase Domain-Containing Protein; Glutathione S-Transferase-Like Protein; Glutathione S-Transferase Domain Protein; Glutathione S-Transferase-Like; Glutathione S-Transferase N-Terminal Domain Protein; Glutathione Transferase; Glutathione S-Transferase Domain; Glutathione S-Transferase Protein; Glutathione S-Transferase Family Protein; Glutathionine S-Transferase; Glutathione S-Transferase N-Terminal Domain-Containing; Glutathione S-Transferase N-Terminal Domain; Transcriptional Regulator; Glutathione Transferase Protein; Glutathione S-Transferase YfcG; GSH-Dependent Dehydroascorbate Reductase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKLYYWPKTRAFRALWMLEEASARYELAHVNIRAGEQDTTQFHHINPMCKLPALDDDGVQVAESGAVLLYLADRFPEANL
GAPVGDPLRGRFLQWLFFTPGCLEPAMAEKLTGASGNSFSFGWGNLERVKAAIDMALYEGEWLLGERFSAADLLLAGTLQ
IAFVAKLLEPTGRIGEYVDRAISRDAHTRAMAIEQREIEVLKLKH

Sequences:

>Translated_205_residues
MKLYYWPKTRAFRALWMLEEASARYELAHVNIRAGEQDTTQFHHINPMCKLPALDDDGVQVAESGAVLLYLADRFPEANL
GAPVGDPLRGRFLQWLFFTPGCLEPAMAEKLTGASGNSFSFGWGNLERVKAAIDMALYEGEWLLGERFSAADLLLAGTLQ
IAFVAKLLEPTGRIGEYVDRAISRDAHTRAMAIEQREIEVLKLKH
>Mature_205_residues
MKLYYWPKTRAFRALWMLEEASARYELAHVNIRAGEQDTTQFHHINPMCKLPALDDDGVQVAESGAVLLYLADRFPEANL
GAPVGDPLRGRFLQWLFFTPGCLEPAMAEKLTGASGNSFSFGWGNLERVKAAIDMALYEGEWLLGERFSAADLLLAGTLQ
IAFVAKLLEPTGRIGEYVDRAISRDAHTRAMAIEQREIEVLKLKH

Specific function: Conjugation Of Reduced Glutathione To A Wide Number Of Exogenous And Endogenous Hydrophobic Electrophiles. [C]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787923, Length=198, Percent_Identity=26.2626262626263, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788640, Length=168, Percent_Identity=30.3571428571429, Blast_Score=60, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20130123, Length=187, Percent_Identity=27.807486631016, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.18 [C]

Molecular weight: Translated: 22954; Mature: 22954

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYYWPKTRAFRALWMLEEASARYELAHVNIRAGEQDTTQFHHINPMCKLPALDDDGVQ
CEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCE
VAESGAVLLYLADRFPEANLGAPVGDPLRGRFLQWLFFTPGCLEPAMAEKLTGASGNSFS
EECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
FGWGNLERVKAAIDMALYEGEWLLGERFSAADLLLAGTLQIAFVAKLLEPTGRIGEYVDR
CCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AISRDAHTRAMAIEQREIEVLKLKH
HHCCCHHHHHHHHHHCCEEEEEECC
>Mature Secondary Structure
MKLYYWPKTRAFRALWMLEEASARYELAHVNIRAGEQDTTQFHHINPMCKLPALDDDGVQ
CEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCE
VAESGAVLLYLADRFPEANLGAPVGDPLRGRFLQWLFFTPGCLEPAMAEKLTGASGNSFS
EECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCC
FGWGNLERVKAAIDMALYEGEWLLGERFSAADLLLAGTLQIAFVAKLLEPTGRIGEYVDR
CCCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AISRDAHTRAMAIEQREIEVLKLKH
HHCCCHHHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; Glutathione; 1-chloro-2,4-dinitrobenzene; reduced glutathione [C]

Specific reaction: RX + Glutathione = HX + R-S-Glutathione 1-chloro-2,4-dinitrobenzene + reduced glutathione = S-(2,4-dinitrophenyl)glutathione [C]

General reaction: Aryl group transfer [C]

Inhibitor: Cd2+; CibacronBlue; Co2+; Cu2+; Hematin; Hg2+; Ni2+; Sulfophthaleins; Tributyl tinacetate; Triethyl tinbromide; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA