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The map label for this gene is rob [H]

Identifier: 183221984

GI number: 183221984

Start: 2717136

End: 2718005

Strand: Direct

Name: rob [H]

Synonym: LEPBI_I2624

Alternate gene names: 183221984

Gene position: 2717136-2718005 (Clockwise)

Preceding gene: 183221983

Following gene: 183221985

Centisome position: 75.48

GC content: 35.98

Gene sequence:

>870_bases
ATGGTCTGTGTGAAGCCCAAACCAAATTTGTACCCAGTCTGGAAACAAATTGAAGAACAACTGGAAAAAGAAATTGGTTT
GAATCAAATTGCATTTCTAACAGGGTATAGCGATTGGCACTTCCATCGATTTTTTAAATCCGTACAAAAGGAAAACATAA
AGACCTATATCAAAAGACTACGATTGGAGAAAGCCGCCTATGAATTGAAGATTTCCAATTTCCCAATTTTGGAAATTGGG
ATGGAAGCTGGATTTTCTTCTCACGAGGCATTTACGAAAGCATTCAAACGTGTGATTGGGATCACTCCTTCTGAATTTAG
AAAAAAACACCAGATAAAAATGAAGGATCTCAGTTTATCACAACTCTCACTTCCAAGTGGATTGACAAAACATAGTTTCC
AAAAGAAAACGATATCCCCTTTCTCCATTCTATTCATTCGCCATCTGGGGAGTTATCAATTGTTACCTGGTCCTATTCCC
AATAGCAAAGAAATGATACAACTCAAAACATTGTTTTCAAAATGGGATCTTCTCGAATTCCATCATAAATGGATTGGGAT
TTCGCAAGATGATCCAGATGTCACATGCAAAGATAACATTCGTTTTGATCTTGGTTTTACAATCAGTGAAAAATTTCCAA
GCCCTCCAGATGGTTTTGGAAAACAAACGATCCCTGGTGGGAATTACCTACAAATTCGTTACACTGGTGATTATGAAAAT
TTGCCCATGATTTATGATTGGATTCTGAATCAATATTGTATCGTATCAAAATTAAAATTGAAAAACAATCCACCTTGGGA
GTGTTATCTGAATCCTTTTGAGCCAATTCCCGATAAAAGGATCACAGATATTTACATTCCTTTAGAATGA

Upstream 100 bases:

>100_bases
CACTAGTTGATCCAAACGGAATCGGAATTGATATTGTCCAAGAAAGAAATATGAATTCATAATAAAAATACCATTTGCTT
TCCTTTTTTTAGGACAGTAG

Downstream 100 bases:

>100_bases
TTGAACCCATATGATATCGATTTAGATTCAAACAATAGAGTTTCACTCTGATTGAATCAATTCAATGTTTGGTCTTTTTT
GTTTTCTCCAATAAAATACA

Product: putative AraC-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MVCVKPKPNLYPVWKQIEEQLEKEIGLNQIAFLTGYSDWHFHRFFKSVQKENIKTYIKRLRLEKAAYELKISNFPILEIG
MEAGFSSHEAFTKAFKRVIGITPSEFRKKHQIKMKDLSLSQLSLPSGLTKHSFQKKTISPFSILFIRHLGSYQLLPGPIP
NSKEMIQLKTLFSKWDLLEFHHKWIGISQDDPDVTCKDNIRFDLGFTISEKFPSPPDGFGKQTIPGGNYLQIRYTGDYEN
LPMIYDWILNQYCIVSKLKLKNNPPWECYLNPFEPIPDKRITDIYIPLE

Sequences:

>Translated_289_residues
MVCVKPKPNLYPVWKQIEEQLEKEIGLNQIAFLTGYSDWHFHRFFKSVQKENIKTYIKRLRLEKAAYELKISNFPILEIG
MEAGFSSHEAFTKAFKRVIGITPSEFRKKHQIKMKDLSLSQLSLPSGLTKHSFQKKTISPFSILFIRHLGSYQLLPGPIP
NSKEMIQLKTLFSKWDLLEFHHKWIGISQDDPDVTCKDNIRFDLGFTISEKFPSPPDGFGKQTIPGGNYLQIRYTGDYEN
LPMIYDWILNQYCIVSKLKLKNNPPWECYLNPFEPIPDKRITDIYIPLE
>Mature_289_residues
MVCVKPKPNLYPVWKQIEEQLEKEIGLNQIAFLTGYSDWHFHRFFKSVQKENIKTYIKRLRLEKAAYELKISNFPILEIG
MEAGFSSHEAFTKAFKRVIGITPSEFRKKHQIKMKDLSLSQLSLPSGLTKHSFQKKTISPFSILFIRHLGSYQLLPGPIP
NSKEMIQLKTLFSKWDLLEFHHKWIGISQDDPDVTCKDNIRFDLGFTISEKFPSPPDGFGKQTIPGGNYLQIRYTGDYEN
LPMIYDWILNQYCIVSKLKLKNNPPWECYLNPFEPIPDKRITDIYIPLE

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=96, Percent_Identity=37.5, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790497, Length=93, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081928, Length=98, Percent_Identity=31.6326530612245, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33793; Mature: 33793

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCVKPKPNLYPVWKQIEEQLEKEIGLNQIAFLTGYSDWHFHRFFKSVQKENIKTYIKRL
CEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RLEKAAYELKISNFPILEIGMEAGFSSHEAFTKAFKRVIGITPSEFRKKHQIKMKDLSLS
HHHHHHHEEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCHH
QLSLPSGLTKHSFQKKTISPFSILFIRHLGSYQLLPGPIPNSKEMIQLKTLFSKWDLLEF
HHCCCCCCHHHHCHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HHKWIGISQDDPDVTCKDNIRFDLGFTISEKFPSPPDGFGKQTIPGGNYLQIRYTGDYEN
HHHHCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
LPMIYDWILNQYCIVSKLKLKNNPPWECYLNPFEPIPDKRITDIYIPLE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MVCVKPKPNLYPVWKQIEEQLEKEIGLNQIAFLTGYSDWHFHRFFKSVQKENIKTYIKRL
CEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RLEKAAYELKISNFPILEIGMEAGFSSHEAFTKAFKRVIGITPSEFRKKHQIKMKDLSLS
HHHHHHHEEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCEECCCCHH
QLSLPSGLTKHSFQKKTISPFSILFIRHLGSYQLLPGPIPNSKEMIQLKTLFSKWDLLEF
HHCCCCCCHHHHCHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HHKWIGISQDDPDVTCKDNIRFDLGFTISEKFPSPPDGFGKQTIPGGNYLQIRYTGDYEN
HHHHCCCCCCCCCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
LPMIYDWILNQYCIVSKLKLKNNPPWECYLNPFEPIPDKRITDIYIPLE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]