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The map label for this gene is cobS

Identifier: 183222287

GI number: 183222287

Start: 3038134

End: 3038961

Strand: Direct

Name: cobS

Synonym: LEPBI_I2939

Alternate gene names: 183222287

Gene position: 3038134-3038961 (Clockwise)

Preceding gene: 183222286

Following gene: 183222288

Centisome position: 84.4

GC content: 38.16

Gene sequence:

>828_bases
ATGAAATATATCCTATTAGAAATCCGTTTGTTTTTTGTTTGTATGTCCTTTCTCACAAGGATTCCTTCTCCGCGTTGGAT
CGGTTTTCAAGAAGAGTGGTTACACCATTCCATTAAATATTCTCCCTCTGTTGGTTTTTTACTCGGCAGCTTACAATGGT
TTGTTTTCCTTCTGTTCCAATTTTTATTTGGACCTACGATTGCGTTTACAATTTCTCTTGGTTTTTTACTCATTCTCACT
GGTGCCTTTCACGAAGATGGATTTTCCGATTTTTGTGATGGGATTGGTGGCGGATGGAAACGAGAAGATATTTTACGCAT
TATGAAAGACAGTCGTGTCGGAAGTTTCGGTGCCGCCGGTATTTCGTTACTTTTACTTTTAAAAGTCTTAGGTGTCTCAG
AAACCTTTCACCAATATGAAATCAAAGGACTTCCATTTACCTTTGGTTCGTCTGCCCAAATTCACCTCCTCAGTGTTTGG
TTGTACTTTGTGACTGCCCAGAGCTTTAGTCGATTTTTATCAATACTCATGATGAAACTTTTACCTTATGCGAAAGAAGA
AGGATATGCAAAACCGATGGCAAAAGAAATCAATTGGCCTCAAATTTATTTTGCCTGTATCTTTGGAGTTACTCCTTTTT
TAGCACTGGTGTATTTACATCCTTACTTTTTGCTAAGCCTACTATGTATCATCCCAAGTTTTGTTTATATGTTTTCGCTC
ATGAAACGATGGATCCAAGGATTCACTGGTGATTGTTTGGGGGCTGTCCAACAAGTTGTGGAAACTTGCATTTGGATCTC
TGGAGTATTTGTATGGATCTCTATTTAA

Upstream 100 bases:

>100_bases
CGCCTACCCTCTCATCGAACTCAGTGTAAAATTTTTGAATGAAATGGCTTCCTTTGCCGACGCAGGTGTCAGCGACTCAG
AGACAAAATCTTAATTCACT

Downstream 100 bases:

>100_bases
TTCGCCATCCAGAAACCATAGCACCCAAAGGCACTTGTTACGGGCGAACTGATTTTCCACTCAAATACCCTGTAGAAGAC
ACTGCAGAAACTACATTCGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKYILLEIRLFFVCMSFLTRIPSPRWIGFQEEWLHHSIKYSPSVGFLLGSLQWFVFLLFQFLFGPTIAFTISLGFLLILT
GAFHEDGFSDFCDGIGGGWKREDILRIMKDSRVGSFGAAGISLLLLLKVLGVSETFHQYEIKGLPFTFGSSAQIHLLSVW
LYFVTAQSFSRFLSILMMKLLPYAKEEGYAKPMAKEINWPQIYFACIFGVTPFLALVYLHPYFLLSLLCIIPSFVYMFSL
MKRWIQGFTGDCLGAVQQVVETCIWISGVFVWISI

Sequences:

>Translated_275_residues
MKYILLEIRLFFVCMSFLTRIPSPRWIGFQEEWLHHSIKYSPSVGFLLGSLQWFVFLLFQFLFGPTIAFTISLGFLLILT
GAFHEDGFSDFCDGIGGGWKREDILRIMKDSRVGSFGAAGISLLLLLKVLGVSETFHQYEIKGLPFTFGSSAQIHLLSVW
LYFVTAQSFSRFLSILMMKLLPYAKEEGYAKPMAKEINWPQIYFACIFGVTPFLALVYLHPYFLLSLLCIIPSFVYMFSL
MKRWIQGFTGDCLGAVQQVVETCIWISGVFVWISI
>Mature_275_residues
MKYILLEIRLFFVCMSFLTRIPSPRWIGFQEEWLHHSIKYSPSVGFLLGSLQWFVFLLFQFLFGPTIAFTISLGFLLILT
GAFHEDGFSDFCDGIGGGWKREDILRIMKDSRVGSFGAAGISLLLLLKVLGVSETFHQYEIKGLPFTFGSSAQIHLLSVW
LYFVTAQSFSRFLSILMMKLLPYAKEEGYAKPMAKEINWPQIYFACIFGVTPFLALVYLHPYFLLSLLCIIPSFVYMFSL
MKRWIQGFTGDCLGAVQQVVETCIWISGVFVWISI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=257, Percent_Identity=30.7392996108949, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_LEPBA (B0SFE6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963892.1
- GeneID:   6388957
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2839
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   CLSK2337041
- BioCyc:   LBIF355278:LBF_2839-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 31474; Mature: 31474

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b91438c)-; HASH(0x1db0802c)-; HASH(0x1dac2da0)-; HASH(0x1d51b730)-; HASH(0x1daed29c)-; HASH(0x1db02828)-;

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEH
>Mature Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA