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The map label for this gene is galE [C]

Identifier: 183221419

GI number: 183221419

Start: 2118236

End: 2119243

Strand: Reverse

Name: galE [C]

Synonym: LEPBI_I2037

Alternate gene names: 183221419

Gene position: 2119243-2118236 (Counterclockwise)

Preceding gene: 183221420

Following gene: 183221418

Centisome position: 58.87

GC content: 37.3

Gene sequence:

>1008_bases
ATGAGTGTAAAATCGATATTAGTAACTGGTGGAACGGGATCTTTTGGAAAAAGGTTTATAACAAGATTGATCAAAGCATA
TCCAGATGTAAAACGTGTAGTTGTTTTTTCTCGCGATGAGTTAAAACAGTATGAGATGTCTATGGAGTTCCCTAGTGATG
AATACCCACAAATTCGGTTTTTTATTGGCGATGTCAGAGATAAATCACGACTCCTTTATGCTTTGGAAGGGATTGATACC
GTAATCCATGCAGCAGCGTTAAAACAAGTTCCTGCTGCTGAGTATAATCCATTTGAAGCAATCAAAACAAATGTATTGGG
TGCTCAGAATTTAATTGAATCGTGCATTGAAAAAGGTGTGAAGAATGTTGTCGCACTTTCCACAGATAAAGCAGCAGCTC
CTATCAACTTGTACGGTGCCACTAAGCTTTGTAGTGATAAACTCTTTATTGCTGCAAATCTTTATAAGGGAAATCATGAT
ATTAAATTTTCTGTGGTTCGCTATGGAAATGTTATGGGTTCACGTGGTTCAGTGATTCCTTTTTTTCAAAAGCATAAAGA
AAAAGGATTCTTGCCAATTACTCATCCTGAGATGACTCGTTTTAACATTACGTTAGATGAAGGTGTGGATTTGGTATTCT
ATGCTCTACAAAATATGTGGGGAGGAGAGATCTTTGTACCTAAAATTCCAAGCTATCGAATCTTAGATGTAGCAGAAGCA
GTGGCTCCTGGATTAGAAACAAGGATAGTTGGAATTCGTCCGGGAGAAAAATTACATGAGGAGATGATTACGGAAACGGA
TGCATTGAGTACGCTAGAGTTCGACAAATATTTTGTCATTCTGCCTTCGTTTAAAACTTCATGGGATGTCAAAGATTTTA
AGAAGAAGTTTAACGGGGATTTTTGTAAGGAAAACTTTCGTTATAATAGTGGAGAAAATCACGAATGGTTAAGTGTGGAA
GAAATCCGTAATCTGATCACCTCGAAAGTAAGTGCTGGATTGGTTTGA

Upstream 100 bases:

>100_bases
GAATGGGTTGGAAACACGAAGTGGAGTTGAGAGAAGGAATTCGATTGGCATATGATGATTTCCTGCAAAATGGTGGAATT
GAAAGGTAAGGGAATAAGAG

Downstream 100 bases:

>100_bases
CTATGATTCCTTATGGCAAACAGAATATAAATGAAGCAGATATAAATTCGGTAATTGAAGTTTTAAAATCTGATTTTTTA
ACGCAAGGTCCAGTTGTCGA

Product: putative UDP-glucose 4-epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSVKSILVTGGTGSFGKRFITRLIKAYPDVKRVVVFSRDELKQYEMSMEFPSDEYPQIRFFIGDVRDKSRLLYALEGIDT
VIHAAALKQVPAAEYNPFEAIKTNVLGAQNLIESCIEKGVKNVVALSTDKAAAPINLYGATKLCSDKLFIAANLYKGNHD
IKFSVVRYGNVMGSRGSVIPFFQKHKEKGFLPITHPEMTRFNITLDEGVDLVFYALQNMWGGEIFVPKIPSYRILDVAEA
VAPGLETRIVGIRPGEKLHEEMITETDALSTLEFDKYFVILPSFKTSWDVKDFKKKFNGDFCKENFRYNSGENHEWLSVE
EIRNLITSKVSAGLV

Sequences:

>Translated_335_residues
MSVKSILVTGGTGSFGKRFITRLIKAYPDVKRVVVFSRDELKQYEMSMEFPSDEYPQIRFFIGDVRDKSRLLYALEGIDT
VIHAAALKQVPAAEYNPFEAIKTNVLGAQNLIESCIEKGVKNVVALSTDKAAAPINLYGATKLCSDKLFIAANLYKGNHD
IKFSVVRYGNVMGSRGSVIPFFQKHKEKGFLPITHPEMTRFNITLDEGVDLVFYALQNMWGGEIFVPKIPSYRILDVAEA
VAPGLETRIVGIRPGEKLHEEMITETDALSTLEFDKYFVILPSFKTSWDVKDFKKKFNGDFCKENFRYNSGENHEWLSVE
EIRNLITSKVSAGLV
>Mature_334_residues
SVKSILVTGGTGSFGKRFITRLIKAYPDVKRVVVFSRDELKQYEMSMEFPSDEYPQIRFFIGDVRDKSRLLYALEGIDTV
IHAAALKQVPAAEYNPFEAIKTNVLGAQNLIESCIEKGVKNVVALSTDKAAAPINLYGATKLCSDKLFIAANLYKGNHDI
KFSVVRYGNVMGSRGSVIPFFQKHKEKGFLPITHPEMTRFNITLDEGVDLVFYALQNMWGGEIFVPKIPSYRILDVAEAV
APGLETRIVGIRPGEKLHEEMITETDALSTLEFDKYFVILPSFKTSWDVKDFKKKFNGDFCKENFRYNSGENHEWLSVEE
IRNLITSKVSAGLV

Specific function: Galactose metabolism; third step. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37762; Mature: 37631

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKSILVTGGTGSFGKRFITRLIKAYPDVKRVVVFSRDELKQYEMSMEFPSDEYPQIRF
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCCEEEE
FIGDVRDKSRLLYALEGIDTVIHAAALKQVPAAEYNPFEAIKTNVLGAQNLIESCIEKGV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
KNVVALSTDKAAAPINLYGATKLCSDKLFIAANLYKGNHDIKFSVVRYGNVMGSRGSVIP
HHEEEECCCCCCCCEEECCCHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCH
FFQKHKEKGFLPITHPEMTRFNITLDEGVDLVFYALQNMWGGEIFVPKIPSYRILDVAEA
HHHHHCCCCCEECCCCCCEEEEEEHHCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
VAPGLETRIVGIRPGEKLHEEMITETDALSTLEFDKYFVILPSFKTSWDVKDFKKKFNGD
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEECCEEEEEECCCCCCCCHHHHHHHCCCC
FCKENFRYNSGENHEWLSVEEIRNLITSKVSAGLV
HHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVKSILVTGGTGSFGKRFITRLIKAYPDVKRVVVFSRDELKQYEMSMEFPSDEYPQIRF
CCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCCCCEEEE
FIGDVRDKSRLLYALEGIDTVIHAAALKQVPAAEYNPFEAIKTNVLGAQNLIESCIEKGV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
KNVVALSTDKAAAPINLYGATKLCSDKLFIAANLYKGNHDIKFSVVRYGNVMGSRGSVIP
HHEEEECCCCCCCCEEECCCHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCH
FFQKHKEKGFLPITHPEMTRFNITLDEGVDLVFYALQNMWGGEIFVPKIPSYRILDVAEA
HHHHHCCCCCEECCCCCCEEEEEEHHCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
VAPGLETRIVGIRPGEKLHEEMITETDALSTLEFDKYFVILPSFKTSWDVKDFKKKFNGD
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEECCEEEEEECCCCCCCCHHHHHHHCCCC
FCKENFRYNSGENHEWLSVEEIRNLITSKVSAGLV
HHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]