Definition Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' chromosome chromosome I, complete sequence.
Accession NC_010602
Length 3,599,677

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The map label for this gene is serS

Identifier: 183222806

GI number: 183222806

Start: 3586228

End: 3587484

Strand: Reverse

Name: serS

Synonym: LEPBI_I3467

Alternate gene names: 183222806

Gene position: 3587484-3586228 (Counterclockwise)

Preceding gene: 183222807

Following gene: 183222805

Centisome position: 99.66

GC content: 40.57

Gene sequence:

>1257_bases
ATGCTTGATATCAATCGAATTGTTCAGAATCCAGAAGAGTTACTTTCCACCTTACAAAAACGTGGTGTAGTTTCTTCTGA
CATAGAAGCAAAGATCAAATCTGTTTCCGAAAAACAAAGAATACTCAAATTGGAAGTAGAGGAACTGCGTGCTGAACGAA
ACCGAGTCTCCAAAGAAATTGGAATCCAAAAATCGCAAGGAAAGGACATCACTGAAATTTCCAATTCGATGAAAGGTGTT
GGCGACAGGATCAAATCCATTGAAGAAGAACTAACGAAAGAAGAAGAATCCTTACACGAGTTGAATTTGGGTTTACCAAA
CTTACTTGATCCATCTGTACCGGAGGGAAAATCGGAAGAGGACAATGTCCTCATCCGGCAATGGGGTGAAATTCCAAAAC
TTTCTTTCGAAGCAAAAACACATTTTGATATTGGTGAAAAATTAGGAATTTTCGATTTCGAACGCGGAGTGAAACTTTCT
GGTGCAAGGTTTTACACCTATCGTGGGTTAGGTGCAAAATTAGAACGGTCATTGATGAACCTGATGCTTGATACTCATAC
TTCAGAAAATGGTTATGAAGAGATGTGGGTTCCTGTGCTTGTGAATGATGAATCCATGACTGCCACAGGTCAACTTCCCA
AATTTGCAGAAGATTTTTATCGATTGGAAAAGGACGGCCTCAATCTCATTCCCACGGCGGAAGTTCCATTAACCAATTAT
TACCGCGATGAAATCATCCAAGAGAAAGAATTACCCATTTCCGTTTGTGCTCACACCTCATGTTTTAGACGGGAAGCGGG
TTCTTATGGTAGAGACACAAGAGGTCTTGTAAGGGTCCATCAGTTTCAAAAAGTGGAACTTGTTAAATTTGTCGAACCAG
AGAAATCAGGGGAAGCACATGAAAAGATGTTACAGGATGCAGAATCCATTTTGCAAAAATTAAAACTCCCGTATCGAGTG
ATGTTACTTTGTAGTAAGGATATGTCGAGTGCCTCCTCCAAAACCTATGATATCGAGGTATGGATGCCAGGACTAGGTCG
ATTTATGGAAATTTCTTCAGTTTCTAACTTCAAAGACTATCAAGCAAGACGCGGAAAAATTCGATACAAGTCAAAGGAAG
GGAAAAACCTGCTCGTCCATACTTTGAATGGTTCTGGTCTTGCGATCGGTCGAACACTGGCAGCGGTGATCGAAAATTAC
CAATCCGAAGATGGAACCTTCCAAATTCCGGATGTACTAAAACCTTACATTCGTTAG

Upstream 100 bases:

>100_bases
TTAGAAAAAATGTTTAACCTACGCACAGAACCCAATGCGGAAGTAGCAGATCGTATTTATCAGAATTCAATAAAATTTAC
AGAAAGAAAGGCCTATCACC

Downstream 100 bases:

>100_bases
AATTTATGGGCAGATGACCGAGGTGTCAAATGCCCATGAACAATTTTCATAGAGAATTAAACTCTAATTTTCCCATTCAG
TTTCTAAATCGAATCAATTA

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEIGIQKSQGKDITEISNSMKGV
GDRIKSIEEELTKEEESLHELNLGLPNLLDPSVPEGKSEEDNVLIRQWGEIPKLSFEAKTHFDIGEKLGIFDFERGVKLS
GARFYTYRGLGAKLERSLMNLMLDTHTSENGYEEMWVPVLVNDESMTATGQLPKFAEDFYRLEKDGLNLIPTAEVPLTNY
YRDEIIQEKELPISVCAHTSCFRREAGSYGRDTRGLVRVHQFQKVELVKFVEPEKSGEAHEKMLQDAESILQKLKLPYRV
MLLCSKDMSSASSKTYDIEVWMPGLGRFMEISSVSNFKDYQARRGKIRYKSKEGKNLLVHTLNGSGLAIGRTLAAVIENY
QSEDGTFQIPDVLKPYIR

Sequences:

>Translated_418_residues
MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEIGIQKSQGKDITEISNSMKGV
GDRIKSIEEELTKEEESLHELNLGLPNLLDPSVPEGKSEEDNVLIRQWGEIPKLSFEAKTHFDIGEKLGIFDFERGVKLS
GARFYTYRGLGAKLERSLMNLMLDTHTSENGYEEMWVPVLVNDESMTATGQLPKFAEDFYRLEKDGLNLIPTAEVPLTNY
YRDEIIQEKELPISVCAHTSCFRREAGSYGRDTRGLVRVHQFQKVELVKFVEPEKSGEAHEKMLQDAESILQKLKLPYRV
MLLCSKDMSSASSKTYDIEVWMPGLGRFMEISSVSNFKDYQARRGKIRYKSKEGKNLLVHTLNGSGLAIGRTLAAVIENY
QSEDGTFQIPDVLKPYIR
>Mature_418_residues
MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEIGIQKSQGKDITEISNSMKGV
GDRIKSIEEELTKEEESLHELNLGLPNLLDPSVPEGKSEEDNVLIRQWGEIPKLSFEAKTHFDIGEKLGIFDFERGVKLS
GARFYTYRGLGAKLERSLMNLMLDTHTSENGYEEMWVPVLVNDESMTATGQLPKFAEDFYRLEKDGLNLIPTAEVPLTNY
YRDEIIQEKELPISVCAHTSCFRREAGSYGRDTRGLVRVHQFQKVELVKFVEPEKSGEAHEKMLQDAESILQKLKLPYRV
MLLCSKDMSSASSKTYDIEVWMPGLGRFMEISSVSNFKDYQARRGKIRYKSKEGKNLLVHTLNGSGLAIGRTLAAVIENY
QSEDGTFQIPDVLKPYIR

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=328, Percent_Identity=38.4146341463415, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI8923421, Length=433, Percent_Identity=32.5635103926097, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI225579074, Length=438, Percent_Identity=31.9634703196347, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI1787120, Length=425, Percent_Identity=47.2941176470588, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17539260, Length=340, Percent_Identity=35.2941176470588, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17542084, Length=329, Percent_Identity=29.483282674772, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320226, Length=438, Percent_Identity=34.703196347032, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321799, Length=428, Percent_Identity=32.0093457943925, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24581334, Length=387, Percent_Identity=32.5581395348837, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI17737985, Length=277, Percent_Identity=32.4909747292419, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_LEPBA (B0S8Z4)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964393.1
- ProteinModelPortal:   B0S8Z4
- SMR:   B0S8Z4
- GeneID:   6389054
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3352
- HOGENOM:   HBG629391
- OMA:   PKFADDM
- ProtClustDB:   PRK05431
- BioCyc:   LBIF355278:LBF_3352-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 47585; Mature: 47585

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 279-279 BINDING 286-286 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEI
CCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIQKSQGKDITEISNSMKGVGDRIKSIEEELTKEEESLHELNLGLPNLLDPSVPEGKSEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DNVLIRQWGEIPKLSFEAKTHFDIGEKLGIFDFERGVKLSGARFYTYRGLGAKLERSLMN
CCCHHHHCCCCCCEEECCCCCCCCCCCCCCEECCCCCEECCCEEEEECCCCHHHHHHHHH
LMLDTHTSENGYEEMWVPVLVNDESMTATGQLPKFAEDFYRLEKDGLNLIPTAEVPLTNY
HHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
YRDEIIQEKELPISVCAHTSCFRREAGSYGRDTRGLVRVHQFQKVELVKFVEPEKSGEAH
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
EKMLQDAESILQKLKLPYRVMLLCSKDMSSASSKTYDIEVWMPGLGRFMEISSVSNFKDY
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHEEHHHCCCHHHH
QARRGKIRYKSKEGKNLLVHTLNGSGLAIGRTLAAVIENYQSEDGTFQIPDVLKPYIR
HHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHCC
>Mature Secondary Structure
MLDINRIVQNPEELLSTLQKRGVVSSDIEAKIKSVSEKQRILKLEVEELRAERNRVSKEI
CCCHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIQKSQGKDITEISNSMKGVGDRIKSIEEELTKEEESLHELNLGLPNLLDPSVPEGKSEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DNVLIRQWGEIPKLSFEAKTHFDIGEKLGIFDFERGVKLSGARFYTYRGLGAKLERSLMN
CCCHHHHCCCCCCEEECCCCCCCCCCCCCCEECCCCCEECCCEEEEECCCCHHHHHHHHH
LMLDTHTSENGYEEMWVPVLVNDESMTATGQLPKFAEDFYRLEKDGLNLIPTAEVPLTNY
HHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHH
YRDEIIQEKELPISVCAHTSCFRREAGSYGRDTRGLVRVHQFQKVELVKFVEPEKSGEAH
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
EKMLQDAESILQKLKLPYRVMLLCSKDMSSASSKTYDIEVWMPGLGRFMEISSVSNFKDY
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHEEHHHCCCHHHH
QARRGKIRYKSKEGKNLLVHTLNGSGLAIGRTLAAVIENYQSEDGTFQIPDVLKPYIR
HHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA