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The map label for this gene is gltA2 [H]

Identifier: 183222049

GI number: 183222049

Start: 2788975

End: 2790255

Strand: Reverse

Name: gltA2 [H]

Synonym: LEPBI_I2691

Alternate gene names: 183222049

Gene position: 2790255-2788975 (Counterclockwise)

Preceding gene: 183222050

Following gene: 183222048

Centisome position: 77.51

GC content: 39.5

Gene sequence:

>1281_bases
ATGAGTGAAGTGGAAATCCACATCAAAGGTAAAACATACAAACTTCCAGTAATTACAGGTACTGATGGAAAAGAAGGTAT
CGACCTTACTGAATTTTACCGCAAAACAGGGCTTGTTACTGTTGACCCTGGACTTTTTAATACTGCCCTTGGTTTATCAA
AAGTATCAAGACGTGATCCTGAAAAGGGAGAACTCACATACCGTGGTTATGACCTAAAAGAATTAGCATACCAATCTACT
TTTGTTGAAACATCATTTTTATTAATTTATGGTAACCTCCCCACAAAACAAGAGTTAAGTGATTTTTCTAGTCGCCTCTC
AAAACACTCCATGATCCACGAAGATATGTTAAATCTTTTTGATGGTTTTCCAGGAGTGGCTAACCCACTTGCTGTTTTAT
CTGTAATGGTAACATCATTATCTAGTTATTATTTAGAAGAATACGAAGAGAAGTTGGATATGGGAGTGGATTTAATTGCC
AGATTACTTGCAAAAATTCGTACGATTGCAGCATTCACTTACAAACATGCTGTAGGCCATCCTTTTGTTTACCCTCTCGA
TAAAAATCCATACTGCACAAATTTTTTATACATGATGCACAAACTTCCTGCAGACAACTACAATGTTCCGGAAGAATTTG
ATCGTATTTTAAACCAAATGTGGATCTTACATGCCGACCACGAACAAAATGTATCCAATACCGCCGTACAAGTAGTAGGT
TCGACTCAAGCGAATTTGTTTGCTTCGATCTCGGCTGGAATTATGGCGCAGTGGGGTGCAAGAGAAGGGGGAAGGCCAAC
AGCTGCGATTGGACTCATCGAAGACATCATCAAAACAAAAACTCCTGTGAAAGATTACTTTGAAAGATTCAAACGGGGAG
GGCTTACGATCCGCACCAATGGATTTGGACAAAAAGCATACGATGTCGTCAGTCCAAGAGCACAAGTCGCTCGTGAAATC
ATCAAAGATTTTTATAAGGATCGAAAATTGAGTCCTGTCGAAGACATCGCCCTTCAAATCGATGAAGTGGTTTGGAACGA
TTCATACTTTATGGAGAATCTTTTGTACCCTAACTTAGAATATTATTCTGGTTTGGTGTTCCACACCTTGGGAATTCCTA
AAAACATGTTCTCAGTCATGCAAGTGATTGGAAGGCTTCCTGGATGGCTTGCCCATTGGAGAGAACAAAGAATGAAAGGG
GATTTCTCAAAAGTTCGTCCAAAACAAATTTATGTGGGCGAAAACCAAAGAAAGTACATTCCTATCGCCAACCGTCTCTA
G

Upstream 100 bases:

>100_bases
TTCTCATCCACGATTCCCTTCATTCTGCTCTCGCCCAAGTGTAAATTCTTCTAGATTCCTAACTTACGTTTGATAAGATA
GGAGGGAGAGAGGTCTTTTC

Downstream 100 bases:

>100_bases
AGGATACGAAGGAATCGTGAAACATTATCTCCGTTGGATCACATTCCTTTGGAGTTTGGTACTTTTGACATGTTCAGTGG
CAGAAGTACCGCCAGTTGCG

Product: citrate synthase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEVEIHIKGKTYKLPVITGTDGKEGIDLTEFYRKTGLVTVDPGLFNTALGLSKVSRRDPEKGELTYRGYDLKELAYQST
FVETSFLLIYGNLPTKQELSDFSSRLSKHSMIHEDMLNLFDGFPGVANPLAVLSVMVTSLSSYYLEEYEEKLDMGVDLIA
RLLAKIRTIAAFTYKHAVGHPFVYPLDKNPYCTNFLYMMHKLPADNYNVPEEFDRILNQMWILHADHEQNVSNTAVQVVG
STQANLFASISAGIMAQWGAREGGRPTAAIGLIEDIIKTKTPVKDYFERFKRGGLTIRTNGFGQKAYDVVSPRAQVAREI
IKDFYKDRKLSPVEDIALQIDEVVWNDSYFMENLLYPNLEYYSGLVFHTLGIPKNMFSVMQVIGRLPGWLAHWREQRMKG
DFSKVRPKQIYVGENQRKYIPIANRL

Sequences:

>Translated_426_residues
MSEVEIHIKGKTYKLPVITGTDGKEGIDLTEFYRKTGLVTVDPGLFNTALGLSKVSRRDPEKGELTYRGYDLKELAYQST
FVETSFLLIYGNLPTKQELSDFSSRLSKHSMIHEDMLNLFDGFPGVANPLAVLSVMVTSLSSYYLEEYEEKLDMGVDLIA
RLLAKIRTIAAFTYKHAVGHPFVYPLDKNPYCTNFLYMMHKLPADNYNVPEEFDRILNQMWILHADHEQNVSNTAVQVVG
STQANLFASISAGIMAQWGAREGGRPTAAIGLIEDIIKTKTPVKDYFERFKRGGLTIRTNGFGQKAYDVVSPRAQVAREI
IKDFYKDRKLSPVEDIALQIDEVVWNDSYFMENLLYPNLEYYSGLVFHTLGIPKNMFSVMQVIGRLPGWLAHWREQRMKG
DFSKVRPKQIYVGENQRKYIPIANRL
>Mature_425_residues
SEVEIHIKGKTYKLPVITGTDGKEGIDLTEFYRKTGLVTVDPGLFNTALGLSKVSRRDPEKGELTYRGYDLKELAYQSTF
VETSFLLIYGNLPTKQELSDFSSRLSKHSMIHEDMLNLFDGFPGVANPLAVLSVMVTSLSSYYLEEYEEKLDMGVDLIAR
LLAKIRTIAAFTYKHAVGHPFVYPLDKNPYCTNFLYMMHKLPADNYNVPEEFDRILNQMWILHADHEQNVSNTAVQVVGS
TQANLFASISAGIMAQWGAREGGRPTAAIGLIEDIIKTKTPVKDYFERFKRGGLTIRTNGFGQKAYDVVSPRAQVAREII
KDFYKDRKLSPVEDIALQIDEVVWNDSYFMENLLYPNLEYYSGLVFHTLGIPKNMFSVMQVIGRLPGWLAHWREQRMKGD
FSKVRPKQIYVGENQRKYIPIANRL

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=424, Percent_Identity=36.0849056603774, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=26.0526315789474, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319850, Length=385, Percent_Identity=23.6363636363636, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48574; Mature: 48443

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVEIHIKGKTYKLPVITGTDGKEGIDLTEFYRKTGLVTVDPGLFNTALGLSKVSRRDP
CCCEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCC
EKGELTYRGYDLKELAYQSTFVETSFLLIYGNLPTKQELSDFSSRLSKHSMIHEDMLNLF
CCCCEEECCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGFPGVANPLAVLSVMVTSLSSYYLEEYEEKLDMGVDLIARLLAKIRTIAAFTYKHAVGH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PFVYPLDKNPYCTNFLYMMHKLPADNYNVPEEFDRILNQMWILHADHEQNVSNTAVQVVG
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCHHEEEEC
STQANLFASISAGIMAQWGAREGGRPTAAIGLIEDIIKTKTPVKDYFERFKRGGLTIRTN
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEC
GFGQKAYDVVSPRAQVAREIIKDFYKDRKLSPVEDIALQIDEVVWNDSYFMENLLYPNLE
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHH
YYSGLVFHTLGIPKNMFSVMQVIGRLPGWLAHWREQRMKGDFSKVRPKQIYVGENQRKYI
HHCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEC
PIANRL
CCCCCC
>Mature Secondary Structure 
SEVEIHIKGKTYKLPVITGTDGKEGIDLTEFYRKTGLVTVDPGLFNTALGLSKVSRRDP
CCEEEEECCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCC
EKGELTYRGYDLKELAYQSTFVETSFLLIYGNLPTKQELSDFSSRLSKHSMIHEDMLNLF
CCCCEEECCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DGFPGVANPLAVLSVMVTSLSSYYLEEYEEKLDMGVDLIARLLAKIRTIAAFTYKHAVGH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PFVYPLDKNPYCTNFLYMMHKLPADNYNVPEEFDRILNQMWILHADHEQNVSNTAVQVVG
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCHHEEEEC
STQANLFASISAGIMAQWGAREGGRPTAAIGLIEDIIKTKTPVKDYFERFKRGGLTIRTN
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEC
GFGQKAYDVVSPRAQVAREIIKDFYKDRKLSPVEDIALQIDEVVWNDSYFMENLLYPNLE
CCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHH
YYSGLVFHTLGIPKNMFSVMQVIGRLPGWLAHWREQRMKGDFSKVRPKQIYVGENQRKYI
HHCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEC
PIANRL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]