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The map label for this gene is czcA [H]

Identifier: 183222485

GI number: 183222485

Start: 3251906

End: 3252397

Strand: Reverse

Name: czcA [H]

Synonym: LEPBI_I3140

Alternate gene names: 183222485

Gene position: 3252397-3251906 (Counterclockwise)

Preceding gene: 183222486

Following gene: 183222484

Centisome position: 90.35

GC content: 41.26

Gene sequence:

>492_bases
ATGTTATTGGAGCACGAAACTGAAAATTTGATGAAAGCCAATCCTTATGAAAGATACGAAAGTCGCGATATACATAGTTA
TAGACAAGCTATACTCGTTTACCTAAGTTTACCATTTGCAACTATCGGAGGAGTATTTGCGCTTTATTTTCGAGGCATGA
ATTTCAGTATTTCGGCGGCAATTGGTTTCATAGCCCTCTCTGGGATTGCCATCCTCAATGGTGTAGTCAAAATTCACAAT
ATCAACTCTCTTCGAAATTCAGGAATGTCTATCAGGGAAGCCGTACAAGAAGCGGCTGTATCTAGACTACGTCCTGTTCT
TATGACTGCATTGGTTGCTTCATTGGGTTTTTTACCAATGGCAATCGGAGAAGGCTTGGGCGCAGAAGTACAAAGGCCTC
TAGCGACAGTCGTGATTGGTGGACTCATATCGTCCACATTGCTTACGTTATTTTTACTTCCTGTATTTTATGAGTGGTTG
GAGGAGAAATGA

Upstream 100 bases:

>100_bases
CAAGAAAAAGGAGACGAGAAAATAAATTCCGTGTAAGAGGAGAATATACAAATTGAACTCAGTGGATTAGTAGGGGGAAA
CAGTTGAAAAATCTCTATAG

Downstream 100 bases:

>100_bases
AATGTTATAAAAAATCAGGTTTTGACAAAGATTGTTTTAAAATAAAAAAAGTATAAATTCAAATATTGATAAAAAAGGAT
AAAATAAGATGAATTTCAAA

Product: putative transporter

Products: Proton [Cytoplasm]; silver [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MLLEHETENLMKANPYERYESRDIHSYRQAILVYLSLPFATIGGVFALYFRGMNFSISAAIGFIALSGIAILNGVVKIHN
INSLRNSGMSIREAVQEAAVSRLRPVLMTALVASLGFLPMAIGEGLGAEVQRPLATVVIGGLISSTLLTLFLLPVFYEWL
EEK

Sequences:

>Translated_163_residues
MLLEHETENLMKANPYERYESRDIHSYRQAILVYLSLPFATIGGVFALYFRGMNFSISAAIGFIALSGIAILNGVVKIHN
INSLRNSGMSIREAVQEAAVSRLRPVLMTALVASLGFLPMAIGEGLGAEVQRPLATVVIGGLISSTLLTLFLLPVFYEWL
EEK
>Mature_163_residues
MLLEHETENLMKANPYERYESRDIHSYRQAILVYLSLPFATIGGVFALYFRGMNFSISAAIGFIALSGIAILNGVVKIHN
INSLRNSGMSIREAVQEAAVSRLRPVLMTALVASLGFLPMAIGEGLGAEVQRPLATVVIGGLISSTLLTLFLLPVFYEWL
EEK

Specific function: Has a low cation transport activity for cobalt, it is essential for the expression of cobalt, zinc, and cadmium resistance. CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete CZC efflux system (CzcABC) [H]

COG id: COG3696

COG function: function code P; Putative silver efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1786788, Length=145, Percent_Identity=39.3103448275862, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1788391, Length=139, Percent_Identity=33.8129496402878, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788814, Length=133, Percent_Identity=32.3308270676692, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788390, Length=134, Percent_Identity=42.5373134328358, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789930, Length=120, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786667, Length=119, Percent_Identity=31.9327731092437, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004763 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 17887; Mature: 17887

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEHETENLMKANPYERYESRDIHSYRQAILVYLSLPFATIGGVFALYFRGMNFSISAA
CCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IGFIALSGIAILNGVVKIHNINSLRNSGMSIREAVQEAAVSRLRPVLMTALVASLGFLPM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGEGLGAEVQRPLATVVIGGLISSTLLTLFLLPVFYEWLEEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLEHETENLMKANPYERYESRDIHSYRQAILVYLSLPFATIGGVFALYFRGMNFSISAA
CCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IGFIALSGIAILNGVVKIHNINSLRNSGMSIREAVQEAAVSRLRPVLMTALVASLGFLPM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIGEGLGAEVQRPLATVVIGGLISSTLLTLFLLPVFYEWLEEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; silver [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + silver [Cytoplasm] = Proton [Cytoplasm] + silver [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]