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The map label for this gene is nemA [H]

Identifier: 183222690

GI number: 183222690

Start: 3452739

End: 3453815

Strand: Reverse

Name: nemA [H]

Synonym: LEPBI_I3346

Alternate gene names: 183222690

Gene position: 3453815-3452739 (Counterclockwise)

Preceding gene: 183222691

Following gene: 183222689

Centisome position: 95.95

GC content: 38.63

Gene sequence:

>1077_bases
ATGAATTCGTTATACCAAAAAACAAAATTAGGCAATTTGGAGTTAAAAAACCGTGTTGTCATGGCACCGATGACTCGATC
AAGATCGATCAACAATATCCCTGGTGATCTTGTTGCAACATATTATGAACAAAGGTCTGAAGCAGGACTCATCATAACGG
AAGGAACATCTCCATCACCGAATGGTCTTGGTTATGCGAGAATACCTGGAATTTTTTCAAAGGAACAAACTGCCGGATGG
AAAAAAGTAACAGAAAAAGTTCATAACAAAGGAAGTAAAATTTTTGTTCAATTGATGCACACAGGTCGCATCGGCCACGA
ACTCAATTTACCAAAATCGGCAAAAGTAATGGGACCATCTGCTATTATGGCAAAGGGAAAAATGTGGACTGATAGTGATG
GAATGTTAGACCACCCAACTCCACTGGAAATGTCCAAGGAAGAGATTTTATCAACAATTGAAGAATTTGTTGATGCTTCC
AAAAATGCGATCGCCGCAGGTTTTGATGGTGTAGAACTTCATGCAGCAAACGGATATTTGTTGGAACAATTTTTACATCC
TTCTGCAAACCAAAGAACAGATGAATACGGTGGTTCCATTGAAAACCGAATTCGTTTTATCTTAGAGGTTTCTAAGGCAG
TGAGTGAAGCCATCGGAAAGGAAAAAACGGGAATTCGGTTATCTCCTTATGGCGCGTTCAATGACTTATTCCCTTTCCCT
GAAACACATGATGAATATTCATTGTTAGCTGAAAAATTGAATCAAATCGGAATTGTCTACATTCATTTAGTTGATCACTC
ATCGATGGGAGCACCAATTGTAGAACCAAAAACTGTCTCTGCCATTCGAAACAATTTTAAAGGCACTCTCATTTTAAGTG
GTGGTTATGATGCGATACGTGCAGAAAAGGATTTAACTTCGGGTAACGCAGATTTGGTGGCATTTGGTAAACCATTTTTG
GCAAATCCAGATCTAGTCACAAAATTCCAAAAAAATATTCCTCTCGCCAATTTTGACGAATCAACTTTGTATACACCCGG
TGAAAAAGGTTACACAGACTATCCATTTTCATCCTGA

Upstream 100 bases:

>100_bases
CTTCTATTTTTTTGGAAAAAATACTTTGATATTGAATTATTTTATATCAAAATAATATCCACCTTTGTCTATGTTCTATA
AGGACTTTTAAGGAAATCAA

Downstream 100 bases:

>100_bases
CCCAATGGAAAAAGCGCTCAAGCATTTGGGCGCCTCCAAATCAATCTATAACATAAGTTAATTGAATGAAAGGAGCGGGC
TCCTCCGGGGTTCGCATTCG

Product: putative NADPH dehydrogenase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNSLYQKTKLGNLELKNRVVMAPMTRSRSINNIPGDLVATYYEQRSEAGLIITEGTSPSPNGLGYARIPGIFSKEQTAGW
KKVTEKVHNKGSKIFVQLMHTGRIGHELNLPKSAKVMGPSAIMAKGKMWTDSDGMLDHPTPLEMSKEEILSTIEEFVDAS
KNAIAAGFDGVELHAANGYLLEQFLHPSANQRTDEYGGSIENRIRFILEVSKAVSEAIGKEKTGIRLSPYGAFNDLFPFP
ETHDEYSLLAEKLNQIGIVYIHLVDHSSMGAPIVEPKTVSAIRNNFKGTLILSGGYDAIRAEKDLTSGNADLVAFGKPFL
ANPDLVTKFQKNIPLANFDESTLYTPGEKGYTDYPFSS

Sequences:

>Translated_358_residues
MNSLYQKTKLGNLELKNRVVMAPMTRSRSINNIPGDLVATYYEQRSEAGLIITEGTSPSPNGLGYARIPGIFSKEQTAGW
KKVTEKVHNKGSKIFVQLMHTGRIGHELNLPKSAKVMGPSAIMAKGKMWTDSDGMLDHPTPLEMSKEEILSTIEEFVDAS
KNAIAAGFDGVELHAANGYLLEQFLHPSANQRTDEYGGSIENRIRFILEVSKAVSEAIGKEKTGIRLSPYGAFNDLFPFP
ETHDEYSLLAEKLNQIGIVYIHLVDHSSMGAPIVEPKTVSAIRNNFKGTLILSGGYDAIRAEKDLTSGNADLVAFGKPFL
ANPDLVTKFQKNIPLANFDESTLYTPGEKGYTDYPFSS
>Mature_358_residues
MNSLYQKTKLGNLELKNRVVMAPMTRSRSINNIPGDLVATYYEQRSEAGLIITEGTSPSPNGLGYARIPGIFSKEQTAGW
KKVTEKVHNKGSKIFVQLMHTGRIGHELNLPKSAKVMGPSAIMAKGKMWTDSDGMLDHPTPLEMSKEEILSTIEEFVDAS
KNAIAAGFDGVELHAANGYLLEQFLHPSANQRTDEYGGSIENRIRFILEVSKAVSEAIGKEKTGIRLSPYGAFNDLFPFP
ETHDEYSLLAEKLNQIGIVYIHLVDHSSMGAPIVEPKTVSAIRNNFKGTLILSGGYDAIRAEKDLTSGNADLVAFGKPFL
ANPDLVTKFQKNIPLANFDESTLYTPGEKGYTDYPFSS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=358, Percent_Identity=42.4581005586592, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789463, Length=343, Percent_Identity=31.7784256559767, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17565138, Length=361, Percent_Identity=28.808864265928, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=351, Percent_Identity=30.1994301994302, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=26.8571428571429, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=347, Percent_Identity=26.8011527377522, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=355, Percent_Identity=28.169014084507, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=350, Percent_Identity=25.4285714285714, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=225, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=39.7905759162304, Blast_Score=235, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=376, Percent_Identity=38.563829787234, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39305; Mature: 39305

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLYQKTKLGNLELKNRVVMAPMTRSRSINNIPGDLVATYYEQRSEAGLIITEGTSPSP
CCCHHHHHCCCCEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
NGLGYARIPGIFSKEQTAGWKKVTEKVHNKGSKIFVQLMHTGRIGHELNLPKSAKVMGPS
CCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCH
AIMAKGKMWTDSDGMLDHPTPLEMSKEEILSTIEEFVDASKNAIAAGFDGVELHAANGYL
HHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHH
LEQFLHPSANQRTDEYGGSIENRIRFILEVSKAVSEAIGKEKTGIRLSPYGAFNDLFPFP
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
ETHDEYSLLAEKLNQIGIVYIHLVDHSSMGAPIVEPKTVSAIRNNFKGTLILSGGYDAIR
CCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHH
AEKDLTSGNADLVAFGKPFLANPDLVTKFQKNIPLANFDESTLYTPGEKGYTDYPFSS
CCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNSLYQKTKLGNLELKNRVVMAPMTRSRSINNIPGDLVATYYEQRSEAGLIITEGTSPSP
CCCHHHHHCCCCEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
NGLGYARIPGIFSKEQTAGWKKVTEKVHNKGSKIFVQLMHTGRIGHELNLPKSAKVMGPS
CCCCCHHCCCCCCCHHHCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCH
AIMAKGKMWTDSDGMLDHPTPLEMSKEEILSTIEEFVDASKNAIAAGFDGVELHAANGYL
HHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHH
LEQFLHPSANQRTDEYGGSIENRIRFILEVSKAVSEAIGKEKTGIRLSPYGAFNDLFPFP
HHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
ETHDEYSLLAEKLNQIGIVYIHLVDHSSMGAPIVEPKTVSAIRNNFKGTLILSGGYDAIR
CCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHH
AEKDLTSGNADLVAFGKPFLANPDLVTKFQKNIPLANFDESTLYTPGEKGYTDYPFSS
CCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]