Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 186474826

GI number: 186474826

Start: 63660

End: 65135

Strand: Direct

Name: gatB [H]

Synonym: Bphy_0055

Alternate gene names: 186474826

Gene position: 63660-65135 (Clockwise)

Preceding gene: 186474825

Following gene: 186474827

Centisome position: 1.83

GC content: 61.72

Gene sequence:

>1476_bases
ATGGCAACACAATGGGAAGTGGTTATCGGTCTCGAGACCCACGCGCAACTGTCGACGGTATCGAAAATCTTCTCGGGCGC
GGCCACGCAGTTCGGCGCCGCGCCGAACACGCAGGCCTGCCCCGTCGACCTGGCCCTTCCGGGCGTGCTGCCCGTGATGA
ACAAAGGCGCGGTGGAGCGCGCAATCCAGTTCGGTCTCGCGATCGATGCGACCATCGCGCCGCGCAGCATCTTCGCGCGC
AAGAACTACTTCTACCCTGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAAATCCCCGTCGTGCAGGGCGGCCAGAT
CACGATTCAGGTTCCCGCCAATGAAAAGGCCGGAAAAGAAGCGTACGAGAAGACCATCAACCTGACACGTGCTCACCTCG
AAGAAGATGCGGGCAAGTCGCTGCACGAAGACTTCGCGGGCATGACGGGCATCGACCTGAATCGCGCGGGCACGCCGCTG
CTCGAAATCGTTACCGAACCGGAAATGCGCAGCGCCGCCGAGGCCGTTGCCTACGCGAAGGCGCTACATGGTCTGGTGGT
GTGGCTCGGCATTTGCGATGGCAACATGCAGGAAGGCTCGTTCCGCTGTGACGCGAACGTGTCGGTGCGTCCCGTGGGGC
AAAAAGAATTCGGCACGCGCGCCGAAATCAAGAACCTGAACTCGTTTCGCTTCCTGGAAGAAGCGATCCAGTACGAGGTG
CGGCGTCAGATCGAACTGATCGAAGACGGCGGCACCGTGGTGCAGGAAACGCGTCTGTACGATCCAGACAAGCGCGAAAC
GCGTTCGATGCGCAGCAAGGAAGACGCCCACGATTACCGCTATTTCCCCGATCCCGATCTGATGCCCCTCGTGATCGACG
CATCGTGGGTCGAGCGGGTGAAGGGCGAGCTGCCGGAACTGCCCGCCGCGATGCAGAAGCGCTTCGTCGATCAATATGGC
CTCACGCCATACGATGCGGGCGTCGTCACGGCGGGCAAGGCAATGGCCGCGTACTACGAAGCCGTCGTGGCGAAGGTCGG
CGCGGCGCAGGCGAAGGTCGCGGCGAACTGGCTGATGGGCGAAGTCTCGTCGCAGTTGAACCGCGAAGGCCTCGACATTG
CCGACAGCCCGGTATCGGCTGCGCAACTCGCCCTCGTTCTGCAGCGTATCGCCGACGGCACGATCTCGAACAAGATCGCC
AAGGAAATTTTCCTCGCCATCTGGGAGGAGAAGGCCACCGACGAAGCCGCCGCCGACCGCATCATCGAAGCGAAGGGTCT
TAAGCAGATCTCCGATACAGGCGCGCTCGAAGCGATCATCGACGAAGTGCTCGCCGCGAACCAGAAGTCGGTCGAGGAAT
TCCGCGCCGGCAAGGAAAAGGCGTTCAACGCGCTGATCGGACAGGCGATGAAGGCGACCAAGGGCAAGGCGAATCCGCAG
CAGGTCAACGAACTGCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
ATCATCGGCAACTATTTCAACGAAGCACGCATGCTGCAAGTGGCCGACGCGTTCCAGCGCGCCACCGACTGGCATCGCAT
GGCGCCGGCGGGAGTCTGAT

Downstream 100 bases:

>100_bases
GTGTCGTAGCGGTATGGGTTAGGCGCGCGACATGCTGCGTGCCAGGCTGTGACGGGCTGTTGTCGCATCGAAAGTCGCGC
AACGGCCCGTTTGCTTTTTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MATQWEVVIGLETHAQLSTVSKIFSGAATQFGAAPNTQACPVDLALPGVLPVMNKGAVERAIQFGLAIDATIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKEAYEKTINLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRAEIKNLNSFRFLEEAIQYEV
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDASWVERVKGELPELPAAMQKRFVDQYG
LTPYDAGVVTAGKAMAAYYEAVVAKVGAAQAKVAANWLMGEVSSQLNREGLDIADSPVSAAQLALVLQRIADGTISNKIA
KEIFLAIWEEKATDEAAADRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG

Sequences:

>Translated_491_residues
MATQWEVVIGLETHAQLSTVSKIFSGAATQFGAAPNTQACPVDLALPGVLPVMNKGAVERAIQFGLAIDATIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKEAYEKTINLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRAEIKNLNSFRFLEEAIQYEV
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDASWVERVKGELPELPAAMQKRFVDQYG
LTPYDAGVVTAGKAMAAYYEAVVAKVGAAQAKVAANWLMGEVSSQLNREGLDIADSPVSAAQLALVLQRIADGTISNKIA
KEIFLAIWEEKATDEAAADRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG
>Mature_490_residues
ATQWEVVIGLETHAQLSTVSKIFSGAATQFGAAPNTQACPVDLALPGVLPVMNKGAVERAIQFGLAIDATIAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKEAYEKTINLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRAEIKNLNSFRFLEEAIQYEVR
RQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDASWVERVKGELPELPAAMQKRFVDQYGL
TPYDAGVVTAGKAMAAYYEAVVAKVGAAQAKVAANWLMGEVSSQLNREGLDIADSPVSAAQLALVLQRIADGTISNKIAK
EIFLAIWEEKATDEAAADRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPQQ
VNELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=38, Blast_Score=348, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=473, Percent_Identity=31.7124735729387, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=30.5927342256214, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=34.8088531187123, Blast_Score=321, Evalue=8e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53528; Mature: 53396

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQWEVVIGLETHAQLSTVSKIFSGAATQFGAAPNTQACPVDLALPGVLPVMNKGAVER
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIQFGLAIDATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKE
HHHHCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCHHH
AYEKTINLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRAEIKNLNSFRFLEEAIQYEV
HHHHHHHEEEECCCCCCCCCEEECCCCEEEECCCHHCCCHHHHHCCHHHHHHHHHHHHHH
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDASWVERV
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHH
KGELPELPAAMQKRFVDQYGLTPYDAGVVTAGKAMAAYYEAVVAKVGAAQAKVAANWLMG
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSSQLNREGLDIADSPVSAAQLALVLQRIADGTISNKIAKEIFLAIWEEKATDEAAADR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
IIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC
>Mature Secondary Structure 
ATQWEVVIGLETHAQLSTVSKIFSGAATQFGAAPNTQACPVDLALPGVLPVMNKGAVER
CCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIQFGLAIDATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKE
HHHHCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCHHH
AYEKTINLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHGLVVWLGICDGNMQEGSFRCDANVSVRPVGQKEFGTRAEIKNLNSFRFLEEAIQYEV
HHHHHHHEEEECCCCCCCCCEEECCCCEEEECCCHHCCCHHHHHCCHHHHHHHHHHHHHH
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDASWVERV
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHH
KGELPELPAAMQKRFVDQYGLTPYDAGVVTAGKAMAAYYEAVVAKVGAAQAKVAANWLMG
HCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVSSQLNREGLDIADSPVSAAQLALVLQRIADGTISNKIAKEIFLAIWEEKATDEAAADR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
IIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA