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The map label for this gene is thiM

Identifier: 183222072

GI number: 183222072

Start: 2809519

End: 2810322

Strand: Direct

Name: thiM

Synonym: LEPBI_I2714

Alternate gene names: 183222072

Gene position: 2809519-2810322 (Clockwise)

Preceding gene: 183222071

Following gene: 183222073

Centisome position: 78.05

GC content: 45.9

Gene sequence:

>804_bases
ATGTCTCAACACATTTTAAAAAATACCATCGAAGATTTAGAACTTGTTCGTTCCAAATCACCACTAGTTCATAATATTAC
GAATTACGTAGTGATGAATAACACTGCCAACGCCCTTCTTGCCATTGGTGCCTCACCCATTATGGCACATGCCATCGAAG
AAGTGGAAGAAATGGTGACAATCTGTTCGGCAACTGTCATCAATATTGGAACCCTTTCGGAACCATGGATCCAAAGTATG
GAAAAAGCCGCAAAAAAGGCAGTTTCCTTAGGTAAACCATTGGTTCTTGACCCAGTGGGAGCTGGTGCAAGTAACATTCG
TAATGCGGCAATCCGTAGGATCTTAGATGCGGGGAACCCGACCATTGTCCGTGGAAATGCTTCTGAAATCCTATCCACAC
TCTCTTCCTCTGGGAAAACCAAGGGAGTGGATGCAACGGACTCTTCTGAATCAGCAGTGGAGACTGGCAAATCCTTATCC
AAGGTCACTGGTGGAGTGGTTGTGATCAGTGGAGCCACTGATTTTATCTTAAAAGGTACGGACATGGCCCAAATCTCCAA
TGGAGATGCCTTAATGACAAAGGTCACAGGACTCGGTTGCACTGCCTCTGCACTTTGTGGTGCCTTTGCCGCTGTGCAAA
AAGACCAGTTCCGTGCGGCCACCTCTGCCATGGCGGTCATGGGAATTGCAGGCGAAATGGCAAAATCCAAAACGGCAAGT
CCTGGCAGTTTCCAAGTGGCATTCCTTGATGCCCTGTATGAACTGAATGCAGATACCATTAAACAAAAGTTAAATGCAAA
ATAA

Upstream 100 bases:

>100_bases
CCATAGTTTTTTAAACAAAACTTGGTCGGAATCGGATTTAATCAAAAAAGTGGAAGACGCGATAACGAAGATTGCCTAGG
TTTTCCTAGGAAACCTCCGT

Downstream 100 bases:

>100_bases
AATCCAAGGGGTGTATCTTGTCACAGATAGACCCCTTTGCCTCCACCATAAACTGGAAGAAGTGGTGCAAATGGCCGCTT
CTGGTGGAGTTTCCCTTGTC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSQHILKNTIEDLELVRSKSPLVHNITNYVVMNNTANALLAIGASPIMAHAIEEVEEMVTICSATVINIGTLSEPWIQSM
EKAAKKAVSLGKPLVLDPVGAGASNIRNAAIRRILDAGNPTIVRGNASEILSTLSSSGKTKGVDATDSSESAVETGKSLS
KVTGGVVVISGATDFILKGTDMAQISNGDALMTKVTGLGCTASALCGAFAAVQKDQFRAATSAMAVMGIAGEMAKSKTAS
PGSFQVAFLDALYELNADTIKQKLNAK

Sequences:

>Translated_267_residues
MSQHILKNTIEDLELVRSKSPLVHNITNYVVMNNTANALLAIGASPIMAHAIEEVEEMVTICSATVINIGTLSEPWIQSM
EKAAKKAVSLGKPLVLDPVGAGASNIRNAAIRRILDAGNPTIVRGNASEILSTLSSSGKTKGVDATDSSESAVETGKSLS
KVTGGVVVISGATDFILKGTDMAQISNGDALMTKVTGLGCTASALCGAFAAVQKDQFRAATSAMAVMGIAGEMAKSKTAS
PGSFQVAFLDALYELNADTIKQKLNAK
>Mature_266_residues
SQHILKNTIEDLELVRSKSPLVHNITNYVVMNNTANALLAIGASPIMAHAIEEVEEMVTICSATVINIGTLSEPWIQSME
KAAKKAVSLGKPLVLDPVGAGASNIRNAAIRRILDAGNPTIVRGNASEILSTLSSSGKTKGVDATDSSESAVETGKSLSK
VTGGVVVISGATDFILKGTDMAQISNGDALMTKVTGLGCTASALCGAFAAVQKDQFRAATSAMAVMGIAGEMAKSKTASP
GSFQVAFLDALYELNADTIKQKLNAK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=246, Percent_Identity=41.4634146341463, Blast_Score=181, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325042, Length=281, Percent_Identity=29.1814946619217, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LEPBA (B0SE82)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963691.1
- ProteinModelPortal:   B0SE82
- SMR:   B0SE82
- GeneID:   6388932
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2630
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   LBIF355278:LBF_2630-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27611; Mature: 27480

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: BINDING 48-48 BINDING 124-124 BINDING 170-170 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHILKNTIEDLELVRSKSPLVHNITNYVVMNNTANALLAIGASPIMAHAIEEVEEMVT
CCCHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHH
ICSATVINIGTLSEPWIQSMEKAAKKAVSLGKPLVLDPVGAGASNIRNAAIRRILDAGNP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
TIVRGNASEILSTLSSSGKTKGVDATDSSESAVETGKSLSKVTGGVVVISGATDFILKGT
EEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECC
DMAQISNGDALMTKVTGLGCTASALCGAFAAVQKDQFRAATSAMAVMGIAGEMAKSKTAS
CHHEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGSFQVAFLDALYELNADTIKQKLNAK
CCCEEHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SQHILKNTIEDLELVRSKSPLVHNITNYVVMNNTANALLAIGASPIMAHAIEEVEEMVT
CCHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHH
ICSATVINIGTLSEPWIQSMEKAAKKAVSLGKPLVLDPVGAGASNIRNAAIRRILDAGNP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
TIVRGNASEILSTLSSSGKTKGVDATDSSESAVETGKSLSKVTGGVVVISGATDFILKGT
EEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEECC
DMAQISNGDALMTKVTGLGCTASALCGAFAAVQKDQFRAATSAMAVMGIAGEMAKSKTAS
CHHEECCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGSFQVAFLDALYELNADTIKQKLNAK
CCCEEHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA