Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 186474825

GI number: 186474825

Start: 62165

End: 63658

Strand: Direct

Name: gatA

Synonym: Bphy_0054

Alternate gene names: 186474825

Gene position: 62165-63658 (Clockwise)

Preceding gene: 186474824

Following gene: 186474826

Centisome position: 1.79

GC content: 65.93

Gene sequence:

>1494_bases
ATGCATGAGAAAAGCTTGACCGAACTGCGCGCCGCGCTCGACGCGAAGCAGTGCTCCGCAGTCGAACTGGCGCAGACGTA
TCTGAAGCGGATCGAAGACCGCAAGGCGCTGAACGCGTTCGTTCACGTCGACGCGCAGCAGACGCTCGCGCAGGCGCAAG
CCGCCGACGCGCTGATCGCGGCCGGCAACGCCGGTCCGCTGACGGGTTTGCCCATCGCGCATAAAGACGTGTTCGTTACA
CGCAACTGGCGTTCCACGGCCGGCTCGAAGATGCTCGAAAACTACACGAGCCCGTTCGATGCCACCGTCGTCGACCGTCT
CGCGCAGGCGGGCATGGTGTGCGTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAAAACTCGTATT
TCGGCCCGGTGCAGAACCCGTGGGACCGCAAGGCGGTGCCGGGCGGCTCGTCGGGCGGTTCGGCGGCGGCTGTCGCCGCG
CGCCTCGCGCCCGCCGCGACGGGCACCGACACGGGCGGATCGATCCGTCAGCCCGCGTCGTTCTCGGGTATCACGGGCAT
CAAACCGACGTACGGCCGCGTATCGCGCTATGGGATGATCGCGTTCGCATCGTCGCTCGATCAGGGCGGCCCGATGGCGC
AGACGGCCGCGGACTGCGCGCTGCTGCTCAACGCCATGGGCGGCTTCGACGAGCGCGATTCGACGAGCCTCACGCACGAT
CACGAAGACTACACGCGCTATCTCGGCCAGGACTGGTCGGGCGCGGGCAATGCGCGCGACAAGCCGCTCGCGGGTCTGCG
GGTCGGCTTGCCGAAGGAATACTTCGGCGCAGGTCTGGCCGACGACGTGCGCGCATCGATCGACGCCGCGCTGAAGCAAT
ACGAAGCGCTTGGCGCAACGCTCGTCGAGGTGTCGCTGCCCAAGACCGAGCTGTCGATTCCCGTGTATTACGTGATCGCG
CCGGCGGAAGCATCGTCCAACCTGTCGCGGTTCGACGGCGTGCGCTACGGCCACCGCGCGGCGGAGTATCGCGATCTGCT
CGACATGTACAAGAAGTCGCGCGCAGAGGGTTTCGGCCCCGAAGTGAAGCGTCGCATTCTGGTCGGCACGTATGTGCTGT
CGCACGGCTACTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGCAGGATTTCCAGGAAGCGTTC
AGGCATTGCGACGTGATCATGGGCCCGGTGGCGCCGTCGGTCGCGTGGGATATCGGTGCGAAGGGCGACGACCCCGTGCA
GATGTACCTCGCGGACATCTACACGCTGTCGGTGAGCCTTGCCGGTTTGCCCGGCATGAGCGTACCTTGCGGCTTCGGCG
CAGGCGCGAATGCGCAGCGTCCGGTCGGCTTGCAGATCATCGGCAACTATTTCAACGAAGCACGCATGCTGCAAGTGGCC
GACGCGTTCCAGCGCGCCACCGACTGGCATCGCATGGCGCCGGCGGGAGTCTGA

Upstream 100 bases:

>100_bases
CGCGCGACATGGCACGCTCCTTTCGGCCCGGCGAGCCGGCGGGCGGCCGGATGCCCAAACGGCGGCGCACTGCGCCGTCG
CGCTTTCCAGGAAAAACGCA

Downstream 100 bases:

>100_bases
TATGGCAACACAATGGGAAGTGGTTATCGGTCTCGAGACCCACGCGCAACTGTCGACGGTATCGAAAATCTTCTCGGGCG
CGGCCACGCAGTTCGGCGCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MHEKSLTELRAALDAKQCSAVELAQTYLKRIEDRKALNAFVHVDAQQTLAQAQAADALIAAGNAGPLTGLPIAHKDVFVT
RNWRSTAGSKMLENYTSPFDATVVDRLAQAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQTAADCALLLNAMGGFDERDSTSLTHD
HEDYTRYLGQDWSGAGNARDKPLAGLRVGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIA
PAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAF
RHCDVIMGPVAPSVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVA
DAFQRATDWHRMAPAGV

Sequences:

>Translated_497_residues
MHEKSLTELRAALDAKQCSAVELAQTYLKRIEDRKALNAFVHVDAQQTLAQAQAADALIAAGNAGPLTGLPIAHKDVFVT
RNWRSTAGSKMLENYTSPFDATVVDRLAQAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQTAADCALLLNAMGGFDERDSTSLTHD
HEDYTRYLGQDWSGAGNARDKPLAGLRVGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIA
PAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAF
RHCDVIMGPVAPSVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVA
DAFQRATDWHRMAPAGV
>Mature_497_residues
MHEKSLTELRAALDAKQCSAVELAQTYLKRIEDRKALNAFVHVDAQQTLAQAQAADALIAAGNAGPLTGLPIAHKDVFVT
RNWRSTAGSKMLENYTSPFDATVVDRLAQAGMVCVGKTNMDEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAA
RLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQTAADCALLLNAMGGFDERDSTSLTHD
HEDYTRYLGQDWSGAGNARDKPLAGLRVGLPKEYFGAGLADDVRASIDAALKQYEALGATLVEVSLPKTELSIPVYYVIA
PAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGPEVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAF
RHCDVIMGPVAPSVAWDIGAKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVA
DAFQRATDWHRMAPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=36.7704280155642, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=24.1516966067864, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI166795287, Length=148, Percent_Identity=37.8378378378378, Blast_Score=76, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=38.1818181818182, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=30.672268907563, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=226, Percent_Identity=31.858407079646, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=324, Percent_Identity=26.5432098765432, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=444, Percent_Identity=35.5855855855856, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=483, Percent_Identity=26.7080745341615, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=193, Percent_Identity=29.5336787564767, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=38.5858585858586, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24652985, Length=510, Percent_Identity=25.0980392156863, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922090, Length=510, Percent_Identity=25.0980392156863, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652981, Length=510, Percent_Identity=25.0980392156863, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652983, Length=510, Percent_Identity=25.0980392156863, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=24.0234375, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=25.8126195028681, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=513, Percent_Identity=24.7563352826511, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24648435, Length=513, Percent_Identity=24.7563352826511, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648437, Length=513, Percent_Identity=24.7563352826511, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=445, Percent_Identity=25.3932584269663, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=445, Percent_Identity=25.3932584269663, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_BURP8 (B2JJW2)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856295.1
- ProteinModelPortal:   B2JJW2
- SMR:   B2JJW2
- GeneID:   6241558
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0054
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53263; Mature: 53263

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 75-75 ACT_SITE 150-150 ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKSLTELRAALDAKQCSAVELAQTYLKRIEDRKALNAFVHVDAQQTLAQAQAADALIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHEEE
AGNAGPLTGLPIAHKDVFVTRNWRSTAGSKMLENYTSPFDATVVDRLAQAGMVCVGKTNM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCC
DEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQTAADCALLLNAMGGFDERDSTSLTHD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HEDYTRYLGQDWSGAGNARDKPLAGLRVGLPKEYFGAGLADDVRASIDAALKQYEALGAT
HHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCE
LVEVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGP
EEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
EVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRHCDVIMGPVAPSVAWDIGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCC
KGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAFQRATDWHRMAPAGV
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHEKSLTELRAALDAKQCSAVELAQTYLKRIEDRKALNAFVHVDAQQTLAQAQAADALIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHEEE
AGNAGPLTGLPIAHKDVFVTRNWRSTAGSKMLENYTSPFDATVVDRLAQAGMVCVGKTNM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCC
DEFAMGSSNENSYFGPVQNPWDRKAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
FSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAQTAADCALLLNAMGGFDERDSTSLTHD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HEDYTRYLGQDWSGAGNARDKPLAGLRVGLPKEYFGAGLADDVRASIDAALKQYEALGAT
HHHHHHHHCCCCCCCCCCCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCE
LVEVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAAEYRDLLDMYKKSRAEGFGP
EEEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
EVKRRILVGTYVLSHGYYDAYYLQAQKIRRIIAQDFQEAFRHCDVIMGPVAPSVAWDIGA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCC
KGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAQRPVGLQIIGNYFNEARMLQVA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAFQRATDWHRMAPAGV
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA