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The map label for this gene is phrB [C]

Identifier: 183221861

GI number: 183221861

Start: 2578860

End: 2580359

Strand: Direct

Name: phrB [C]

Synonym: LEPBI_I2494

Alternate gene names: 183221861

Gene position: 2578860-2580359 (Clockwise)

Preceding gene: 183221857

Following gene: 183221862

Centisome position: 71.64

GC content: 36.07

Gene sequence:

>1500_bases
ATGAAAAAAGCCCTTCTGATCCTCGGGAACCAACTTTTTGATTTGAGTTCCATCATTCCGAAAGAAAAACGATTGGAATA
CAGTGTCTTTATCAGGGAAGACAATGAGTTGTGTACTTACTATCGATTCCACAAACAAAAGATTTTGTTTTTCTTTTTGG
CAATGCGGGCTTATGCGGAAGAATTAAAGTCTCTCGGATTTTTTGTCCATTACGAACATTATGATCCAAACTCAGAATCG
TATGAAAATCAATTTTTAAATTTCATCCAAGTAAACAATATCCAAGAAATCCATTTTTTTGAAATCGAAGATACATTTTT
TGAAACAAGAATGTTATCCTGTTTTCAAAAAACTGGAATCAAATGGATCCAACACAAATCTCCAATGTTTCTCACTTCGC
GAAATGATTTTAAAGCATATTTACAAACACATAAAAAACCGTTTATGAAAACGTTTTATGAAGCGCAAAGAAAAACTTTC
AACATTCTATTAAATGAAAAGAGTGAACCCATTGGCGGAAAGTGGAGTTTTGATTCTGAAAATCGAAAAAAATTACCCAA
AAATTATTGTGCACCTAACTTACCGAAAATCAAATTCTCAAAAAATGAAATAAAAACCTTTGATACAGTGGATACTCATT
TCCCAAACCATCCAGGGAATACAAAAGATTTTTGGTTACCAACGGATAGAAAAGGAGCAAAACTTTGGCTACAGGATTTT
TTAAAAGAAAGGTTTGTTCAATTTGGTCCTTATGAAGACGCATTCTCACTTGATTTTCCTTTTTTACAACATTCAGTATT
AACTCCATTTTTAAATGTTGGATTATTGACTCCCAAGGAAGTGATCGAATCCACTTTAGACTATGCAAAAAAAAATTCTG
TCCCCATGGAATCGCTGGAAGGTTTTATCCGCCAAATCATTGGTTGGCGTGAATTCATTCGAGGGATTTATCAAAATTTT
GGAGCTAAACAAGAGTCAATAAACTACTTTGGCCACAAACGAAAACTCACCAAACATTGGTATGATGGAACAACCGGGAT
TCCACCTCTCGATTTTGTCATACAAAAATGTAATTTGTATGGTTATGCACATCATATTGAACGATTGATGGTCGTTGGAT
CTCTTATGGTTTTGTTTGAAATTGATCCAAAGGATGCTTACCGCTGGTTTATGGAAATGTTCATTGACTCTTCCGATTGG
GTGATGGGTCCGAATGTTTATGGGATGGCACTCTTCAGTGATGGCGGCATATTTGCCACCAAACCATATATATGTGGATC
CCACTATTATCAAAAAATGGGTTCGTTTCCGAAAGGTGATTGGGAATTGGCTGTGGATGGACTTTACTGGTCCTTCATCG
AAACCCACAAAGATCAGTTTTCCAAAAACCCACGCACTTCTGTCCTCGTTGGAAATTTACACCGAATGCAAAAAGAAAGA
AAGGAGATCATTTTCCACTCGGCAAGGCTCTGGAAGGAAACTCTCACCACACTCAACTAA

Upstream 100 bases:

>100_bases
TCACGGTTTTACTATACCTGATTTTACGGAATTCAACAGAACTTTTCTAAAGAAAGAGAATCATTTTGAATCCGAATGTT
TGTCTAAAAACTAAGCAGAA

Downstream 100 bases:

>100_bases
TCCTGTCTCCTGTTGCTGTGGTTCGTTGCGATTTGGAAAGGCGAATCCGATTCAGAAAAAATTTCCGAACCAGTTGACAA
ATCTAACAAAATGTTTCAGT

Product: putative deoxyribodipyrimidine photo-lyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: Deoxyribodipyrimidine Photolyase-Like Protein; Photolyase; Deoxyribodipyrimidine Photolyase Family Protein; Deoxyribodipyrimidine Photolyase; Photolyase Protein Family Protein; Deoxyribodipyrimidine Photolyase-Like Protein Protein; Photolyase Family Protein; Cryptochrome/Photolyase; DNA Photolyase; Bcct Transporter; Protein Related Deoxyribodipyrimidine Photolyase; Deoxyribodipyrimidine Photo-Lyase; Cryptochrome/Photolyase-Related; Photolyase Related Protein; BCCT Transporter; Alpha-Deoxyribodipyrimidine Photolyase-Related Protein; Deoxyribodipyrimidine Photolyase Related Protein; A Deoxyribodipyrimidine Photolyase-Like Protein; Deoxyribodipyrimidine Photolyase Homolog

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKKALLILGNQLFDLSSIIPKEKRLEYSVFIREDNELCTYYRFHKQKILFFFLAMRAYAEELKSLGFFVHYEHYDPNSES
YENQFLNFIQVNNIQEIHFFEIEDTFFETRMLSCFQKTGIKWIQHKSPMFLTSRNDFKAYLQTHKKPFMKTFYEAQRKTF
NILLNEKSEPIGGKWSFDSENRKKLPKNYCAPNLPKIKFSKNEIKTFDTVDTHFPNHPGNTKDFWLPTDRKGAKLWLQDF
LKERFVQFGPYEDAFSLDFPFLQHSVLTPFLNVGLLTPKEVIESTLDYAKKNSVPMESLEGFIRQIIGWREFIRGIYQNF
GAKQESINYFGHKRKLTKHWYDGTTGIPPLDFVIQKCNLYGYAHHIERLMVVGSLMVLFEIDPKDAYRWFMEMFIDSSDW
VMGPNVYGMALFSDGGIFATKPYICGSHYYQKMGSFPKGDWELAVDGLYWSFIETHKDQFSKNPRTSVLVGNLHRMQKER
KEIIFHSARLWKETLTTLN

Sequences:

>Translated_499_residues
MKKALLILGNQLFDLSSIIPKEKRLEYSVFIREDNELCTYYRFHKQKILFFFLAMRAYAEELKSLGFFVHYEHYDPNSES
YENQFLNFIQVNNIQEIHFFEIEDTFFETRMLSCFQKTGIKWIQHKSPMFLTSRNDFKAYLQTHKKPFMKTFYEAQRKTF
NILLNEKSEPIGGKWSFDSENRKKLPKNYCAPNLPKIKFSKNEIKTFDTVDTHFPNHPGNTKDFWLPTDRKGAKLWLQDF
LKERFVQFGPYEDAFSLDFPFLQHSVLTPFLNVGLLTPKEVIESTLDYAKKNSVPMESLEGFIRQIIGWREFIRGIYQNF
GAKQESINYFGHKRKLTKHWYDGTTGIPPLDFVIQKCNLYGYAHHIERLMVVGSLMVLFEIDPKDAYRWFMEMFIDSSDW
VMGPNVYGMALFSDGGIFATKPYICGSHYYQKMGSFPKGDWELAVDGLYWSFIETHKDQFSKNPRTSVLVGNLHRMQKER
KEIIFHSARLWKETLTTLN
>Mature_499_residues
MKKALLILGNQLFDLSSIIPKEKRLEYSVFIREDNELCTYYRFHKQKILFFFLAMRAYAEELKSLGFFVHYEHYDPNSES
YENQFLNFIQVNNIQEIHFFEIEDTFFETRMLSCFQKTGIKWIQHKSPMFLTSRNDFKAYLQTHKKPFMKTFYEAQRKTF
NILLNEKSEPIGGKWSFDSENRKKLPKNYCAPNLPKIKFSKNEIKTFDTVDTHFPNHPGNTKDFWLPTDRKGAKLWLQDF
LKERFVQFGPYEDAFSLDFPFLQHSVLTPFLNVGLLTPKEVIESTLDYAKKNSVPMESLEGFIRQIIGWREFIRGIYQNF
GAKQESINYFGHKRKLTKHWYDGTTGIPPLDFVIQKCNLYGYAHHIERLMVVGSLMVLFEIDPKDAYRWFMEMFIDSSDW
VMGPNVYGMALFSDGGIFATKPYICGSHYYQKMGSFPKGDWELAVDGLYWSFIETHKDQFSKNPRTSVLVGNLHRMQKER
KEIIFHSARLWKETLTTLN

Specific function: This Enzyme Catalyzes The Light-Dependent Monomerization (300-600 Nm) Of Cyclobutyl Pyrimidine Dimers (In Cis-Syn Configuration), Which Are Formed Between Adjacent Bases On The Same DNA Strand, Upon Exposure To Ultraviolet Radiation. [C]

COG id: COG3046

COG function: function code R; Uncharacterized protein related to deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 59138; Mature: 59138

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALLILGNQLFDLSSIIPKEKRLEYSVFIREDNELCTYYRFHKQKILFFFLAMRAYAE
CCCEEEEECCHHHHHHHHCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELKSLGFFVHYEHYDPNSESYENQFLNFIQVNNIQEIHFFEIEDTFFETRMLSCFQKTGI
HHHHCCEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEEEEHHHHHHHHHHHHHHHHCC
KWIQHKSPMFLTSRNDFKAYLQTHKKPFMKTFYEAQRKTFNILLNEKSEPIGGKWSFDSE
CEECCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
NRKKLPKNYCAPNLPKIKFSKNEIKTFDTVDTHFPNHPGNTKDFWLPTDRKGAKLWLQDF
HHHCCCHHCCCCCCCCEEECCCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCHHHHHHHH
LKERFVQFGPYEDAFSLDFPFLQHSVLTPFLNVGLLTPKEVIESTLDYAKKNSVPMESLE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHH
GFIRQIIGWREFIRGIYQNFGAKQESINYFGHKRKLTKHWYDGTTGIPPLDFVIQKCNLY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GYAHHIERLMVVGSLMVLFEIDPKDAYRWFMEMFIDSSDWVMGPNVYGMALFSDGGIFAT
HHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCCEECCCCEEEEEEECCCEEEE
KPYICGSHYYQKMGSFPKGDWELAVDGLYWSFIETHKDQFSKNPRTSVLVGNLHRMQKER
CCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
KEIIFHSARLWKETLTTLN
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKALLILGNQLFDLSSIIPKEKRLEYSVFIREDNELCTYYRFHKQKILFFFLAMRAYAE
CCCEEEEECCHHHHHHHHCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ELKSLGFFVHYEHYDPNSESYENQFLNFIQVNNIQEIHFFEIEDTFFETRMLSCFQKTGI
HHHHCCEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEEEEEHHHHHHHHHHHHHHHHCC
KWIQHKSPMFLTSRNDFKAYLQTHKKPFMKTFYEAQRKTFNILLNEKSEPIGGKWSFDSE
CEECCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
NRKKLPKNYCAPNLPKIKFSKNEIKTFDTVDTHFPNHPGNTKDFWLPTDRKGAKLWLQDF
HHHCCCHHCCCCCCCCEEECCCCCCCCCCHHCCCCCCCCCCCCEECCCCCCCHHHHHHHH
LKERFVQFGPYEDAFSLDFPFLQHSVLTPFLNVGLLTPKEVIESTLDYAKKNSVPMESLE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHH
GFIRQIIGWREFIRGIYQNFGAKQESINYFGHKRKLTKHWYDGTTGIPPLDFVIQKCNLY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GYAHHIERLMVVGSLMVLFEIDPKDAYRWFMEMFIDSSDWVMGPNVYGMALFSDGGIFAT
HHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCCEECCCCEEEEEEECCCEEEE
KPYICGSHYYQKMGSFPKGDWELAVDGLYWSFIETHKDQFSKNPRTSVLVGNLHRMQKER
CCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
KEIIFHSARLWKETLTTLN
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA