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The map label for this gene is thiE

Identifier: 183222073

GI number: 183222073

Start: 2810312

End: 2810944

Strand: Direct

Name: thiE

Synonym: LEPBI_I2715

Alternate gene names: 183222073

Gene position: 2810312-2810944 (Clockwise)

Preceding gene: 183222072

Following gene: 183222075

Centisome position: 78.07

GC content: 46.76

Gene sequence:

>633_bases
ATGCAAAATAAAATCCAAGGGGTGTATCTTGTCACAGATAGACCCCTTTGCCTCCACCATAAACTGGAAGAAGTGGTGCA
AATGGCCGCTTCTGGTGGAGTTTCCCTTGTCCAACTCAGGGAAAAGGATTCCACCTCTCGTGAATTTTTAGAACTTGCCA
TCCACCTAAAATTCATCCTAAGCCCCTTCCAAGTCCCCCTCCTCATCAATGACCGAGTGGATCTGTGTTTGGCATCAGGT
GCCGATGGGGTCCACCTAGGGCAAACAGACCTTCCTTGGTTAGAAGCAAGGCGGATCCTCGGAAAGGACGCCATCATTGG
ACTCTCCATTGAAACAAAAGAAGACTTTGCCACACTGACAAAAGAAGATCCAAATCCTCAATTGGAGTATTTGGCAGTAT
CCCCTGTGTTTGATACACCCACTAAAACCAATACCAAAGAAGCACTAGGACTAGCAGGCGTTCGCTGGCTAAAAGAAAAA
ACAGACATTCCCGTTGTGGCCATAGGTGGCATCAACATTTCCAATGCAAAGGATGTGATTGGCGCGGGGGCCGATATGAT
TGCCGTTGTCAGTGCGATTTGTTCAGCTAAAAACCCAAAAGAAGCGACTGTAGCCTTACGAAATCAATTCTAA

Upstream 100 bases:

>100_bases
GAAATGGCAAAATCCAAAACGGCAAGTCCTGGCAGTTTCCAAGTGGCATTCCTTGATGCCCTGTATGAACTGAATGCAGA
TACCATTAAACAAAAGTTAA

Downstream 100 bases:

>100_bases
GAATTTTTTTTTGTCACTTGGTTTCGGTTCCAAATGGAGACACCGATCCGTTCGATGGGAGAATTCTCAAAGTATTCCTT
AAATTCCGCTTCCGATAAAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQNKIQGVYLVTDRPLCLHHKLEEVVQMAASGGVSLVQLREKDSTSREFLELAIHLKFILSPFQVPLLINDRVDLCLASG
ADGVHLGQTDLPWLEARRILGKDAIIGLSIETKEDFATLTKEDPNPQLEYLAVSPVFDTPTKTNTKEALGLAGVRWLKEK
TDIPVVAIGGINISNAKDVIGAGADMIAVVSAICSAKNPKEATVALRNQF

Sequences:

>Translated_210_residues
MQNKIQGVYLVTDRPLCLHHKLEEVVQMAASGGVSLVQLREKDSTSREFLELAIHLKFILSPFQVPLLINDRVDLCLASG
ADGVHLGQTDLPWLEARRILGKDAIIGLSIETKEDFATLTKEDPNPQLEYLAVSPVFDTPTKTNTKEALGLAGVRWLKEK
TDIPVVAIGGINISNAKDVIGAGADMIAVVSAICSAKNPKEATVALRNQF
>Mature_210_residues
MQNKIQGVYLVTDRPLCLHHKLEEVVQMAASGGVSLVQLREKDSTSREFLELAIHLKFILSPFQVPLLINDRVDLCLASG
ADGVHLGQTDLPWLEARRILGKDAIIGLSIETKEDFATLTKEDPNPQLEYLAVSPVFDTPTKTNTKEALGLAGVRWLKEK
TDIPVVAIGGINISNAKDVIGAGADMIAVVSAICSAKNPKEATVALRNQF

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=176, Percent_Identity=34.6590909090909, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=216, Percent_Identity=35.6481481481481, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LEPBA (B0SE83)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963692.1
- ProteinModelPortal:   B0SE83
- GeneID:   6387379
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2631
- HOGENOM:   HBG754477
- OMA:   CERYKTR
- ProtClustDB:   CLSK2336950
- BioCyc:   LBIF355278:LBF_2631-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22795; Mature: 22795

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIQGVYLVTDRPLCLHHKLEEVVQMAASGGVSLVQLREKDSTSREFLELAIHLKFIL
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
SPFQVPLLINDRVDLCLASGADGVHLGQTDLPWLEARRILGKDAIIGLSIETKEDFATLT
CCCCEEEEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHC
KEDPNPQLEYLAVSPVFDTPTKTNTKEALGLAGVRWLKEKTDIPVVAIGGINISNAKDVI
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
GAGADMIAVVSAICSAKNPKEATVALRNQF
CCCHHHHHHHHHHHCCCCCCHHEEEEECCC
>Mature Secondary Structure
MQNKIQGVYLVTDRPLCLHHKLEEVVQMAASGGVSLVQLREKDSTSREFLELAIHLKFIL
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
SPFQVPLLINDRVDLCLASGADGVHLGQTDLPWLEARRILGKDAIIGLSIETKEDFATLT
CCCCEEEEECCCEEEEEECCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHC
KEDPNPQLEYLAVSPVFDTPTKTNTKEALGLAGVRWLKEKTDIPVVAIGGINISNAKDVI
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
GAGADMIAVVSAICSAKNPKEATVALRNQF
CCCHHHHHHHHHHHCCCCCCHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA