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The map label for this gene is desA3 [H]

Identifier: 183222450

GI number: 183222450

Start: 3220359

End: 3221540

Strand: Reverse

Name: desA3 [H]

Synonym: LEPBI_I3104

Alternate gene names: 183222450

Gene position: 3221540-3220359 (Counterclockwise)

Preceding gene: 183222451

Following gene: 183222448

Centisome position: 89.5

GC content: 35.03

Gene sequence:

>1182_bases
ATGAGAACAATTAGTAAAAAATTAAACAAAGAAGAAATTGAAGCATTCGGAAAAGAAGTTGATACACTTCGTGAAGAAGT
AATGGCGAAAGTCGGAAAAGAAGATGCTGATCATATTCGTTTTATCTATAAAACCTATCGTTATACAGAAGTTTTGGGAA
GAGGTTTGATCCACTTTAGTTTTGAACCAATTTCCTTCGTTGCTGGAACGCTTTTGTTATCTATTTCCAAAATCATAAAT
AATATGGAACTAGGTCATAACGTCTTACATGGTCAATATGATTGGATGAATGACCCGCGTTTCAACTCTAGAACATTTGA
ATGGGACATTGTTTGTAATGCCCACCAATGGAAGTTTTACCATAATTATATGCATCATACATATACAAATGTTTTGAACA
AAGACCATGACTATGGATATAATTTCACAAGATTAACAGAAGCACAGAAGTGGAAACCAGTTCACCTAACACAGCCTTTT
ACGAATATGTTTTTGGCGATGAACTTTCAATGGGGAATTGGTGCCCATGGATATCGAGTTGAGTATTTAGAAACTCCCAA
AAAACTAAGAAAGAAAAAAACACTTAATGATTACAAAGCACTATTCTTTAAAAAAATTGAACTCCAACTCCTAAAAGATT
ATATTTTGTTTCCGGCTCTTGCTGGTCTCAATTTTCCAAAGGTAATTTTAGGAAATTTATTGGCCAATTTGATCAGAAAT
CTTTGGACATATGCTGTAATCTTCTGCGGTCATTTTACAGAAAATGCAGAGTCATTCACAACAGATGAAATTGTCGGTGA
AACAAAAGCACAATGGTATTTAAGACAACTCAAAGGATCTTCGAATTTGGAAGGTAACAATTTGTTTTACACGATGACAG
GTCATTTGAGCCATCAAATCGAACATCATATGTTCCCAGACATGCCTGCGAAACGATACCGTGAAGTAGCTCCACGTTTG
AAGGAAATCTGTGCAAAGTATGGGCAACATTACAATACCGGAAGTTTCGTAAAACAATTTGCATCTGTATGGAAACGAAT
TATCGCGTATTCATTTCCAGATCATATTGCATCGAAGGTTATGGGAAAACGTAAAAAGTTTTTAGAACCAACGATTGTAT
TAAGCCAACCAAGCTTCCAAGTATCTCTTCCATCTGAAGAGAAATCTGTAACACTCACTTAA

Upstream 100 bases:

>100_bases
GAAAATATCCAAATCTGCGTTTCTAGAGCTGAATCCAATTTGGAATTAGAACTATAACTTTTAATCACAAAGGAAATTGA
AATTATCATAGGAAAACAAA

Downstream 100 bases:

>100_bases
TCTGAACGATCTCGGACTTCCTTTACCTCGCAATGGAGGAAGTCCTTCCCTTTACTTTCTCGTGGGTCCTCTTTACGGAG
GACCCTTTTTTTTTATAAGG

Product: putative fatty acid desaturase

Products: NA

Alternate protein names: Delta(9)-desaturase; Stearoyl-CoA desaturase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRTISKKLNKEEIEAFGKEVDTLREEVMAKVGKEDADHIRFIYKTYRYTEVLGRGLIHFSFEPISFVAGTLLLSISKIIN
NMELGHNVLHGQYDWMNDPRFNSRTFEWDIVCNAHQWKFYHNYMHHTYTNVLNKDHDYGYNFTRLTEAQKWKPVHLTQPF
TNMFLAMNFQWGIGAHGYRVEYLETPKKLRKKKTLNDYKALFFKKIELQLLKDYILFPALAGLNFPKVILGNLLANLIRN
LWTYAVIFCGHFTENAESFTTDEIVGETKAQWYLRQLKGSSNLEGNNLFYTMTGHLSHQIEHHMFPDMPAKRYREVAPRL
KEICAKYGQHYNTGSFVKQFASVWKRIIAYSFPDHIASKVMGKRKKFLEPTIVLSQPSFQVSLPSEEKSVTLT

Sequences:

>Translated_393_residues
MRTISKKLNKEEIEAFGKEVDTLREEVMAKVGKEDADHIRFIYKTYRYTEVLGRGLIHFSFEPISFVAGTLLLSISKIIN
NMELGHNVLHGQYDWMNDPRFNSRTFEWDIVCNAHQWKFYHNYMHHTYTNVLNKDHDYGYNFTRLTEAQKWKPVHLTQPF
TNMFLAMNFQWGIGAHGYRVEYLETPKKLRKKKTLNDYKALFFKKIELQLLKDYILFPALAGLNFPKVILGNLLANLIRN
LWTYAVIFCGHFTENAESFTTDEIVGETKAQWYLRQLKGSSNLEGNNLFYTMTGHLSHQIEHHMFPDMPAKRYREVAPRL
KEICAKYGQHYNTGSFVKQFASVWKRIIAYSFPDHIASKVMGKRKKFLEPTIVLSQPSFQVSLPSEEKSVTLT
>Mature_393_residues
MRTISKKLNKEEIEAFGKEVDTLREEVMAKVGKEDADHIRFIYKTYRYTEVLGRGLIHFSFEPISFVAGTLLLSISKIIN
NMELGHNVLHGQYDWMNDPRFNSRTFEWDIVCNAHQWKFYHNYMHHTYTNVLNKDHDYGYNFTRLTEAQKWKPVHLTQPF
TNMFLAMNFQWGIGAHGYRVEYLETPKKLRKKKTLNDYKALFFKKIELQLLKDYILFPALAGLNFPKVILGNLLANLIRN
LWTYAVIFCGHFTENAESFTTDEIVGETKAQWYLRQLKGSSNLEGNNLFYTMTGHLSHQIEHHMFPDMPAKRYREVAPRL
KEICAKYGQHYNTGSFVKQFASVWKRIIAYSFPDHIASKVMGKRKKFLEPTIVLSQPSFQVSLPSEEKSVTLT

Specific function: Desaturates stearoyl-CoA to form oleoyl-CoA [H]

COG id: COG3239

COG function: function code I; Fatty acid desaturase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable). Note=Has been shown to be membrane-associated but no reliable trans-membrane regions are predicted by bioinformatics tools [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fatty acid desaturase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005804 [H]

Pfam domain/function: PF00487 FA_desaturase [H]

EC number: NA

Molecular weight: Translated: 46094; Mature: 46094

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTISKKLNKEEIEAFGKEVDTLREEVMAKVGKEDADHIRFIYKTYRYTEVLGRGLIHFS
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
FEPISFVAGTLLLSISKIINNMELGHNVLHGQYDWMNDPRFNSRTFEWDIVCNAHQWKFY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHH
HNYMHHTYTNVLNKDHDYGYNFTRLTEAQKWKPVHLTQPFTNMFLAMNFQWGIGAHGYRV
HHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCEECCCHHHHEEEEEEECCCCCCCCEEE
EYLETPKKLRKKKTLNDYKALFFKKIELQLLKDYILFPALAGLNFPKVILGNLLANLIRN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LWTYAVIFCGHFTENAESFTTDEIVGETKAQWYLRQLKGSSNLEGNNLFYTMTGHLSHQI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHH
EHHMFPDMPAKRYREVAPRLKEICAKYGQHYNTGSFVKQFASVWKRIIAYSFPDHIASKV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
MGKRKKFLEPTIVLSQPSFQVSLPSEEKSVTLT
HHHHHHHCCCEEEEECCCEEEECCCCCCCEECC
>Mature Secondary Structure
MRTISKKLNKEEIEAFGKEVDTLREEVMAKVGKEDADHIRFIYKTYRYTEVLGRGLIHFS
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
FEPISFVAGTLLLSISKIINNMELGHNVLHGQYDWMNDPRFNSRTFEWDIVCNAHQWKFY
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHH
HNYMHHTYTNVLNKDHDYGYNFTRLTEAQKWKPVHLTQPFTNMFLAMNFQWGIGAHGYRV
HHHHHHHHHHHHCCCCCCCCCHHEECCCCCCCCEECCCHHHHEEEEEEECCCCCCCCEEE
EYLETPKKLRKKKTLNDYKALFFKKIELQLLKDYILFPALAGLNFPKVILGNLLANLIRN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LWTYAVIFCGHFTENAESFTTDEIVGETKAQWYLRQLKGSSNLEGNNLFYTMTGHLSHQI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHH
EHHMFPDMPAKRYREVAPRLKEICAKYGQHYNTGSFVKQFASVWKRIIAYSFPDHIASKV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
MGKRKKFLEPTIVLSQPSFQVSLPSEEKSVTLT
HHHHHHHCCCEEEEECCCEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]